Subscriber access provided by UNIVERSITY OF TOLEDO LIBRARIES
Synthesis of fluorescent nanoscale salts/MOFs for live-cell imaging Udai Pratap Singh, NEETU SINGH, RITU VARSHNEY, Partha Roy, and Ray J Butcher Cryst. Growth Des., Just Accepted Manuscript • DOI: 10.1021/acs.cgd.7b01574 • Publication Date (Web): 28 Mar 2018 Downloaded from http://pubs.acs.org on March 28, 2018
Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.
is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
Page 1 of 29 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60
Crystal Growth & Design
1
Synthesis of fluorescent nanoscale salts/MOFs for live-cell imaging
2
Udai P. Singha* Neetu Singha, Ritu Varshneyb, Partha Royb and Ray J. Butcherc
3
a
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee – 247667, India. Email:
[email protected]; Tel: +91-1332-285329
4 5
b
6
India.
7
c
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee – 247667,
Department of Chemistry, Howard University, Washington, DC 20059, USA
8 9
ABSTRACT
10
The reaction between disulfonic acid i.e. 1,5-naphthalenedisulfonic acid (NDS),
11
Cu(NO3)2.3H2O and variety of ditopic pyridine analogues viz., 9, 10-bis((E)-2-(pyridin-4-
12
yl)vinyl)anthracene (B4PVA), 9, 10-bis((E)-2-(pyridin-3-yl)vinyl)anthracene (B3PVA) and 3,3'-
13
(peroxybis(methylene))dipyridine (3PBP) resulted in the formation of salts/MOFs i.e. [NDS2-
14
.B4PVA2+.S] (FNP 1a), [NDS2-.3PBP2+.MeOH.H2O] (FNP 1b), [Cu.NDS.B4PVA] (FNP 1c),
15
[Cu.NDS.B3PVA] (FNP 1d) and [Cu.NDS.3PBP] (FNP 1e) by diffusion method where S in
16
FNP 1a is squeezed solvent molecule. Sulfonates with six oxygen atoms involve in coordination
17
with metal center and formed hydrogen bond interactions. X-ray structural analysis showed
18
supramolecular architecture of the salts/MOFs where ligands act as a linear linker. The
19
approximate particle size was calculated by PXRD pattern of the compounds using Debye
20
Scherrer equation. The morphological characterization of the FNPs were performed by using
21
scanning electron microscopy (SEM). The emission properties of these salts/MOFs may due the
22
intraligand charge transfer (ILCT) and ligand to metal charge transfer (LMCT) phenomena.
23
These nanocrystals were evaluated in live-cell imaging assays using HeLa and MCF-7 as a
24
model cell-line.
25
INTRODUCTION
26
Metal organic frameworks (MOFs) are crystalline porous hybrid inorganic-organic materials
27
prepared from an assembly of metal ions with organic linkers through strong covalent bonds1.
28
Their define porosity and tunable chemical functionality make them attractive for the
29
applications like gas storage2-6, catalysis7-10 and separation11-15 etc.. Apart from their use as bulk
30
materials in above applications, MOFs are also potential candidates in bioimaging for living
31
cells. However, because of their micro porosity (generally, pore size of
