0 Copyright 1990 by the American Chemical Society
Volume 29, Number 3
January 23, 1990
Art ides
NMR Characteristics of Intracellular K in the Rat Salivary Gland: A 39K NMR Study Using Double-Quantum Filtering Yoshiteru Sea,*.* Masataka Murakami,t Eiji Suzuki,t Satoru Kuki,t Kuniaki Nagayama,g and Hiroshi Watarit Department of Molecular Physiology, National Institute for Physiological Sciences, Myodaiji, Okazaki 444, Japan, and Biometrology Laboratory, JEOL Ltd., Akishima, Tokyo 196, Japan Received January 19, 1989; Revised Manuscript Received September 7, 1989
ABSTRACT: Intracellular K of the perfused rat mandibular salivary gland was measured by 39K N M R spectroscopy at 8.45 T. Multiple-quantum N M R arising from multiple-exponential decay was used to eliminate the resonance due to extracellular K in the perfused gland a t 25 “C. The resonance due to intracellular K consisted of two Lorentzian signals stemming from the 11/ 2 >> aQ-'and r, >> w-l, the resonance splits into three resonances: a central peak corresponding to the 11/2>> 7 , >> w-I, the resonance is a superposition of a narrow resonance corresponding to the 11/2>
