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18
C&ENSept. 18, 1972
oib Woodward Building 15th and H Streets, N.W wash.ngton D.C. 20005
Detecting D-lysergic acid diethylamide (LSD) in the urine of a person who has taken the drug is no small problem. The drug is ordinarily taken in microgram amounts, then becomes extremely dilut ed as it is distributed throughout the body. Now scientists at Collaborative Research, Inc., Waltham, Mass., have devised a method that will detect LSD in urine at a level of as little as 10~ 1 3 mole (0.1 picomole). The new method, a radioimmunoas say technique, was developed by a group at the biomedical research and develop ment company that included Paul R. Kelley, immunologist Stephanie E. Sher, and was headed by chemist Alison Taunton-Rigby. The two-year project was carried out under a contract from the Bureau of Narcotics and Dangerous Drugs. The scientists worked out a way to obtain antibodies to LSD. LSD is not antigenic. But human serum albumin is found in rabbits. By attaching LSD to human serum albumin it was possible to raise antibodies to LSD in rabbits and use the sera containing them as reagents. The assay uses an LSD compound labeled with radioisotope iodine-125. The LSD in an unknown sample dis places the radioactive LSD from its binding sites on the LSD antibody and the displacement is measured. Highly specific to LSD, the assay has been tested with 25 other structurally related compounds and these did not react. The compounds included am phetamine, nicotinic acid, D,L-metanephrine Af,iV-diethyl tryptamine, and D,L-ephedrine. D-Lysergic acid, the nonhallucinogenic acid from which LSD is derived, does not react either. Al though other compounds which might also be found in some urine samples such as ethanol and A ^tetrahydrocan nabinol haven't yet been tested, it is unlikely that they will interfere, the scientists say. In announcing the new technique, Dr. Theresa Harwood, chief of the biological research branch of the Bureau of Nar cotics and Dangerous Drugs, noted that availability of the quantitative as well as qualitative method will open many areas of investigation with LSD. It will be possible, for example, to study distri bution of LSD throughout an animal body, follow its metabolism, measure the rate and patterns of its excretion, correlate blood levels of the drug with pharmacological effects and behavior, and study duration of drug concentra tion and accumulation in the body. The new method, Dr. Harwood says, will also be an asset in diagnosing bi zarre behavior in the emergency room of a hospital so that proper treatment can be applied if LSD is the culprit. It will also be an aid in urinalysis of subjects in methadone maintenance programs.
Cleveland, May 1 3 - 1 5 . 1 9 7 3
FIFTH
CENTRAL REGIONAL MEETING CALL FOR PAPERS Contributed papers are invited in all branches of chemistry Symposium topics are: Newer Analytical Techniques in Clinical Chemistry, Macromolecular Structure, Activities of the ACS Division of Chemical Education, Sulfur Chemistry, Reference Aids, Optical Activity in Metal Com plexes and Metalloproteins, Metabolic Regulation, Biological Membranes, Reactive Inter mediates in Organic Chemistry, Chemistry of Transition Metal Carbon Bonds, Diffusion, Photo chemical Processes, Modern Instrumentation for Chemical Technicians. The meeting will include the annual May conference of the Cleveland Sections of SAS (Analytical Division), ACS, the Morley Award banquet and a chemical exhibition. Abstracts—Standard ACS form Deadline—Dec. 3 1 , 1972 Send to—Dr. John Fackler, Jr. j Dept. of Chemistry | Case Western Reserve Univ. I Cleveland, Ohio 44106
