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BIOTECHNOLOGY PROGRESS Biotechnology Progress (ISSN 8756-7938) is copublished bimonthly by the American Chemical Society, 1155 16th St., N.W., Washington, DC 20036, and the American I n s t i t u t e of Chemical Engineers, 345 East 47th St., New Yçrk, NY 10017. Second-class postage paid at New York, NY, and additional mailing offices. POSTMASTER: Send address changes to Biotechnology Progress, Member & Subscriber Services, P.O. Box 3337, Columbus, OH 43210 (614) 4473776; (800) 333-9511. Canadian GST Reg. No. R127571347. Printed in the USA.

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BIOTECHNOLOGY PROGRESS

November/December 1994

VOLUME 10, NUMBER 6

Copyright 1994 by the American Chemical Society and the American Institute of Chemical Engineers BIPRET 10(6) 561-658

(1994)

ISSN

8756-7938

EDITOR J E R O M E S. SCHULTZ Center for Biotechnology and Bioengineering University of Pittsburgh Pittsburgh, Pennsylvania 15219 Phone: (412) 383-9712 Fax: (412) 383-9710 ASSOCIATE EDITOR, REVIEWS MICHAEL C. F L I C K I N G E R Institute for Advanced Studies in Biological Process Technology University of Minnesota St. Paul, Minnesota 55108 Phone: (612) 624-9706 Fax: (612) 625-1700 ADVISORY BOARD Fredric Bader Auto Immune James Bailey ΕΤΗ, Zurich Kenneth Bischoff University of Delaware Bruce Dale Texas A&M University Elmer Gaden, Jr. University of Virginia John Gerlt University of Maryland Juan Hong University of California—Irvine Rakesh Jain Harvard Medical School John Jost Genentech, Inc. Alexander Klibanov Massachusetts Institute of Technology Michael Ladisch Purdue University Douglas Lauffenburger University of Illinois, Urbana-Champaign Daryl Lund Rutgers University Janice Phillips Lehigh University Sharon Shoemaker University of California—Davis Michael Shuler Cornell University Gregory Stephanopoulos Massachusetts Institute of Technology JoAnn Stubbe Massachusetts Institute of Technology James Swartz Genentech, Inc. Elizabeth Theil North Carolina State University K. Venkat Phyton Catalytic, Inc Henry Wang University of Michigan Howard Weetall National Institute of Science and Technology

REVIEW

561

New Materials for Biotechnology: Chromatographic Stationary P h a s e s Based on Zirconia Jacek Nawrocki, Christopher J. Dunlap, Peter W. Carr,* and John A. Blackwell A P P L I E D CELLULAR PHYSIOLOGY A N D METABOLIC ENGINEERING

574

Cybernetic Modeling and Regulation of Metabolic P a t h w a y s . Growth on Complementary N u t r i e n t s Jeffrey V. Straight and Doraiswami Ramkrishna*

588

Modeling of Bacterial Growth u n d e r Multiply-Limiting Conditions. Experiments u n d e r Carbon- or/and Nitrogen-Limiting Conditions Jeffrey V. Straight and Doraiswami Ramkrishna*

606

Suboptimal Control of Batch F e r m e n t a t i o n Processes Based on Their Classification by t h e Peculiarity Index Alexandr V. Kazakov* and Sergey L. Ganipol'skiy

611

Microbial Oxidation of Hydrogen Sulfide in a Pilot-Scale Bubble Column Kerry L. Sublette,* Robert P. Hesketh, and Saleem Hasan

615

Biosynthesis of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from E t h a n o l a n d Pentanol by Alcaligenes eutrophus Chang-Ho Park* and Vijay K. Damodaran

CELL C U L T U R E A N D T I S S U E E N G I N E E R I N G

621

T e m p e r a t u r e a n d Growth R a t e Effects on t h e hok/sok Killer Locus for E n h a n c e d Plasmid Stability Kuowei Wu, Deokjin Jahng, and Thomas K. Wood*

630

Stimulatory Effect of a Sulfonylurea Analogue a n d Its Polymer Conjugate on Insulin Secretion from R a t Islets Akihiko Kikuchi, You Han Bae,* and Sung Wan Kim

636

O p t i m u m Infection Conditions for Recombinant Protein Production in Insect Cell (Bm5) Suspension C u l t u r e Junli Zhang, Nicolas Kalogerakis, Leo A. Behie,* and Kostas Iatrou