Isoflavone Content and Composition in Chickpea ... - ACS Publications

Jan 29, 2015 - protein supplement in many countries, including Pakistan,. India ..... sis of variance (ANOVA), followed by post-hoc Dunnett,s t test, ...
0 downloads 0 Views 641KB Size
Subscriber access provided by NEW YORK UNIV

Article

Isoflavones Contents and Composition in Chickpeas (Cicer arietinum L.) Sprouts Germinated under Different Conditions Yue Gao, Yang Yao, Yingying Zhu, and Guixing Ren J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/jf5057524 • Publication Date (Web): 29 Jan 2015 Downloaded from http://pubs.acs.org on February 18, 2015

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

Page 1 of 24

Journal of Agricultural and Food Chemistry

Figure 1. UHPLC−UV profile of 70% aqueous EtOH extracts of germinated chickpeas in GL, and HPLC chromatograms are shown at 280nm. Peaks: 1, glycitein glucoside acetylated; 2, ononin; 3, daidzein; 4, biochanin A glucoside; 5, biochanin A glucoside malonylated; 6, genistein; 7, pseudobaptigenin; 8, formononetin; 9, biochanin A 146x83mm (96 x 96 DPI)

ACS Paragon Plus Environment

Journal of Agricultural and Food Chemistry

Page 2 of 24

1

Isoflavones Contents and Composition in Chickpeas (Cicer arietinum L.) Sprouts

2

Germinated under Different Conditions

3

Yue Gao,† Yang Yao,‡ Yingying Zhu,§ Guixing Ren*,

4 5 6 7





Qilu University of Technology, Daxue Road, Western University Science Park, Jinan,

Shandong, P. R. China, 250353 ‡

Institute of Crop Science, Chinese Academy of Agricultural Sciences, No.80 South

8

Xueyuan Road, Haidian District, Beijing, People’s Republic of China, 100081

9

§

Laboratory of Biophysics and Formulation Engineering, Gembloux Agro-Bio Tech,

10

University of Liège, Passage des Déportés 2, B-5030 Gembloux, Belgium

11

The first two authors contribute equally to this work

12 13 14 15 16 17 18 19 20 21 22

1

ACS Paragon Plus Environment

Page 3 of 24

Journal of Agricultural and Food Chemistry

23

ABSTRACT

24

The influence of different germination conditions on isoflavone contents in chickpea

25

sprouts was investigated in this study. Chickpea sprouts were germinated under different

26

experimental conditions, including germination in the dark (GD), in light (GL), and under

27

ethanol stress (GE) or salt stress (GS) in the dark.

28

isoflavones contents in chickpea sprouts germinated with these various conditions

29

significantly increased (p