Metabolic Characterization of Dairy Cows Treated with Gossypol by

Sep 30, 2017 - Abstract | Full Text HTML | PDF w/ Links | Hi-Res PDF · Solanum nigrum Polyphenol Extracts Inhibit Hepatic Inflammation, Oxidative Stre...
1 downloads 9 Views 1MB Size
Subscriber access provided by LONDON METROPOLITAN UNIV

Article

Metabolic Characterization of Dairy Cows Treated with Gossypol by Blood Biochemistry and Body Fluid Untargeted Metabolome Analyses Chaohua Tang, Kai Zhang, Tengfei Zhan, Qingyu Zhao, and Junmin Zhang J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b03544 • Publication Date (Web): 30 Sep 2017 Downloaded from http://pubs.acs.org on October 1, 2017

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

Page 1 of 37

Journal of Agricultural and Food Chemistry

1

Metabolic Characterization of Dairy Cows Treated with Gossypol by Blood

2

Biochemistry and Body Fluid Untargeted Metabolome Analyses

3

Chaohua Tang§,¶,ǂ, Kai Zhang§,¶ ,ǂ, Tengfei Zhan§,¶, Qingyu Zhao§,¶, Junmin Zhang§,¶,*

4

§

5

Academy of Agricultural Sciences, Beijing 100193, China.

6



7

Nutrition in North China, Ministry of Agriculture, Beijing, China.

8

*

9

86-10-62815890.

10

ǂ

State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese

Scientific Observing and Experiment Station of Animal Genetic Resources and

Corresponding author: email address: [email protected], fax: 86-10-62815537, tel:

These two authors contributed equally to this work.

1

ACS Paragon Plus Environment

Journal of Agricultural and Food Chemistry

11

Abstract

12

To characterize the metabolic disorders of dairy cows treated with gossypol, 12 dairy

13

cows were assigned to either a control group or a treatment group that was fed 1000

14

mg of gossypol per kilogram of dry matter feed for 28 days. Milk quality was

15

adversely affected, as both milk protein and lactose levels were significantly

16

decreased in the gossypol-treated group (3.40% vs. 3.16%, P = 0.044; 5.15% vs.

17

4.91%, P = 0.027; respectively). Plasma samples revealed increases in alanine

18

aminotransferase (P = 0.092), choline esterase (P = 0.02), and glutathione transferase

19

(P = 0.0005), and decreases in glucose (P = 0.076) in the gossypol-treated group.

20

Mass spectrometry-based comparative metabolomic analyses showed reduced

21

concentrations of the gluconeogenesis precursor L-glutamine (P = 0.047), with

22

significant decreases (P