Magnetically Oriented Phospholipid Bilayers for Spin Label EPR

Mar 19, 1999 - Daniel J. Mayo , Johnson J. Inbaraj , Nidhi Subbaraman , Stuart M. Grosser ... Paresh C. Dave, Johnson J. Inbaraj, and Gary A. Lorigan...
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J. Am. Chem. Soc. 1999, 121, 3240-3241

Magnetically Oriented Phospholipid Bilayers for Spin Label EPR Studies Seth M. Garber,† Gary A. Lorigan,‡ and Kathleen P. Howard*,† Department of Chemistry, Swarthmore College Swarthmore, PennsylVania 19081 Department of Chemistry and Biochemistry Miami UniVersity, Oxford, Ohio, 45056 ReceiVed December 21, 1998 ReVised Manuscript ReceiVed February 24, 1999 This paper reports the development of a model membrane system that spontaneously orients in the applied magnetic field of an EPR spectrometer. There has been a great deal of excitement recently over the use of magnetically oriented phospholipid bilayers (bicelles) for both solution and solid-state NMR spectroscopy.1-4 Here we report nitroxide spin label data from a novel bicelle system which orients in the lower magnetic field of a conventional EPR spectrometer (