Column Chromatography i n Three ^ Easy Lessons
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Lesson No.l
Lesson No.2
Begin by pumping your solvent into the column in a smooth concentration or pH gradient. ISCO's new programmed gradient pump, the DIALAGRAD, will handle this perfectly. In fact any simple or complex gradient formed by combining two liquids can be dialed directly into the DIALAGRAD. Program duration and pumping rates are easily adjustable over very broad ranges. No more mixing numerous solutions to guessed-at concentrations.
Now to identify your fractions. Use an ISCO UA-2 or 222 Ultraviolet Analyzer to get quantitative results with no need for further assay. Select linear absorbance, single or dual-beam monitoring at either 254 or 280 mg or both. Absorbance recording minimizes need for base-line compensation and allows direct integration of chart record. As each UV absorbing fraction is recorded it is also automatically deposited in a separate collecting tube.
ISCO INSTRUMENTATION 5624 SEWARD AVE.
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Lesson No. 3
Collecting the effluent is then a simple task when ISCO fraction collectors are used. Choose from any of four basic models offering timed or volumetric control. Some have lift-off reels that can be changed as often as they fill, and when not collecting can be used as convenient storage racks. Extras such as drop counters and volumetric dispensers make things even easier. No after-school sessions necessary when you use ISCO equipment.
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