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A Method for Electrochemical Detection of Brain Derived Neurotrophic Factor (BDNF) in plasma. Marina Bockaj, Barnabas Fung, Michael Tsoulis, Lauren (Warren) Foster, and Leyla Soleymani Anal. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.analchem.8b01642 • Publication Date (Web): 22 Jun 2018 Downloaded from http://pubs.acs.org on June 25, 2018
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Analytical Chemistry
A Method for Electrochemical Detection of Brain Derived Neurotrophic Factor (BDNF) in Plasma Bockaj M.1,2, Fung B.1, Tsoulis M.1, Foster WG.1,2,†, and Soleymani L1,†.
Department of Engineering Physics School of Biomedical Engineering McMaster University
Department of Obstetrics & Gynaecology Michael G. DeGroote School of Medicine McMaster University
†
Address for correspondence: Dr. Leyla Soleymani
[email protected] or
Dr. Warren Foster
[email protected] Key words: Endometriosis, point-of-care diagnosis, electrochemical biosensing, BDNF, plasma.
ACS Paragon Plus Environment
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ABSTRACT
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Currently, a blood test for the diagnosis of endometriosis, a common estrogen-dependent
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gynecological disease, does not exist. Recent studies suggest that circulating
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concentrations of brain derived neurotrophic factor (BDNF) have potential for the
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diagnosis of endometriosis. However, at present BDNF can only be measured by ELISA
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which requires a clinic visit, a routine blood sample, and laboratory testing. Therefore,
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we developed a point-of-care device (EndoChip) for use with small blood volumes that
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can be collected through a finger prick. Specifically, the presented device is a polymer-
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based chip with a nanoporous, wrinkled gold film acting as the electrode/sensing layer,
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allowing for the electrochemical detection of BDNF in plasma. Increasing concentrations
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of BDNF (0.1 – 2.0 ng/ml) induced significant differences in redox current. The
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biosensor produces a signal readout in a matter of seconds, and is ideal for realizing
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multiplexing. Blood samples were collected from women (n=20) with chronic pelvic pain
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undergoing a diagnostic laparoscopy. Plasma BDNF concentrations measured by
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commercial ELISA were positively correlated (r2=0.8033; p
