Subscriber access provided by UNIV OF NEW ENGLAND ARMIDALE
Article
Novel RAS inhibitors poricoic acid ZG and poricoic acid ZH attenuate renal fibrosis via Wnt/#-catenin pathway and targeted phosphorylation smad3 signaling Ming Wang, Dan-Qian Chen, Lin Chen, Hui Zhao, Dan Liu, Zhi-Hao Zhang, Nosratola D. Vaziri, Yan Guo, Ying-Yong Zhao, and Gang Cao J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.8b00099 • Publication Date (Web): 31 Jan 2018 Downloaded from http://pubs.acs.org on February 1, 2018
Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.
Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
Page 1 of 44
Journal of Agricultural and Food Chemistry
1
Novel RAS inhibitors poricoic acid ZG and poricoic acid ZH attenuate renal fibrosis via Wnt/β-catenin pathway
2
and targeted phosphorylation smad3 signaling
3
Ming Wang†,@, Dan-Qian Chen†,@, Lin Chen†, Dan Liu†, Hui Zhao†, Zhi-Hao Zhang†, Nosratola D. Vaziri‡, Yan Guo†,§,
4
Ying-Yong Zhao*,†,‡, Gang Cao*,$
5 6
†
Sciences, Northwest University, No. 229 Taibai North Road, Xi’an, Shaanxi 710069, China
7 8
‡
§
13 14
Department of Internal Medicine, University of New Mexico, Comprehensive Cancer Center, Albuquerque, New Mexico, 87131, USA
11 12
Division of Nephrology and Hypertension, School of Medicine, University of California Irvine, Irvine, California 92897, USA
9 10
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Life
$
School of Pharmacy, Zhejiang Chinese Medical University, No. 548 Binwen Road, Hangzhou, Zhejiang 310053, China
@
Both the authors contributed equally to the research.
15 16
Corresponding authors:
17
Professor Ying-Yong Zhao, PhD, MD
18
School of Life Sciences, Northwest University, No. 229 Taibai North Road, Xi’an, Shaanxi 710069, China, Tel: +86 29
19
88305273; Fax: +86 29 88303572, E-mail:
[email protected];
[email protected] 20
Scientific Reports, American Journal of Nephrology, Editorial Board
21
Professor Gang Cao, PhD
22
School of Pharmacy, Zhejiang Chinese Medical University, No. 548 Binwen Road, Hangzhou, Zhejiang 310053, China;
23
Tel/Fax: +86 571 87195895; E-mail:
[email protected] 1 Environment ACS Paragon Plus
Journal of Agricultural and Food Chemistry
24
ABSTRACT: Renal fibrosis is a common endpoint of the progression of chronic kidney disease (CKD). Suppressing
25
the development and progression of renal fibrosis is essential in the treatment of kidney disease. Our previous study
26
demonstrated that the ethyl acetate extract of the surface layer of Poria cocos exhibited beneficial anti-tubulointerstitial
27
fibrosis. In this study, we isolated new diterpene (PZF) and triterpenes (PZG and PZH) and examined their anti-fibrotic
28
effect. TGF-β1 upregulated the collagen I protein expression in HK-2 cells and PZG and PZH treatment significantly
29
inhibited the upregulated collagen I expression (TGF group 0.59 ± 0.08 vs TGF+PZG group 0.36 ± 0.08, P
