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Bioactive Constituents, Metabolites, and Functions

New #-Lactone with Tea Pathogenic Fungus Inhibitory Effect from Marine-derived Fungus MCCC3A00957 Xi-Xiang Tang, Xia Yan, Wen-Hao Fu, Lu-Qi Yi, Bo-Wen Tang, Li-Bo Yu, Meijuan Fang, Zhen Wu, and Ying-Kun Qiu J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.9b00228 • Publication Date (Web): 20 Feb 2019 Downloaded from http://pubs.acs.org on February 21, 2019

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Journal of Agricultural and Food Chemistry

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New β-Lactone with Tea Pathogenic Fungus Inhibitory Effect from

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Marine-derived Fungus MCCC3A00957

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Xi-Xiang Tang, 1, † Xia Yan, 3, † Wen-Hao Fu, 2 Lu-Qi Yi, 2 Bo-Wen Tang, 2 Li-Bo Yu, 1 Mei-Juan

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Fang, 2 Zhen Wu, 2 and Ying-Kun Qiu 2, *

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1

Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography State,

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Ministry of Natural Resources, Da-Xue Road, Xiamen 361005, China;

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E-Mails: [email protected] (X.-X. Tang), [email protected] (L.-B. Yu)

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Fujian Provincial Key Laboratory of Innovative Drug Target Research, School of Pharmaceutical Sciences, Xiamen University, South Xiang-An Road, Xiamen, 361102, China;

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E-Mails: [email protected] (W.-H. Fu); [email protected] (L.-Q. Yi); [email protected]

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(B.-W. Tang); [email protected] (M.-J. Fang); [email protected] (Z. Wu);

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[email protected] (Y.-K. Qiu)

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Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Ningbo University, Ningbo 315832, China; [email protected] (X.Y.)

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* Corresponding author:

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Tel.: +86-592-2189868; Fax: +86-592-2189868;

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E-mail address: [email protected]

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These authors contributed equally to this paper

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ABSTRACT

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Fusarium solani H915 (MCCC3A00957), a fungus originating from mangrove sediment, showed

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potent inhibitory activity against tea pathogenic fungus Pestalotiopsis theae. Successive

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chromatographic separation on an ethyl acetate (EtOAc) extract of F. solani H915 resulted in the

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isolation of five new alkenoic diacid derivatives, fusarilactones A–C (1–3), fusaridioic acids B (4) and

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C (5), in addition to seven known compounds (6–12). The chemical structures of these metabolites

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were elucidated on the basis of UV, IR, HR-ESI-MS and NMR spectroscopic data. The antifungal

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activity of the isolated compounds was evaluated. Compounds with a β-lactone ring (1, 2 and 7)

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exhibited potent inhibitory activities, while none of the other compounds show activity. The ED50

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values of the compounds 1, 2 and 7 were 38.14 ± 1.67 µg/mL, 42.26 ± 1.96 µg/mL, and 18.35 ± 1.27

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µg/mL respectively. Additionally, inhibitory activity of these compounds against 3-hydroxy-3-

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methylglutaryl-CoA (HMG-CoA) synthase gene expression was also detected by real time RT-PCR.

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Results indicated that compounds 1, 2 and 7 may inhibit the growth of P. theae by interfering with the

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biosynthesis of ergosterol by down-regulating the expression of HMG-CoA synthase.

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KEYWORDS: Fusarium solani H915; fusarilactones A, B, C; fusaridioic acids B, C; HMG-CoA

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synthase; tea pathogenic fungus inhibitory effect.

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Journal of Agricultural and Food Chemistry

INTRODUCTION

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Tea [Camellia sinensis O. Kuntze] is an important economic crop in many countries. However,

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the growth and production of tea can be severely disturbed by various factors, especially fungal

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diseases that infects the leaves. 1-2 Infected tea leaves often exhibit severe damage of the blade tissue

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and discoloration of the leaf, such as blight (Exobasidium vexans Massee), grey blight (Pestalotiopsis

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theae (Sawada) Steyaert), brown blight (Colletotrichum camelliae Massee), sooty mold (Capnodium

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theae Boedijn), and red rust (Cephaleuros parasiticus Karst). 1, 3-7

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Fungicides and biocontrol agents have demonstrated potential against these pathogenic fungi.

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Synthetic chemical fungicides such as thiophanatemethyl, carbendazim and contact fungicides such as

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mancozeb and, copper oxychloride have been effectively used in the field.

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including Bacillus subtilis, 9 Trichoderma viride, 10 Ochrobactrum anthropic, 11 and Streptomyces spp.

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12

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fungicides can lead to many problems, such as phytopathogen resistance, food safety threat and

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environmental pollution. 13-15 Therefore, the development of new natural product derivative fungicides

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is of great importance to overcome these shortcomings.

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Biocontrol agents,

have also been used in antagonizing these tea pathogenic fungi. However, long-term use of chemical

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To date there have only been a few reports on active compounds against tea pathogenic fungi.

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Essential oil-based β-methoxyacrylate derivatives have been synthesized and showed remarkable

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inhibitory activities against P. theae. 16 Major tea leaf volatile constituents, including geraniol, linalool,

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methyl salicylate, benzyl alcohol, and 2-phenylethanol were found to exhibit significant antifungal

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activities toward Colletorichum camelliae Massea.

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temperature, pressure, salt concentration and pH, are known to be a rich resource for bioactive natural

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products.

18-19

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Marine environments, with a wide range of

Previously, we identified a new macrolactin

20

form Bacillus subtilis B5, a bacteria

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isolated from sea sediment 3000 metres below the Pacific Ocean, which exhibited antifungal activity

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against P. theae and Colletorichum gloeosporioides. Mangrove ecosystems are coastal wetlands with

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high primary production rates, equal to those of tropical humid evergreen forests and coral reefs.

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Sediment microorganisms help create and maintain mangrove ecosystems by decomposition of organic

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matter and are critical for the cycling of nutrients. 22 Mangrove-associated microorganisms are thought

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to be of great value for obtaining bioactive natural products. 23-24 Endophytic Fusarium sp have also

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been shown to produce various secondary metabolites, such as cyclic pentapeptides, cyclic

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lipopeptides, 25 α-pyridone derivatives, ceramide derivatives, 15 azaphilone derivatives, 26 isocoumarin

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derivatives,

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These metabolites showed various bioactivities. We herein report the isolation, structural

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determination, and antifungal activity of the metabolites from an extract of Fusarium solani H915

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(MCCC3A00957), a fungus originating from mangrove sediment.

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trehalose-containing glycolipids,

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and polyketide-derived isoquinoline alkaloids.

21

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MATERIALS AND METHODS

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General experimental procedures

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Open-column separations were carried out using silica gel (Yantai Chemical Industry Research

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Institute, Yantai, China) or Cosmosil 75 C18-OPN (75 μm, Nakalai Tesque Co. Ltd., Kyoto, Japan).

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The preparative HPLC was performed with a preparative Cosmosil ODS column (250 mm × 20.0 mm

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i.d., 5 m, Cosmosil, Nakalai Tesque Co. Ltd., Kyoto, Japan), via a Varian binary gradient LC system

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(Varian Inc. Corporate, Santa Clara, CA, USA). The HR-ESI-MS spectra were acquired on a Q-

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Exactive Mass spectrometer (Thermo Fisher Scientific Corporation, Waltham, MA, USA). UV spectra

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were recorded on a Shimadzu UV-260 spectrometer (Shimadzu Corporation, Tokyo, Japan). IR spectra

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in KBr pellets were determined using a Perkin-Elmer 683 infrared spectrometer (PerkinElmer, Inc.,

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Waltham, MA, USA). Optical rotations were measured on a JASCO P-200 polarimeter (JASCO

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Corporation, Tokyo, Japan), equipped with a 5-cm cell. The NMR spectra of the compounds dissolved

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in DMSO-d6 were run on a Bruker Avance III 600 FT NMR spectrometer (Bruker Corporation,

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Billerica, MA, USA).

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Circular Dichroism (CD) spectra and Electronic Circular Dichroism (ECD) Calculations

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The circular dichroism (CD) spectra were acquired on a Chirascan circular dichroism

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spectrometer (Applied Photophysics Ltd., Leatherhead, UK). The theoretical electronic circular

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dichroism (ECD) spectra of the isolated compounds were calculated on the basis of the relative

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configurations determined by their NOESY spectra and J values in the 1H NMR. Conformational

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analyses and density functional theory (DFT) calculations were used to generate and optimize the

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conformers with energy. The ECD calculations were performed following a method descripted

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previously [25].

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Fungus and carbohydrate fermentation

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The H915 strain was isolated from the mangrove sediments at the estuary of Zhangjiangkou

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Mangrove National Nature Reserve (23° 55′35.37″N, 117°24′50.93″E), Fujian province, China using

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a tablet pour method. The internal transcribed spaces (ITS) region was amplified and sequenced by

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using the general primers ITS1 and ITS4. The ITS region of the fungus was a 576 bp DNA sequence

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(GenBank accession number KY978583) which had 99% identity to Fusarium solani. The strain was

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deposited at the China Center for Type Culture Collection (CCTCC, M2017150) and Marine Culture

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Collection of China (MCCC, 3A00957). Carbohydrate fermentation was carried out by subculturing

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the fungus onto a rice-artificial sea water medium, incubated at 28°C for 30 days in a standing position.

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P. theae (ITS GenBank accession number HQ832793) was isolated from foliar lesions of the tea leaf

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and its pathogenicity to tea leaves was verified both in vitro and in vivo (unpublished data).

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Extraction and isolation

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The rice-artificial sea water culture (10 kg) of F. solani H915 was extracted with ethyl acetate

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(EtOAc, 20 L) three times and concentrated under reduced-pressure at 40 ºC to yield 16.4 g crude

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extract. Then, 15.0 g of the EtOAc extract was divided into 10 fractions (Fr. 1 – Fr. 10) over a silica

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gel (300 g) column eluted with petroleum ether : ethyl acetate (v/v) (20:1; 10:1; 5:1; 2:1; 1:1, 1.0 L

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each) and chloroform : methyl alcohol (v/v) (50:1; 20:1; 10:1; 5;1; 2:1; 0:1, 1.0 L each). Except for the

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low-yielding non-polar (Fr. 1 – Fr. 4) and extreme high-polar fractions (Fr. 10), most of the fractions

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were subjected to ODS columns for further separation. Fr. 5 (1.1 g) was separated over 20 g ODS and

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eluted with 10%, 30%, 50%, 70% and 100% CH3OH/H2O (0.2 L each) to give five subfractions (subFr.

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5.1 – subFr. 5.5). SubFr. 5.5 was purified by preparative HPLC ODS column and isocratic eluted with

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acetonitrile-H2O (27:73, v/v) to yield compounds 9 (15 mg) and 10 (15 mg). Fr. 6 (4.6 g) was subjected

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to an ODS (100 g) column and eluted with 10%, 30%, 50%, 70% and 100% CH3OH/H2O (0.5 L each)

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to give nine subfractions (subFr. 6.1 – subFr. 6.9). Then, subFr. 6.9 (1.3 g) was purified by preparative

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HPLC and isocratic eluted with acetonitrile-H2O (42:58, v/v) to give compounds 1 (7 mg), 2 (7 mg)

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and 7 (300 mg). Fractionation of Fr. 7 (1.3 g), resulting in 8 subfractions (subFr. 7.1 – subFr. 7.8), was

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conducted via an ODS (30 g) column eluted with 10%, 30%, 50%, 70% and 100% CH3OH/H2O (0.3

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L each). SubFr. 7.8 (80 mg) was purified by preparative HPLC, isocratic eluted with acetonitrile-H2O

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(45:55, v/v), to afford compounds 3 (10 mg) and 5 (10 mg). Fr. 8 (1.5 g) was separated using an ODS

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(30 g) column, eluted with CH3OH/H2O (10% – 100%, 0.3 L each), to give a 10 subfractions (subFr.

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8.1 – subFr. 8.10). Preparative HPLC with isocratic elution of acetonitrile : H2O (44:56, v/v) was used

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for subFr. 8.7 (105 mg), leading to the isolation of compound 6 (10 mg). Fr. 9 (3.8 g) was also separated

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using an ODS (100 g) column, eluting with 0%, 30%, 50%, 70% and 100% CH3OH/H2O (0.5 L each),

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to give nine subfractions (subFr. 9.1 – subFr. 9.9). SubFr. 9.7 (325 mg) was purified by preparative

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HPLC isocratic eluted with acetonitrile-H2O (45:55, v/v) to obtain compounds 4 (6 mg), 8 (8 mg), 11

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(14 mg) and 12 (19 mg).

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Fusarilactone A (1)

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Isolated as a white amorphous powder; HRESIMS m/z 345.1668 [M+Na]+ (calcd. for C18H26O5Na,

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345.1672) in the positive mode, and m/z 321.1711 [M-H]- (calcd. for 321.1707 C18H25O5) in the

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negative mode; [α]D25 = +16° (c = 0.5, CH3OH), IR (KBr) (νmax): 3406, 1654, 1436 cm−1. UV (CH3OH)

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λmax (log ε): 201 (3.91), 233 (3.58), 266 (3.80) nm. 1H NMR (600 MHz, DMSO-d6) and 13C NMR (150

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MHz, DMSO-d6) data are listed in Table 1 and Table 2.

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Fusarilactone B (2)

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Isolated as a white amorphous powder; HRESIMS m/z 333.1671 [M+Na]+ (calcd. for C17H26O5Na,

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333.1672) in the positive mode, and m/z 309.1707 [M-H]- (calcd. for 309.1707 C17H25O5) in the

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negative mode; [α]D25 = 0° (c = 0.5, CH3OH). UV (CH3OH) λmax (log ε): 216 (4.38), 267 (4.03) nm.

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IR (KBr) (νmax): 3385, 1646, 1436 cm−1. 1H NMR (600 MHz, DMSO-d6) and 13C NMR (150 MHz,

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DMSO-d6) data are listed in Table 1 and Table 2.

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Fusarilactone C (3)

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Isolated as a white amorphous powder; HRESIMS m/z 375.2140 [M+Na]+ (calcd. for C20H32O5Na,

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375.2142) in the positive mode, and m/z 351.2179 [M-H]- (calcd. for 351.2177 C20H31O5) in the

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negative mode; [α]D25 = 2° (c = 0.5, CH3OH). UV (CH3OH) λmax (log ε): 228 (3.53) and 266 (3.85)

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nm. IR (KBr) (νmax): 3406, 2927, 1560, 1430 cm−1. 1H NMR (600 MHz, DMSO-d6) and 13C NMR (150

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MHz, DMSO-d6) data are listed in Table 1 and Table 2.

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Fusaridioic acid B (4)

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Isolated as a white amorphous powder; HRESIMS m/z 351.1768 [M+Na]+ (calcd. for C17H28O6Na,

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351.1778) in the positive mode, and m/z 327.1806 [M-H]- (calcd. for 327.1813 C17H27O6) in the

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negative mode; [α]D25 = 0° (c = 0.5, CH3OH), IR (KBr) νmax): 3406, 1570, 1430 cm−1. UV (CH3OH)

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λmax (log ε): 231 (3.57), 266 (3.86) nm. 1H NMR (600 MHz, DMSO-d6) and

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DMSO-d6) data are listed in Table 1 and Table 2.

13C

NMR (150 MHz,

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Fusaridioic acid C (5)

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Isolated as a white amorphous powder; HRESIMS m/z 349.1985 [M+Na]+ (calcd. for C18H30O5Na,

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349.1985) in the positive mode, and m/z 325.2030 [M-H]- (calcd. for 325.2020 C18H29O5) in the

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negative mode; [α]D25 = 0° (c = 0.5, CH3OH), IR (KBr) (νmax): 3416, 1570, 1430 cm−1. UV (CH3OH)

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λmax (log ε): 229 (3.47), 268 (3.93) nm. 1H NMR (600 MHz, DMSO-d6) and

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DMSO-d6) data are listed in Table 1 and Table 2.

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Antifungal activity assay

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13C

NMR (150 MHz,

The antifungal assay of all the isolated compounds was performed against tea pathogenic fungus 30

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P. theae using a previously described method.

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which a 0.6 cm diameter piece of tested fungal strains cylinder agar was placed on the center and sterile

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blank paper discs of 0.5 cm diameter were placed at a distance of 2 cm away from the growing mycelial

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colony. Approximately 20 μg compound was added to each paper disc. DMSO without compound was

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used as the blank control and commercial fungicide hexaconazole was used as the positive control.

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The plates were incubated at 28 °C until mycelial growth covered the control discs.

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ED50 Detection.

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As reported previously,

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The tests were carried out in PDA Petri plates in

compounds with final concentrations of 150–4.688 µg/mL (two-fold

dilution) were mixed with PDA medium and poured into a set of PDA Petri plates. P. theae mycelial

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cylinder agar (0.6 cm) was placed in the center of each treated Petri dish. Treated Petri dishes were

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then incubated at 28°C until the fungal growth covered the blank control plates. DMSO was used as

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the blank control and hexaconazole was used as the positive control. Mycelial growth of fungus (cm)

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in both treated (T) and control (C) were measured diametrically. The mean and standard deviation

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were calculated to determine the percentage inhibition of growth (I%) with the formula

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− T)/C × 100. Corrected inhibition (%) = [(% I − C.F.)/(100 − C.F.)] × 100. Correction

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Factor (CF) = [(90 − C)/C] × 100 as described previously. 31 From the concentration (μg/mL) and

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corresponding corrected percent growth inhibition data, the ED50 (μg/mL) value was calculated

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statistically by Probit analysis, using the Probit Package of MSTATC software. The experiment was

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repeated three times.

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Total RNA Isolation

I (%) = (C

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P. theae cells cultured in PDA medium for 3 days at 28°C were treated with compounds 1–12 at

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10 µg/mL for 16 h. Cells were harvested by centrifuging at 6000 rpm for 5 min. Cells were then

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homogenized in liquid nitrogen and total RNA was extracted with Spin Column Fungal Total RNA

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Purification Kit (Sangon Biotech, China) and stored at -80 °C until use.

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Real-Time RT-PCR Analysis of HMG-CoA synthase expression

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The effect of compounds 1–12 on the mRNA expression of HMG-CoA synthase of P. theae cells

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at a concentration of 10 µg/mL was analyzed by Real-Time PCR. DMSO and abscisic acid at 10 µg/mL

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32

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HMG-CoA synthase (forward: TACTCGCTCACCTGCTACAC, reverse: GCGTACGACTTCTG

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GACGAC), and GAPDH (forward: CATGTCCATGCGTGTCCCTA, reverse: CAGTGGAGA

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CAACCTCGTCC) was determined by real-time RT-PCR. The cDNA was synthesized from total RNA

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using PrimeScript RT reagent kit with gDNA Eraser (Takara, Japan). TaKaRa SYBR® Premix Ex

were used as the blank and positive controls, respectively. The expression of mRNA transcripts of

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Taq™ II (Takara, Japan) and Stepone Real-Time PCR Detection System (Applied Biosystems, USA)

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were used for Real-Time PCR analysis.

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RESULTS AND DISCUSSION

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Structural Elucidation

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A series of chromatographic methods was used during the isolation of an extract from the rice-artificial

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sea water medium of F. solani H915. As a result, 12 compounds were isolated, including five new

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compounds, fusarilactone A (1), fusarilactone B (2), fusarilactone C (3), fusaridioic acid B (4),

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fusaridioic acid C (5), and seven known compounds (6–12, Figure 1).

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Insert Figure 1 here

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Compound 1 (fusarilactone A) was isolated as an amorphous white powder. The molecular

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formula C18H26O5, which gave six degrees of unsaturation, was established by positive and negative

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HRESIMS ion peaks at m/z 345.1668 [M+Na]+ and 321.1711 [M-H]-, respectively. The UV maximum

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absorption bands at max (log ε): 233 (3.58) nm and 266 (3.80) were assigned to an unconjugated

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carbonyl and a conjugated carbonyl, respectively. In the low-field region of the 13C NMR, two carboxyl

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carbon signals were observed at δC 170.8 (C-14) and δC 168.6 (C-1, which is conjugated with the

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double bond system). In the low field region of the 1H NMR, two olefinic protons were observed with

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br. s peaks at δH 5.55 (H-2) and 5.71 (H-4). In addition, a pair of trans-alkene hydrogens were observed

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at δH 5.34 (H-8) and 5.40 (H-9), with a coupling constant of 15.4 Hz. Their corresponding olefinic

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carbon signals were found in the sp2 region of the 13C NMR spectrum at δC 119.2 (C-2), 152.4 (C-3),

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129.9 (C-4), 140.4 (C-5), 136.8 (C-8), and 127.1 (C-9). The sp3 high-field region of the 1H NMR

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spectrum showed three methyl proton signals. Two br.s methyl peaks at δH 2.13 (3-CH3) and 1.76 (5-

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CH3) were assigned as being linked to quaternary olefinic carbons. The other methyl at δH 0.91 (d, J =

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6.6 Hz, 7-CH3) is connected to a methylene group. In the 1H-1H COSY spectrum, the proton at δH 4.52

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(m) showed correlations with those at δH (1.85 & 1.78, H-11) and δH 3.51 (H-13). The HSQC spectrum,

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signal at δH 4.52 (m) showed a correlation with the carbon at δC 74.3, which was attributed to the 12-

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CH. The two dd peaks at δH 3.71 (J = 11.6, 3.9 Hz) and 3.61 (J = 11.6, 3.5 Hz), which form a typical

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ABX coupling system with H-13 (δH 3.51), were assigned to the two protons at 13-CH2OH.

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Elucidation of HSQC, 1H-1H COSY and HMBC spectra indicated that the planar structure of 1 was

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almost identical to that of hymeglusin (7), 33-34 a typical β-lactone antibiotic isolated from a culture of

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Scopulariopsis sp. F-244. Most of the 1D NMR spectral data of 1 were very similar to those of

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hymeglusin (7), except for the signals at positions 8 and 9. The molecular formula of 1 has two fewer

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hydrogen atoms than 7, and the pair of olefinic 1H and 13C signals that appeared in the sp2 low field

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region, indicated that 1 was the dehydrogenation product of hymeglusin (7) at positions 8 and 9. The

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configuration of the double bonds was also revealed by a NOESY experiment, where correlations

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between H-4 (δH 5.71) and H-2 (δH 5.55), as well as H-4 and H-6 (δH 2.02, 1.97), were observed.

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Moreover, the NOESY correlation between H-12 and 13-CH2OH indicated the trans-configuration of

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C-11 and 13-CH2OH, which was confirmed by the 1H and 13C NMR similarity of C-11, C-12, C-13

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and C-14 between compounds 1 and 7. As a result, the structure of 1 was elucidated and called as

239

fusarilactone A (Figure 2).

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The theoretical electronic circular dichroism (ECD) spectra of 7S, 12R, 13R -1 and 7R, 12R, 13R -1

241

were also calculated and compared with the experimental values to determine the absolute

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configuration. As shown in Figure 3a, the experimental ECD spectrum was similar to both of the

243

calculated ECD spectra. Considering that the absolute configuration of C-7 cannot be determined by

244

the CD spectrum and ECD calculation, since carbon chain flexibility. The absolute configuration of 1

245

was determined to be 12R, 13R (Figure 3).

246 247

Insert Table 1 here

248 249

Insert Table 2 here

250 251

Insert Figure 2 here

252 253

Insert Figure 3 here

254 255

Compound 2 (fusarilactone B) was obtained as an amorphous white powder. Positive and

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negative HRESIMS suggested its molecular formula to be C17H26O5, which gave five degrees of

257

unsaturation. The 1H, 13C NMR and DEPT spectra, in which two carbonyls, two ethylenic bonds, two

258

methyls and seven methylenes were found, indicated that the structure of 2 was similar to that of

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hymeglusin (7), except for the absence of a methyl at C-7. In the 13C NMR of 2, the carbon signal at δ

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19.5 assigned to 7-CH3 of hymeglusin (7) was not present. The 13C chemical shift of C-7 changed from

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δC 30.8 to 28.9, and the 13C NMR signals assigned to C-6 and C-8 were also shifted from δC 48.8 to

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40.7 and from δC 36.6 to 28.9, respectively. The structure of 2, including its relative configuration, was

263

further supported by the correlations found in 1H-1H COSY, HMBC and NOESY spectra (Figure 2).

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Compound 3 (fusarilactone C) was isolated as an amorphous white powder. The molecular

265

formula of C20H32O5, suggested by positive and negative HRESIMS, gave five degrees of unsaturation.

266

Two carbonyl carbons and two ethylenic bonds could be found in the low field region of the 13C NMR.

267

The high field region of the 1H, 13C NMR and DEPT spectra showed two oxygenated methines, three

268

methines without an oxygen-link, six methylenes and four methyls. Compound 3 also has a similar

269

structure to hymeglusin (7). However, the 1H-1H COSY correlations of H-12/H-13, H-13/H-14 and H-

270

14/H-15, 15-CH3, together with the HMBC correlations from H-12 to C-16, and from H-15 to C-16,

271

supported the presence of a six-membered lactone ring in 3, which is different from the β-lactone ring

272

in compound 1, 2 and 7. The relative configuration of 3 was further assigned using NOESY

273

correlations between H-12 (δH 4.55) and H-14 (δH 3.94), H-13e (δH 1.91), and between H-14 and H-

274

13e, 15-CH3 (δH 1.10), as well as the correlations induced by the protons at the opposite side of the six-

275

membered ring: between H-15 (δH 2.50) and H-13a (δH 1.70). The configuration of the double bonds

276

was similar with those in compounds 1 and 2, as shown in Figure 2.

277

Compound 4 (fusaridioic acid B) was isolated as an amorphous white powder with positive and

278

negative HRESIMS ion peaks at m/z 351.1768 [M+Na]+, and 327.1806 [M-H]-, respectively. Its

279

molecular weight is higher than that of 2 by 18 mass units, indicating that 4 could be the ring-opened

280

analog of 2. Most of the 1H, 13C NMR and DEPT data of 4 were similar to those of 2, except for the

281

carbon signals assigned to C-12, 13 and 14. In detail, the chemical shift of the 14-carbonyl was shifted

282

from δC 170.9 to 175.4, while that of C-12 was shifted from δC 74.8 to 69.3. 1H-1H COSY, HMBC and

283

NOESY spectra helped to confirm the structure of 4 as the ring-opened analog product of 2. As

284

described in a previous report 35, hydrogen bonds between the C-14 carbonyl oxygen and 12-OH, and

285

between the 14-carboxyl hydroxyl and 13-CH2OH helped to elucidate the relative configuration

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286

between C-12 and C-13 by using the coupling constant between H-12 and H-13. In compound 4, the

287

trans-coplanar position induced an H12-H13 coupling constant of 7–8 Hz (Figure 2). Thus, the coupling

288

splitting of H-13 in 4 presented as a td peak with J values of 7.0 Hz (× 2) and 4.6 Hz (× 1) (Table 1).

289

Compound 5 (fusaridioic acid C) was also isolated as an amorphous white powder. The molecular

290

formula of C18H30O6, was established by the HRESIMS positive and negative ion peaks at m/z

291

349.1985 [M+Na]+ and 325.2030 [M-H]-. The

292

carbons and four olefinic carbons belonging to two ethylenic bonds, in the sp2 low field region. The

293

sp3 high field region of the 1H NMR and 13C NMR spectra showed the existence of two methine, five

294

methylenes and four methyls. The 13C NMR data of the C-14 carbonyl was closer to that of compound

295

4, rather than compounds 1 and 2, indicating that compound 5 was also a ring-opened structure.

296

Comparing the NMR data with those of 4, signals of a methylene bearing an oxygen [(δC 60.3 and δH

297

3.49, 3.45), 13-CH2OH] disappeared and a set of methyl signal emerged at [δC 13.1 and δH 0.98 (3H,

298

d, J = 8.3 Hz), 11-CH3]. The 1H-1H COSY, HMBC and NOESY spectra confirmed the loss of the 13-

299

CH2OH and presence of the methyl at C-11, and the structure of 5 was elucidated as shown in Figure

300

2.

13C

NMR and DEPT spectra, showed two carbonyl

301

The known compounds 6–12 were characterized by HRESIMS and NMR spectra. Only the

302

NMR data of hymeglusin (7) 33-34, and L-660282 (11), 36-37 had been reported previously, and they were

303

identified by comparison with the known NMR data. Fusaridioic acid A (12) was a new compound,

304

recently reported by our group.

305

using 2D-NMR and identified as: (2E,4E,7S)-12,14-dihydroxy-3,5,7-trimethyl-tetradeca-2,4-dienoic

306

acid

307

trienedioic acid (8), (2E,5E)-3,5,7-trimethylocta-2,5-dienedioic acid (9), and (2E,4E)-3,5,7-

(6),

35

Chemical structures of other known compounds were elucidated

(2E,4E,7S,8E,12S,13S)-12-hydroxy-13-(hydroxymethyl)-3,5,7-trimethyltetradeca-2,4,8-

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308

trimethylocta-2,4-dienedioic acid (10). Their 1H-NMR data were given in Table S1 of the supporting

309

information, and their 13C-NMR data were listed in Table 2.

310 311

Evaluation of antifungal activity

312

The antifungal activity of the isolated compounds was evaluated using a paper disc inhibition

313

assay and ED50 detection. Compounds 1, 2, and 7 demonstrated significant activities against the tea

314

pathogenic fungus P. theae (Figure 4), however, none of the other compounds exhibited this effect.

315

The ED50 values of new compounds 1 and 2 and known compound 7 were 38.14 ± 1.67 µg/mL, 42.26

316

± 1.96 µg/ mL, 18.35 ± 1.27 µg/ mL to P. theae respectively, while the positive control hexaconazole

317

was 16.34 ± 1.25 µg/ mL. All 12 compounds were also tested for cytotoxic effects against mice 3T3-

318

L1 cells through CCK8 methods, but they did not show significant cytotoxicity (IC 50 > 100 µM).

319 320

Insert Figure 4 here

321 322

Concerning the structure-activity relationship, the results showed that the β-lactone ring seemed

323

to be important for the anti-fungal inhibitory activity. Compounds 1, 2, and 7, which contain a β-

324

lactone ring, showed potent activity, while their corresponding open ring derivatives, compounds 8, 4,

325

11, and 12, showed no inhibitory activity against P. theae. The number of double bonds, and the methyl

326

side chain did not influence the activity. The fact that compound 9 and 10 did not show activity

327

indicated that the long main aliphatic chain may be necessary for the anti-fungal activity.

328

The most potent compounds in this study were hymeglusin (7) and its derivatives fusarilactone A

329

(1) and fusarilactone B (2). Hymeglusin (7) was reported as a 3-hydroxy-3-methylglutaryl-CoA

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(HMG-CoA) synthase specific inhibitor by covalently binding through the β-lactone to the active Cys

331

129 residue of the enzyme

332

distributed in eukaryotes (vertebrates, insects, plants and fungi), archaea, and certain bacteria 40-41 and

333

takes part in three metabolic pathways: synthesis and degradation of ketone bodies, valine, leucine and

334

isoleucine degradation, and butanoate metabolism.

335

potential for antiviral, 42-43 anti-bacterial, 39 cardiovascular protection. 44-45

336

38-39

and forming of a thioester adduct. HMG-CoA synthase is widely

42

Inhibition of HMG-CoA synthase has shown

It is known that fungisterol, mainly refers to ergosterol, is an important and specific component 46.

337

of the fungal cell membrane

338

mevalonate biosynthesis, farnesyl-PP biosynthesis and ergosterol biosynthesis. HMG-CoA synthase

339

(ERG13) is the second key enzyme that catalyzes the condensation of a third acetyl-CoA to

340

acetoacetyl-CoA to yield 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) during mevalonate

341

biosynthesis

342

cubense tropical race4 (Foc TR4), an important lethal pathogen of bananas, both the mRNA and the

343

protein of HMG-CoA synthase were significantly upregulated.

344

potential new target for the effective inhibition of Foc R4 early growth for controlling Fusarium wilt

345

of bananas. A previous report indicated that statins which target HMG-CoA reductase, the third key

346

enzyme of mevalonate biosynthesis, can strongly inhibit the growth of the Candida species and

347

Aspergillus fumigatus, while these effects could be reversed by supplementation of the

348

culture with ergosterol.

349

potent inhibitory activity against tea pathogenic fungus P. theae and significant HMG-CoA synthase

350

gene expression downregulation (Figure 5). These results implied that inhibition of HMG-CoA

351

synthase gene expression by compounds 1, 2 and 7 could inhibit the growth of tea pathogenic fungi

47.

The biosynthesis of ergosterol can be divided into three parts:

It was reported that during the conidial germination of Fusarium oxysporum f. sp.

49

48

This protein is thought to be a

In our tests, the new compounds 1, 2 and known compound 7 exhibited

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352

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by interrupting ergosterol biosynthesis.

353

Insert Figure 5 here

354 355 356

Acknowledgements

357

The project was supported by the COMRA Project of China (DY135-B2-16), National Basic

358

Research Program of China (973 Program) (No. 2015CB755901), the Xiamen Ocean Economic

359

Innovation and Development Demonstration Project (16PZP001SF16), Fujian Key Science and

360

Technology Program (No.2018N0017), Scientific Research Foundation of Third Institute of

361

Oceanography, SOA. (No.2016002, 2017002) and Xiamen Science and Technology Program

362

(No.3502Z20172009 and 3502Z20182029).

363

References

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Saha, D.; Dasgupta, S.; Saha, A., Antifungal activity of some plant extracts against fungal pathogens of tea (Camellia

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Gunasekera, T.; Paul, N.; Ayres, P., The effects of ultraviolet-B (UV-B: 290-320 nm) radiation on blister blight disease

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bacterium of Bacillus subtilis isolated from tea leaves. Plant Pathol J 2009, 25, 99-102. 10. Naglot, A.; Goswami, S.; Rahman, I.; Shrimali, D.; Yadav, K. K.; Gupta, V. K.; Rabha, A. J.; Gogoi, H.; Veer, V., Antagonistic potential of native Trichoderma viride strain against potent tea fungal pathogens in North East India. The

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11. Sowndhararajan, K.; Marimuthu, S.; Manian, S., Biocontrol potential of phylloplane bacterium Ochrobactrum 12. Elango, V.; Manjukarunambika, K.; Ponmurugan, P.; Marimuthu, S., Evaluation of Streptomyces spp. for effective management of Poria hypolateritia causing red root-rot disease in tea plants. Biological Control 2015, 89, 75-83. 13. Marrone, P. G., Barriers to adoption of biological control agents and biological pesticides. Integrated pest management. Cambridge University Press, Cambridge 2009, 163-178. 14. Yoon, M.-Y.; Kim, Y. S.; Ryu, S. Y.; Choi, G. J.; Choi, Y. H.; Jang, K. S.; Cha, B.; Han, S.-S.; Kim, J.-C., In vitro and in vivo antifungal activities of decursin and decursinol angelate isolated from Angelica gigas against Magnaporthe oryzae, the causal agent of rice blast. Pesticide biochemistry and physiology 2011, 101 (2), 118-124. 15. Xiao, J.; Zhang, Q.; Gao, Y. Q.; Tang, J. J.; Zhang, A. L.; Gao, J. M., Secondary metabolites from the endophytic Botryosphaeria dothidea of Melia azedarach and their antifungal, antibacterial, antioxidant, and cytotoxic activities. J Agric Food Chem 2014, 62 (16), 3584-90. 16. Su, H.; Wang, W.; Bao, L.; Wang, S.; Cao, X., Synthesis and Evaluation of Essential Oil-Derived β-Methoxyacrylate Derivatives as High Potential Fungicides. Molecules 2017, 22 (5), 763. 17. Zhang, Z.-Z.; Li, Y.-B.; Qi, L.; Wan, X.-C., Antifungal activities of major tea leaf volatile constituents toward Colletorichum camelliae Massea. Journal of agricultural and food chemistry 2006, 54 (11), 3936-3940. 18. Kiuru, P.; DʼAuria, M. V.; Muller, C. D.; Tammela, P.; Vuorela, H.; Yli-Kauhaluoma, J., Exploring marine resources for bioactive compounds. Planta medica 2014, 80 (14), 1234-1246. 19. Gerwick, W. H.; Fenner, A. M., Drug discovery from marine microbes. Microbial ecology 2013, 65 (4), 800-806. 20. Li, W.; Tang, X.-X.; Yan, X.; Wu, Z.; Yi, Z.-W.; Fang, M.-J.; Su, X.; Qiu, Y.-K., A new macrolactin antibiotic from deep sea-derived bacteria Bacillus subtilis B5. Natural product research 2016, 30 (24), 2777-2782. 21. Alongi, D. M., Carbon cycling and storage in mangrove forests. Ann Rev Mar Sci 2014, 6, 195-219. 22. Chen, Q.; Zhao, Q.; Li, J.; Jian, S.; Ren, H., Mangrove succession enriches the sediment microbial community in South China. Sci Rep 2016, 6, 27468. 23. Wang, K. W.; Wang, S. W.; Wu, B.; Wei, J. G., Bioactive natural compounds from the mangrove endophytic fungi. Mini Rev Med Chem 2014, 14 (4), 370-91. 24. Xu, D. B.; Ye, W. W.; Han, Y.; Deng, Z. X.; Hong, K., Natural products from mangrove actinomycetes. Mar Drugs 2014, 12 (5), 2590-613. 25. Li, G.; Kusari, S.; Golz, C.; Strohmann, C.; Spiteller, M., Three cyclic pentapeptides and a cyclic lipopeptide produced by endophytic Fusarium decemcellulare LG53. RSC Advances 2016, 6 (59), 54092-54098. 26. Yang, S. X.; Gao, J. M.; Laatsch, H.; Tian, J. M.; Pescitelli, G., Absolute configuration of fusarone, a new azaphilone from the endophytic fungus Fusarium sp. isolated from Melia azedarach, and of related azaphilones. Chirality 2012, 24 (8), 621-7. 27. Yang, S. X.; Gao, J. M.; Zhang, Q.; Laatsch, H., Toxic polyketides produced by Fusarium sp., an endophytic fungus isolated from Melia azedarach. Bioorg Med Chem Lett 2011, 21 (6), 1887-9. 28. Yang, S. X.; Wang, H. P.; Gao, J. M.; Zhang, Q.; Laatsch, H.; Kuang, Y., Fusaroside, a unique glycolipid from Fusarium sp., an endophytic fungus isolated from Melia azedarach. Org Biomol Chem 2012, 10 (4), 819-24. 29. Yang, S.-X.; Xiao, J.; Laatsch, H.; Holstein, J. J.; Dittrich, B.; Zhang, Q.; Gao, J.-M., Fusarimine, a novel polyketide isoquinoline alkaloid, from the endophytic fungus Fusarium sp. LN12, isolated from Melia azedarach. Tetrahedron Letters 2012, 53 (47), 6372-6375. 30. Woo, J.-H.; Kitamura, E.; Myouga, H.; Kamei, Y., An antifungal protein from the marine bacterium Streptomyces sp. strain AP77 is specific for Pythium porphyrae, a causative agent of red rot disease in Porphyra spp. Applied and environmental microbiology 2002, 68 (6), 2666-2675.

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31. Kundu, A.; Saha, S.; Walia, S.; Shakil, N. A.; Kumar, J.; Annapurna, K., Cadinene sesquiterpenes from Eupatorium adenophorum and their antifungal activity. Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes 2013, 48 (6), 516-22. 32. Alex, D.; Bach, T. J.; Chye, M.-L., Expression of Brassica juncea 3-hydroxy-3-methylglutaryl CoA synthase is developmentally regulated and stress-responsive. The Plant Journal 2001, 22 (5), 415-426. 33. Tomoda, H.; Kumagai, H.; Takahashi, Y.; Tanaka, Y.; Iwai, Y.; Omura, S., F-244 (1233A), a specific inhibitor of 3hydroxy-3-methylglutaryl coenzyme A synthase: taxonomy of producing strain, fermentation, isolation and biological properties. J. Antibiot. 1988, 41 (2), 247-9. 34. Kumagai, H.; Tomoda, H.; Omura, S., Biosynthesis of antibiotic 1233A (F-244) and preparation of [14C]1233A. J. Antibiot. 1992, 45 (4), 563-7. 35. Liu, S. Z.; Yan, X.; Tang, X. X.; Lin, J. G.; Qiu, Y. K., New Bis-Alkenoic Acid Derivatives from a Marine-Derived Fungus Fusarium solani H915. Mar Drugs 2018, 16 (12), 483. 36. Greenspan, M. D.; Yudkovitz, J. B.; Lo, C. Y. L.; Chen, J. S.; Alberts, A. W.; Hunt, V. M.; Chang, M. N.; Yang, S. S.; Thompson, K. L.; et, a., Inhibition of hydroxymethylglutaryl-coenzyme A synthase by L-659,699. Proc. Natl. Acad. Sci. U. S. A. 1987, 84 (21), 7488-92. 37. Aldridge, D. C.; Giles, D.; Turner, W. B., Antibiotic 1233A, a fungal β-lactone. J. Chem. Soc. C 1971,

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38. Tomoda, H.; Ohbayashi, N.; Morikawa, Y.; Kumagai, H.; Ōmura, S., Binding site for fungal β-lactone hymeglusin on cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids 2004, 1636 (1), 22-28. 39. Skaff, D. A.; Ramyar, K. X.; McWhorter, W. J.; Barta, M. L.; Geisbrecht, B. V.; Miziorko, H. M., Biochemical and structural basis for inhibition of Enterococcus faecalis hydroxymethylglutaryl-CoA synthase, mvaS, by hymeglusin. Biochemistry 2012, 51 (23), 4713-4722. 40. Bahnson, B. J., An atomic-resolution mechanism of 3-hydroxy-3-methylglutaryl–CoA synthase. Proceedings of the National Academy of Sciences of the United States of America 2004, 101 (47), 16399-16400. 41. Bearfield, J.; Keeling, C.; Young, S.; Blomquist, G.; Tittiger, C., Isolation, endocrine regulation and mRNA distribution of the 3‐hydroxy‐3‐methylglutaryl coenzyme A synthase (HMG‐S) gene from the pine engraver, Ips pini (Coleoptera: Scolytidae). Insect molecular biology 2006, 15 (2), 187-195. 42. Liao, P.; Wang, H.; Hemmerlin, A.; Nagegowda, D. A.; Bach, T. J.; Wang, M.; Chye, M.-L., Past achievements, current status and future perspectives of studies on 3-hydroxy-3-methylglutaryl-CoA synthase (HMGS) in the mevalonate (MVA) pathway. Plant cell reports 2014, 33 (7), 1005-1022. 43. Peng, L. F.; Schaefer, E. A.; Maloof, N.; Skaff, A.; Berical, A.; Belon, C. A.; Heck, J. A.; Lin, W.; Frick, D. N.; Allen, T. M. J. J. o. I. D., Ceestatin, a novel small molecule inhibitor of hepatitis C virus replication, inhibits 3-hydroxy-3-methylglutarylcoenzyme A synthase. 2011, 204 (4), 609-616. 44. HEGARDT, F. G., Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase: a control enzyme in ketogenesis. Biochemical Journal 1999, 338 (3), 569-582. 45. Greenspan, M. D.; Bull, H.; Yudkovitz, J.; Hanf, D. P.; Alberts, A. W., Inhibition of 3-hydroxy-3-methylglutaryl-CoA synthase and cholesterol biosynthesis by β-lactone inhibitors and binding of these inhibitors to the enzyme. Biochemical Journal 1993, 289 (3), 889-895. 46. Prasad, R.; Shah, A. H.; Rawal, M. K. J. O. T. t. T. X., Antifungals: Mechanism of Action and Drug Resistance. 2016, 892, 327-349. 47. Hu, Z.; He, B.; Ma, L.; Sun, Y.; Niu, Y.; Zeng, B. J. I. J. o. M., Recent Advances in Ergosterol Biosynthesis and Regulation Mechanisms in Saccharomyces cerevisiae. 2017, 57 (3), 270. 48. Deng, G. M.; Yang, Q. S.; He, W. D.; Li, C. Y.; Yang, J.; Zuo, C. W.; Gao, J.; Sheng, O.; Lu, S. Y.; Zhang, S. J. A. M.; Biotechnology, Proteomic analysis of conidia germination in Fusarium oxysporum f. sp. cubense tropical race 4 reveals

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new targets in ergosterol biosynthesis pathway for controlling Fusarium wilt of banana. 2015, 99 (17), 7189-7207. 49. IG, M.; G, J.; T, S.; Letters, M. P. J. F. M., Growth inhibition of Candida species and Aspergillus fumigatus by statins. 2010, 262 (1), 9-13.

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Figure Legends

478 479

Figure 1. Structures of the isolated alkenoic acids from Fusarium solani H915.

480 481

Figure 2. Key 1H-1H COSY, HMBC and NOESY correlations of the new alkenoic acids.

482 483

Figure 3. Calculated and experimental electronic circular dichroism (ECD) spectra of the compound

484

1.

485 486

Figure 4. Inhibitory activity of compounds 1, 2, and 7 against tea pathogenic fungus Pestalotiopsis

487

theae.

488 489

Figure 5. Regulation effects of compounds 1–12 on the mRNA expression of HMG-CoA synthase.

490

The gene expression level was determined by real-time RT-PCR. DMSO (B) and abscisic acid (P)

491

were used as the blank and positive controls, respectively. GAPDH was used as reference gene.

492

Values represent the mean ± SD of three independent experiments. ***P < 0.001.

493

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495

Table Captions

496 497

Table 1. 1H NMR data (600 MHz, DMSO-d6) of compounds 1–5.

498 499

Table 2. 13C NMR data (150 MHz, DMSO-d6) of compounds 1–12.

500 501

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Figures

502

9

HOH2C 13 12

14

5

8

O

O

7

1

3 4

COOH 2

HOH2C 13 14

O

5 12

4

O

HOOC

2OH

5

1

3 4

OH

COOH 2

HOOC

14

7

12

14

O

5

12

4

O

1

3

COOH 2

13

14

CH2OH 12

OH

7

5

HOOC 8

503 504

6

4

1

3 4

COOH 2

14

HOH2C

13

7

1 2

9

7

1

3

COOH

13 CH

HOOC

14

2OH

COOH 2

5

1

3 4

12

4

COOH 2

7

COOH

5

HOOC

2

1

3

COOH

4

8

2

9

5

1

3 4

OH

1

3

6

8

7

5

OH

8

2

10

5

11

HOOC

1

3

O

5

7

7

16

15

5

3

OH

4

HOH2C 13

14

7

1 2

2

12

14

COOH 2

13

HO

O

1

13 CH

1

3

COOH 2

11

Figure 1

505

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13 CH

HOOC

14

2OH

7

12

5 4

OH 12

1

3

COOH 2

Journal of Agricultural and Food Chemistry

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506

7

HOH2C

13

9

12

O 14

O

3

5

1

3

HOH2C

COOH

13

8

O 14

507 508

12

COOH

3 5

1

COOH

OH

HOH2C 11

H12 14 COOH 13 N OH H13

5

HO 14 15

12 1 6

O

O

2

CH2OH 14

5

12

1

O

1

HOOC 13

NOESY

HMBC

COSY

3

7

HOOC 14

12

OH 5

4

Figure 2

509 510

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5

3

1

COOH

3

1

COOH

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Expt. Compd. 1 Calcd. 7S, 12R, 13R-1 Calcd. 7R, 12R, 13R-1

511 512

Figure 3

513 514 515 516

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517

518 519

Figure 4

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520 521

Figure 5

522 523

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524

Tables

525

Table 1. Position

1

2

3

Page 28 of 30

4

5

7

2

5.55 br. s

5.56 br. s

5.57 br. s

5.51 br. s

5.58 br. s

5.57 br. s

4

5.71 br. s

5.74 br. s

5.73 br. s

5.68 br. s

5.73 br. s

5.73 br. s

2.06 dd (13.0,

2.07 dd (13.2, 6.1)

6a 6b

2.02 dd (12.8, 8.0) 1.98 dd (12.8, 2.33 spt (6.7)

8a

5.34 dd (15.4,

8b

6.8)

9a

5.40 dt (15.4,

9b

6.2)

10b 11a 11b 12 13a 13b

2.05 br. t (7.4)

7.5)

7

10a

2.07 dd (12.9,

2.04 m 1.85 dt (14.0, 6.9) 1.78 dt (14.0, 6.8) 4.52 m

6.0)

1.98 br. t

6.2)

1.83 dd (12.9,

(7.4)

1.83 dd (13.0,

8.5) 1.26 m

1.65 m 1.24 m

1.24 m

1.08 m

1.41 m

1.28 m

1.33 m

1.34 m

1.79 m

1.60 m

8.2) 1.33 m 1.17 m 1.17 m

1.81 m

1.64 m

1.65 m

1.26 m

1.27 m

1.07 m

1.10 m

1.37 m 1.26 m

1.32 m

1.33 m

1.35 m

1.37 m

1.18 m

1.27 m

1.29 m

1.32 m

1.81 m 2.34 m

1.73 m 4.53 td (6.7, 4.2)

1.52 m

1.23 m

4.55 m

3.53 m

1.74 m 3.57 br. t

4.53 td (6.7,

(5.9)

4.3)

1.91 dt (13.7,

3.51, covered by

3.51 br. dd

3.5)

2.30 td (7.0,

residual H2O

(7.8, 3.9)

1.70 br.t

4.6)

signal

1.26 m

3.50 br. dd (7.7, 3.9)

(12.7)

14

3.93 m

15

2.50 m

3-CH3

2.13 br. s

2.14 br. s

2.16 br. s

2.08 br. s

2.16 br. s

2.16 d (1.1)

5-CH3

1.76 br. s

1.78 br. s

1.77 br. s

1.71 br. s

1.76 d (0.7)

1.76 d (1.1)

7-CH3

0.91 d (6.6)

0.80 d (6.6)

0.80 d (6.6)

0.81 d (6.4)

11-CH3

0.98 d (7.0)

15-CH3

1.10 d (7.0)

13-

3.71 br. dd

3.71 dd (11.7,

3.49 br. d

3.72 dd (11.7,

CH2OH

(11.6, 3.9)

4.2)

(9.9)

4.2)

3.61 dd (11.6,

3.62 dd (11.7,

3.5)

3.5)

 

 

3.45 dd (9.9, 5.3)

526

ACS Paragon Plus Environment

 

3.62 dd (11.7, 3.3)

Page 29 of 30

527

Journal of Agricultural and Food Chemistry

Table 2. Position 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 3-CH3 5-CH3 7-CH3 11-CH3 15-CH3 13-CH2OH

1 168.6 119.2 152.4 129.9 140.4 48.5 34.6 136.8 127.1 28 33.6 74.3 58.7 170.8

2 168.7 119.3 152.4 128.3 142.1 40.7 28.9 28.9 27.6 24.8 33.5 74.8 58.7 170.9

19.5 18.7 20.4

19.5 18.5

56.6

56.7

3 168.3 118.8 152.8 129.7 141.1 48.8 30.8 36.6 26.6 25.1 35.9 76.3 36.5 66.3 41.6 174 19.5 18.6 19.7 13.3  

528 529

ACS Paragon Plus Environment

4 168.2 118.8 152.9 128.3 142.3 40.7 27.6 29.0 29.3 25.6 35.1 69.3 55.2 175.4

5 168.2 118.7 153.0 129.6 141.2 48.9 30.7 36.6 25.8 33.6 46.3 71.9 26.8 176.8

7 168.1 118.6 153.1 129.6 141.3 48.8 30.7 36.6 26.5 25.1 33.6 74.8 58.8 170.8

19.5 18.6

19.5 18.6 19.7 13.1

19.7 18.6 19.5

60.3

 

56.6

Journal of Agricultural and Food Chemistry

530

TOC

531 532

Table of Contents

HOH2C O

HOH2C

COOH O

O

COOH O

1

2 HOH2C O

Page 30 of 30

COOH O 7

533 534 535

ACS Paragon Plus Environment