product notes
MS. The HCT Ultra mass spectrometer has a high ion capacity and dynamic range, which make it suitable for quantitative proteomics and small-molecule applications. This instrument features smart precursor ion-selection strategies. PhosphoScan detects phosphorylated peptides on the fly in a single run. PeptideScan allows researchers to select multiply charged precursor ions for MS/MS without the need to perform charge state determination precursor scans. The HCT Ultra is compatible with the Proteineer and Metabolic Profiler. Bruker Daltonics
www.bruker-biosciences.com
HPLC chip. The Protein ID HPLC-Chip is a microfluidic device that reduces the sample size required for LC/MS analysis. The chip eliminates half of the fittings and connections that are typical of LC/MS systems, reducing leaks and dead volumes. A radio frequency tag stores experiment data, which are read every time the chip is inserted into the HPLC-Chip Cube on the mass spectrometer. Agilent
www.agilent.com
Informatics. The KnowItAll Informatics System Version 5.0 enables users to search data, such as structures, properties, and spectra, derived from multiple spectral techniques simultaneously across multiple databases. Visualization and data mining tools included in the software package simplify results. Special search tools allow researchers to weight the importance of each input and define how it factors into the final result. Bio-Rad
www.bio-rad.com
MS. The 3200 Q TRAP mass spectrometer, which is an update of the Q TRAP instrument, is a triple quadrupole–linear ion trap instrument for small-molecule identification and protein biomarker discovery. The new, smaller instrument has a twofold increase in sensitivity and a fivefold increase in S/N over the previous model. Applied Biosystems www.appliedbiosystems.com
LC. The Ettan MDLC is a flexible, multidimensional LC system that separates complex mixtures. The MDLC can be configured in several ways, depending on the researcher’s needs. The patented nanovalve results in low dead volumes and high 670
resolution. Preprogrammed methods are available. The instrument is made of biologically inert materials to ensure high yields of labile peptides. The flow-rate range is 200 nL/min–2 mL/min. The new system integrates with ESI-MS instruments for shotgun proteomics applications. GE Healthcare
www.gehealthcare.com
MALDI spotter. The versatile LCBOT 9000 functions as a MALDI spotter, a fraction collector, or an LC autosampler. As a spotter, the LCBOT 9000 places droplets from selected LC peaks onto a MALDI plate. The user can specify the start and end times of the peaks for droplet deposition. Between peaks, the device is in a standby mode, in which the effluent empties into a waste vessel. Alternatively, drops may be placed into vials for fraction collection. Brechbühler
www.brechbuehler.com
LC. The UltiMate 3000 nano and capillary LC system features electronically controlled active flow splitting, which improves gradient reproducibility and flow accuracy. The constant column flow of the system is independent of the column pressure and the type of solvent used. The system includes an integrated oven and valves and a microfraction collector. Dionex
www.dionex.com
SPR. The new T100 surface plasmon resonance instrument enables label-free protein interaction studies. It analyzes up to 384 samples during each run, over a broad kinetic range. The T100 has integrated buffer degassing for studies at high temperatures. It includes advanced software for assay development and analysis of several interaction parameters, including kinetics, affinity, specificity, concentration, and thermodynamics.
PCR. The Mx3005P Real-Time PCR system features 5 customer-selected filters. Researchers may select the filter path for fluorescence resonance energy transfer experiments. Software for primer and probe design is included. The PCR system handles 96-well plates, 8-strip tubes, and 200-µL tubes and is optimized for 25-µL reactions.
Bio products. The Life Science Chemistry Solutions brochure includes information on products for the biopharmaceutical, protein and peptide characterization, and proteomics fields. Chromatography columns, sample preparation kits, and a wide array of reagents are available.
Stratagene
Waters
www.stratagene.com
Workstation. The new Eppendorf epMotion 5070 liquid-handling workstation features automated pipetting and dispensing. Manual protocols can be easily adapted for use on the 5070. The workstation has an integrated control panel, which saves bench space. An optical sensor system ensures that the system reliably dispenses the appropriate amount of liquid. Buffers and other solutions may be dispensed into microfuge tubes or microwell plates. Brinkmann
www.brinkmann.com
Biacore
www.biacore.com
www.waters.com
MS. The AccuTOF Direct Analysis in Real Time (DART) instrument can analyze solids, liquids, and gases at atmospheric pressure and ground potential without sample preparation. Samples are placed between the DART ionization source and the mass spectrometer. The exact mass measurements obtained with the AccuTOF provide researchers with information that facilitates the identification of unknown compounds. Drugs, chemical warfare agents, and proteins are among the substances that have been precisely identified with the AccuTOF DART.
HPLC columns. ChromXP columns are designed for the high linear flow velocities used by the ExpressLC HPLC System, which features microfluidic pumping. Currently, the columns are packed with C18 material in 3- and 5-µm particle sizes, but other column types may be developed. Easy-to-use fittings allow the columns to be installed into the system without tools.
Robot. The BRT is a robotic transporter that moves plates in between devices, such as incubators and plate readers. The robot makes small movements, so lab space is conserved. The BRT extends the compatibility of the Biomek system with other devices.
Eksigent
Beckman Coulter
Journal of Proteome Research • Vol. 4, No. 3, 2005
www.eksigent.com
JEOL
www.jeol.com
www.beckman.com © 2005 American Chemical Society
