Apr 6, 2015 - 2 adaptor binds to clathrin TD with a stoichiometry of 3:1. Assignment of .... Sepharose ion-exchange column with a 0 to 600 mM NaCl gradient.
Apr 18, 2018 - In recent years, there has been a resurgence of interest in melittin and its variants as their therapeutic potential has become increasingly ...
Publication Date (Web): April 18, 2018 ..... Another key feature of the pTM-melittin construct was the modification of the TrpLE sequence by replacing the two cysteines and six methionines with alanines and leucines, respectively,(36) to facilitate t
Apr 18, 2018 - (2) We performed SDSâPAGE on the recombinant melittin sample and the synthetic ... (41) The 3D 1Hâ15N NOESY-HSQC spectra of both 0 and 30% TFE samples at ...... U. S. A. 86, 6944â 6948, DOI: 10.1073/pnas.86.18.6944 ...... nano-li
Chemistry, California Institute of Tech- ... is the best qualitative discussion of these topics which the reviewer has seen. ... pages of text and appendixes, and more th in 3000 literature citations (some ... functions, and applications will follow.
Chemistry, California Institute of Tech- nology. ... measurable quantity of greatest interest to the chemist. ... Roberts' book is the best available ... 889 pages of text and appendixes, and more thm 3000 ... functions, and applications will follow.
Jan 3, 2013 - *Phone: (585) 275-3207. Fax: (585) 276-0205. .... Free Energy Landscape of GAGA and UUCG RNA Tetraloops. Sandro Bottaro , Pavel BanÃ¡Å¡ , JiÅÃ ... Atomistic Free Energy Model for Nucleic Acids: Simulations of Single-Stranded DNA and t
Starting backbone torsions measured with Pymol(46) are presented in Table S3 of ...... (30, 71) Here, UV melting studies provide additional evidence of the reduced .... in the gauche+ orientation, i.e., a torsion angle of â¼60Â° (Table 6 and Table S
Dec 28, 2016 - In this work, to examine the Ca2+ binding site of PRM-A, we performed a solid-state nuclear magnetic resonance experiment using 111Cd2+ as a ... (9) Because these three forms of complexes precipitate to make complicated aggregates, app
Jun 20, 2018 - *Department of Chemistry, Washington Univeristy in St. Louis, One Brookings Drive, St. Louis, MO 63130. E-mail: [email protected] Phone: ...
The Boiling Tube While standard-tapered glassware has largely replaced cork and rubber stopper fittings in the organic laboratory, the devices employed for smooth ebullition of solvents or solutions a t atmospheric pressure seem antiquated by eomparison. A random survey of sixteen lab manuals revealed that bailing chips, Teflon balls, wooden splints, and glass wool are commonly recommended. Each has its drawback, especially during a crystallization procedure in which a solution must be concentrated by boiling. Removal of any of the given materials before crystallization begins means certain loss of some of the solution. For years our students have employed a boiling tube which this writer first saw in use during his graduate work a t Brown University. The tube is made by fusing a 10- to 15-mm length of 6-mm Pyrex tubing onto a 6-mm Pyrex glass rod of any length. Insertion of the tube into a liquid to be boiled provides a steady flow of air bubbles, hence smooth boiling. The tube is as durable as any glass item when properly handled; it does not contaminate the solution, and it can be mass-produced cheaply by anyone with a n elemental knowledge of working glass. The most important advantage is its ease of removal from a solution from which crystallization is t o occur. A rinse with a small amount of the solvent ensures no material loss. An additional plus lies in the fact that the solid end of the tube may be used as a stirring rod. Tucker has described' a somewhat fragile "distillation bubble" which performs the desired function hut whose preparation is unnecessarily complicated and time-consuming. Knoebe12 suggested a U-shaped tube of 5-mm glass but this will not fit into small Erlenmeyer flasks. Also, if one forgets to remove this device before the solution cools mueh of the liquid will be drawn up into the tube. We could not find a commercially available boiling tube in any of thirteen catalogs that were cheeked." The tube described here is a scaled-up version of one suggested for use in miero-boiling point determinations.' Since the student of organic chemistry spends mueh of his or her time performing distillations and concentrating solutions, we suggest that it can be done more efficiently and with style by use of the boiling tube instead of those devices described in lab manuals. Tucker, S. H., J. CHEM. EDUC., 26,546 (1949). Knoebel, A. B., Chem. Analyst, 19,20 (1930) as abstracted in J. CHEM. EDUC., 7,683 (1930). However, one (Sargent-Welch, Catalog 119, p. 672) offers a boiling point capillary with two feet, appendages that are not needed on a micro tube. Shriner, R. L., Fuson, R. C., and Curtin, D. Y., "The Systematic Identification of Organic Compounds," 5th ed., John Wiley & Sons, Inc., New York, 1964, p. 37. Gettysburg College Gettysburg. Pennsylvania 17325