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'In order to easily scale up from analytical separations, there is a need for families of packings in different particle sizes, but with the same surface chemistry. Manufacturers should work on tlllS prODlem.
-Klaus Unger to the 1986 Pittsburgh Conference
Baker has done it! Introducing the BAKERBOND™ Universal Matrix. Identical Surface Chemistries on 5μ, 15μ, and 40μ BAKERBOND" ABx Facilitate Rapid Method Development for Scale-up
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Please note scale changes. The void volume peaks of non-retained proteins represent 90-95% of all non-immunoglobulin proteins. Detector sensitivity for all other peaks has been increased twenty-fold to better visualize retained proteins and monoclonal antibodies.
ANALYTICAL CONDITIONS: Column: Mobile Phase: Gradient: Flow Rate: Detection: Peaks: Sample:
Bonded phases with uniform surface chemistry in a full range of particle sizes. BAKERBOND™ Universal Matrix bonded phases offer uniform surface chemistry in particle sizes of 5, 15, and 40 microns.. .to aid in the purification of any biological fluid.. .from characterization, through analysis, to smallscale or large-scale purification.. .quickly, and with high resolution. The family of BAKERBOND silica-based bonded phases includes PEI (anion exchanger), CBX (cation exchanger), HI-Propyl (hydrophobicinteraction), C4 (reversedphase), and MAb™ and ABx™ (antibody purification). They are offered in prepacked HPLC columns (5 and 15 micron) and in bulk (40 micron) and provide superior performance for HPLC, traditional low pressure LC, and batch purification, For technical assistance or ordering information on the entire BAKERBOND Universal Matrix family of bonded phases, call toll free 1-800-JTBAKER. (In New Jersey, 201-859-2151). Or write JT. Baker Chemical Company, 222 Red School Lane, Phillipsburg, NJ 08865.
7.75 χ 100 mm BAKERBOND ABx™, 5 micron, 15 micron, 40 micron Buffer A = 10mM MES, pH 5.6 Buffer Β - 1M NaOAc, pH 7.0 0% Β to 100% B; 60 min. 1.0 ml/min. UV at 280 nm: 2.0, 0.1 AUFS Void Volume: albumins, transferrins, proteases, phenol red Arrow: monoclonal antibody 0.5 ml 10x concentrated cell culture supernatant CIRCLE 18 ON READER SERVICE CARD
J.T.Baker
J.T. Baker Chemical Co. 222 Red School Lane Phillipsburg, NJ 08865 BAKERBOND, BAKERBOND ABx. and BAKERBOND MAb are trademarks of J.T. Baker Chemical Co.
ANALYTICAL CHEMISTRY, VOL. 59, NO. 5, MARCH 1, 1987 · 371 A
