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Luminescent Copper Nanoclusters as a Specific CellImaging Probe and a Selective Metal Ion Sensor Nirmal Kumar Das, Subhadip Ghosh, Amulya Priya, Sunando Datta, and Saptarshi Mukherjee J. Phys. Chem. C, Just Accepted Manuscript • DOI: 10.1021/acs.jpcc.5b08123 • Publication Date (Web): 05 Oct 2015 Downloaded from http://pubs.acs.org on October 6, 2015
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The Journal of Physical Chemistry
Luminescent Copper Nanoclusters as a Specific Cell-Imaging Probe and a Selective Metal Ion Sensor
Nirmal Kumar Das,§ Subhadip Ghosh,§ Amulya Priya,† Sunando Datta† and Saptarshi Mukherjee §,*
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Department of Chemistry and †Department of Biological Sciences, Indian Institute of Science Education and Research Bhopal Bhopal Bypass Road, Bhauri, Bhopal 462 066, Madhya Pradesh, India
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The Journal of Physical Chemistry
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Abstract Copper nanoclusters (CuNCs) exhibit high tendency to undergo oxidation particularly at sub-nanometer size regime. In the light of overcoming this bottleneck, we have been successful in developing a tri-peptide, (Glutathione, GSH) templated CuNCs which show high bio-compatibility and stability, in spite of being ultrafine in size. These blue emitting CuNCs, possess very promising optical features such as significant quantum yield (QY), and excellent photo-stability. Our cell imaging studies reveal that the CuNCs localize primarily in nuclear membranes of the different cancerous (Hela, MDAMB231 and A549) cells and the cell-viability assay conclusively established their non-toxic nature. Apart from their biological significances, these CuNCs also illustrate their ability to serve as a metal ion sensor; selectively detecting Fe3+ ions in solution at nanomolar concentration regime. This unique luminescent property of the NCs will enable them to serve as label free and versatile probes having several biological and analytical applications.
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The Journal of Physical Chemistry
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Introduction: Metal nanoclusters (NCs) have emerged as a supreme class of materials in the modern
development of nanosciences and nanotechnology possessing some superior optical characteristics in terms of their high photo-stability, quantum yield (QY), biocompatibility and water solublity.1-3 The ultra small size regime of these NCs (
