Rapid Paper
Electrophoresis
New ΓΠISC0 Unit Uses Organic Buffers, Completes Separations in 1 Hour Misco's a d v a n c e d multiple-cell electrophoretic techniques feature organic buffers for b o t h analytical a n d clinical investigations. Usually within a n h o u r , u n k n o w n c o m p o u n d s c a n b e completely characterized o r k n o w n samples separated for quantitative analysis. Just a s rapidly, this flexible unit will purify u p t o a few milligrams of a n y c o m p o u n d that c a n b e separated b y electrophoresis. T h e Misco a p p a r a t u s a n d special o r g a n i c buffers were developed b y D r . H a r o l d T. H^I.'II>HB,TW1 G o r d o n of t h e University of California. Buffer: Dimethylaminoethanol-formic acid in 30% Formamide. P a r t i c u l a r success has been achieved with pH: 4.0 Ionic strength: 0.18 small o r g a n i c molecules s u c h a s a m i n o Paper: Whatman No. 4 Gradient: 15 volts/cm. acids, c a r b o h y d r a t e s a n d peptides.* Current: 1.5 ma./cm. Time: One hour. Cooling: None. Sample: 20 > of each amino acid, in one L volume.
Amaranth
Aspartic
Brilliant Blue
Glutamic
Alanine
T h e G o r d o n - M i s c o t e c h n i q u e offers t h r e e key i n n o v a t i o n s — o r g a n i c buffers in f o r m a m i d e , a m u l t i - c h a m b e r cell, a n d r e f e r e n c e d y e s . T h e buffers m i n i m i z e evaporation, allow m u c h higher voltages. T h e m u l t i - c h a m b e r cell is designed f o r r u n n i n g 1 t o 5 samples simultaneously at 5 different p H values from 3.3 t o 9 . 3 . Reference dyes ( s u c h as A m a r a n t h a n d Apolon) assure reproducible relative mobility values, often m a k e possible t h e calculation of t h e p K a n d molecular weight of t h e u n k n o w n . Misco will b e h a p p y t o send y o u m o r e information about rapid p a p e r electro phoresis a n d a r e p r i n t of t h e W e r u m G o r d o n - T h o r n b u r g article. Please a d dress D e p t . 0 4 - 2 .
Apolon
^•Alanine
*Rapid Paper Inophoresis Using Organic Buffers in Water-Formamide and WaterUrea. L. N. Werum, H. X Gordon, W. Thornburg. / . Chromatography (in press).
-Aminobutyric
MICROCHEMICAL 1825 EASTSHORE
HIGHWAY
Specialists in Micro
SPECIALTIES CO. BERKELEY
Circle No. 61 on Readers' Service Card 80 A
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ANALYTICAL CHEMISTRY
10, C A L I F O R N I A
Techniques for 22 Years
PITTSBURGH EXPOSITION Booths:
79,80,414,415
BECKMAN INSTRUMENTS, I N C . Scientific and Process Instruments D i v . 2 5 0 0 Fullerton Rd. Fullerton, Calif.
Exhibiting: Beckman's new TJV Spectro photometer, Gas Chromatographs, New Beckman p H Meters, Preparative Gas Chromatograph, Prism-Grating IR-7 Infrared Spectrophotometer, I R - 5 Lowcost I R Spectrophotometer, New Beckman Laboratory Hygrometers, New Beckman Laboratory Recorders, F a r UV Recording Spectrophotometer, New Beckman Blood p H Analysis System, Teflon Tube Connectors, Solution Metering Pumps. Representatives at Exhibit: J . M . Manypenny, J . P . Addonizio, V. J . King, J . A. Challacombe, G. A. Green, C. D. Kennedy, E . M . Melaun, C. M . Casey, R . A. Piper, Frank Kelly, R. E . Goettmann, Joseph Ashley, H. Noebels, J . McCollum, M . R. Bumell, W. S. Gallaway, D . Herbert, Lew Hodgson, Theron Johns, W. I. Kaye, Robert Lawson, Joseph Lewis, R. J . Manning, R. E . Moody, J. C. Sternberg, Allan Strickler, D . G. Tipotsch, G. Turner, W. Ulrich, H . Sloane, J. Barnyak, F . Christ, W. Slee. General Product Line: Manufacturer of Lab oratory and Process Instruments, includ ing laboratory and industrial oxygen analyzers, laboratory and industrial gas chromatographs, continuous turbidemeters, air pollution analysis systems, chemical analysis systems, non-dispersive infrared gas analyzers, thermal conductivitv analyzers.
Booth: 64 BENDIX A V I A T I O N CORP. Cincinnati Division 3 1 3 0 Wasson Rd. Cincinnati 8 , O h i o
Exhibiting: Bendix Time-of-Flight mass spectrometer, Model 12-101, equipped with recording accessory, t h e analog output system, and new solid sample inlet system. Representatives at Exhibit: D a n Har rington, Jack Francis, Marion Munroe, Don Damoth. General Product Line: Mass spectrometer, electron microscope, multi-channel pulse height analyzer, voltage controlled os cillator, viscometer, nuclear reactor con trol systems, dosimeters.
Booth: 85 BETHLEHEM APPARATUS C O . Front & Depot Sts. Hellertown, Pa.
Exhibiting: Two new products—Bethlehem glassblowing kit for chemists, physicists, engineers called upon to do glassworking intermittently. Kit contains manual of instructions describing mechanical aids t o supplement kit. T h e second new prod uct: the sharp flame hand torch (gasoxygen) is surface-mixing and has a wide flame range without requiring tip change. This product will be demonstrated along with Bethlehem bench burner. Also exhibiting Bethlehem Lab-Lathe for glass working, other surface mixed gas oxygen burners, Bethlehem Seal Crib for glass tubing. Mercury cleaning equip-
