Mechanism of calf thymus DNA primase: Slow initiation, rapid

Mar 1, 1993 - ACS Nano, ACS Omega, ACS Photonics, ACS Reagent Chemicals, ACS Sens. .... Acyclic Guanosine Analogs Inhibit DNA Polymerases .alpha., .de...
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Biochemistry 1993, 32, 3027-3037

3027

Mechanism of Calf Thymus DNA Primase: Slow Initiation, Rapid Polymerization, and Intelligent Terminationt Robert J. Sheaff and Robert D. Kuchta’ Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309-0215 Received August 20, 1992; Revised Manuscript Received January 11, I993

The mechanism by which calf thymus DNA primase synthesizes R N A primers was examined. Primase first binds a single-stranded DNA template (KD