Subscriber access provided by READING UNIV
Article
The linoleic acid: dihomo-#-linolenic acid ratio predicts the efficacy of Zn biofortified wheat in chicken (Gallus gallus) Marija Knez, Elad Tako, Raymond P. Glahn, Nikolai Kolba, Emma de Courcy Ireland, and James C.R. Stangoulis J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b04905 • Publication Date (Web): 23 Jan 2018 Downloaded from http://pubs.acs.org on January 30, 2018
Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.
Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
Page 1 of 29
Journal of Agricultural and Food Chemistry
The linoleic acid: dihomo-γ-linolenic acid ratio predicts the efficacy of Zn biofortified wheat in chicken (Gallus gallus) Marija Knez1*, Elad Tako2, Raymond P. Glahn2, Nikolai Kolba2, Emma de Courcy-Ireland1, James C.R. Stangoulis1 1 2
College of Science and Engineering, Flinders University, GPO Box 2100, Adelaide SA 5001
USDA/ARS, Robert W. Holley Centre for Agriculture and Health, Cornell University, Ithaca, NY 14853 *
Correspondence:
[email protected];
[email protected]; Tel.: +381612769849
1
ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
1
Abstract
2
The amount of Zn absorbed from Zn biofortified wheat material has been determined using
3
an in-vivo model of Zn absorption. Erythrocyte linoleic: dihomo -γ-linolenic acid (LA:
4
DGLA) ratio was used as a biomarker of Zn status. Two groups of chickens (n = 15) were
5
fed different diets: a high Zn (46.5 µg Zn g-1) and a low Zn wheat based diet (32.8 µg Zn
6
g-1). Dietary Zn intakes, body weight, serum Zn, and the erythrocyte fatty acid profile were
7
measured and tissues were taken for gene expression analysis. Serum Zn concentrations
8
were greater in the high Zn group (p
