Multilayer Adsorption of Cytochrome c on Mica ... - ACS Publications

sured to within lo-' N by using a double-cantilever spring and the surface separation D ... interferometrically by using fringes of equal chromatic wa...
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Langmuir 1990, 6, 1704-1708

Multilayer Adsorption of Cytochrome c on Mica around Isoelectric pH P. K6kicheff,* W. A. Ducker, B. W. Ninham, and M. P. Pilenit Department of Applied Mathematics, Research School of Physical Sciences, Australian National University, G.P.O. Box 4, Canberra A.C.T. 2601, Australia, and Universitg Pierre-et-Marie-Curie (Paris V n , S.R.I., Bdtiment de Chimie-Physique, 11, rue Pierre-et-Marie-Curie, 75231, Paris Cedex 05, France Received June 4, 1990. In Final Form: September 4, 1990 The force as a function of separation has been measured between two molecularly smooth mica surfaces immersed in a dilute solution of cytochrome c near the isoelectric pH. The force-distance profile shows oscillations at small separations (