Integrated Portable Genetic Analysis Microsystem for Pathogen

An integrated portable genetic analysis microsystem including PCR amplification and capillary electrophoretic (CE) analysis coupled with a compact ins...
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Anal. Chem. 2004, 76, 3162-3170

Integrated Portable Genetic Analysis Microsystem for Pathogen/Infectious Disease Detection E. T. Lagally,† J. R. Scherer,‡ R. G. Blazej,† N. M. Toriello,† B. A. Diep,§ M. Ramchandani,§,| G. F. Sensabaugh,§ L. W. Riley,§ and R. A. Mathies*,†,‡

Chemistry Department, University of California, Berkeley, California 94720

An integrated portable genetic analysis microsystem including PCR amplification and capillary electrophoretic (CE) analysis coupled with a compact instrument for electrical control and laser-excited fluorescence detection has been developed. The microdevice contains microfabricated heaters, temperature sensors, and membrane valves to provide controlled sample positioning and immobilization in 200-nL PCR chambers. The instrument incorporates a solid-state laser and confocal fluorescence detection optics, electronics for sensing and powering the PCR reactor, and high-voltage power supplies for conducting CE separations. The fluorescein-labeled PCR products are amplified and electrophoretically analyzed in a gel-filled microchannel in