Biochemistry 1987, 26;1215-1219
1215
Membrane Chloride Transport Measured Using a Chloride-Sensitive Fluorescent Probet Nicholas P. Illsley* and A. S . Verkman* Division of Nephrology and Cardiovascular Research Institute, University of California, San Francisco, California 94143 Received December 1 , 1986; Revised Manuscript Received January 9, 1987
Transport of chloride across cell membranes through exchange, cotransport, or conductive pathways is a subject of great biological importance. Current methods of measurement are restricted in their sensitivity, time resolution, and applicability. A new transport measurement technique has been developed on the basis of the fluorescence quenching by chloride of the dye 6-methoxy-N-(3-sulfopropy1)quinolinium (SPQ). S P Q fluorescence quenching by chloride is rapid (
