Supporting information for: Metabolic Epoxidation of an α,β-Unsaturated Oxime Generates Sensitizers of Extreme Potency. Are Nitroso Intermediates Responsible? †
‡
Moa Andresen Bergström, Kristina Luthman, and Ann-Therese Karlberg † ‡
†*
Department of Chemistry, Dermatochemistry and Skin Allergy, Göteborg University, SE-412 96 Göteborg, Sweden Department of Chemistry, Medicinal Chemistry, Göteborg University, SE-412 96 Göteborg, Sweden
Table of Contents Results from the local lymph node assay (LLNA) Results from the mouse ear swelling test (MEST)
S1 S2-3
Fragment assignments for Pro-His-Cys-Lys-Arg-Met (PHCKRM) and covalently bound 3a or 4 (*)
S4
Reactivity of α,β-Epoxy Oxime 3a Toward PHCKRM Total ion current (TIC) and extracted ion (XIC) chromatograms of conjugates formed from 3a (1 eq.) and PHCKRM (1 eq.) S5 TIC and XIC chromatograms of conjugates formed from 3a (5 eq.) and PHCKRM (1 eq.) S6 Mass spectra of monohaptenated conjugates formed from 3a (1 eq.) and PHCKRM (1 eq.) S7 Mass spectra of dihaptenated conjugates formed from 3a (5 eq.) and PHCKRM (1 eq.) S8 – S9 Mass spectra of trihaptenated conjugates formed from 3a (5 eq.) and PHCKRM (1 eq.) S10 – S11 Mass spectra of tetrahaptenated conjugates formed from 3a (5 eq.) and PHCKRM (1 eq.) S12
Reactivity of α,β-Epoxy Oxime 4 Toward PHCKRM TIC and XIC chromatograms of conjugates formed from 4 (1 eq.) and PHCKRM (1 eq.) TIC and XIC chromatograms of conjugates formed from 4 (5 eq.) and PHCKRM (1 eq.) Mass spectrum of the monohaptenated conjugate formed from 4 (1 eq.) and PHCKRM (1 eq.) Mass spectrum of the dihaptenated conjugate formed from 4 (5 eq.) and PHCKRM (1 eq.) Mass spectrum of the trihaptenated conjugate formed from 4 (5 eq.) and PHCKRM (1 eq.) Mass spectra of tetrahaptenated conjugates formed from 4 (5 eq.) and PHCKRM (1 eq.)
*
S13 S14 S15 S16 S17 S18
To whom correspondance should be addressed. E-mail:
[email protected]; Tel: +46-31-7724726; Fax: +46-31-7723840
Results from the local lymph node assay (LLNA)a: Sensitization experiments of 3a, 4, 5 and 7a. [3H]thymidine incorporation (dpm/lymph node)
SIb
1116 2421 8773 17393 17941 18896
2.17 7.86 15.6 16.1 16.9
Control 0.01 0.1 1 5 10
834 1259 2101 8611 13175 13279
1.51 2.52 10.3 15.8 15.9
Control 0.06 0.6 6
394 653 682 867
Control 0.01 0.1 1 5 15
885 1088 805 698 984 896
compd and test concn (% w/v) 3a Control 0.01 0.1 1 5 10
EC3 valuec % w/v mM 0.020 1.1
classificationd extreme
4
5
0.016
0.88
extreme
>6.0
>330
-
>15
>770
weak or non-sensitizing
1.66 1.73 2.20
7a 1.23 0.91 0.79 1.11 1.01
a
Groups of mice (n = 3) recieved 25 µl of the test chemical dissolved in vehicle (acetone:olive oil 4:1), on the dorsum of both ears for 3 consecutive days. Control animals were treated in the same way with the vehicle alone. All mice were injected intravenously 5 days after the first treatment, with 250 µl of PBS containing 20 µCi of [3H] methyl thymidine. Five hours later draining auricular lymph nodes were excised and pooled for each group, and a single cell suspension of lymph node cells was prepared. The thymidine incorporation was measured by β-scintillation counting. b
The stimulation indices (SI) correspond to the increase in thymidine incorporation of treated groups relative to vehicle-treated controls. c
EC3 values (the estimated concentration required to induce an SI of 3) were calculated using linear interpolation. d
The sensitizing potency of the test compounds were classified according to the following: EC3
