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Biological and Medical Applications of Materials and Interfaces
Optimizing the Fluorescent Properties of Nanoemulsions for Single Particle Tracking in Live Cells Xinyue WANG, Nicolas Anton, Pichandi Ashokkumar, Halina Anton, Tkhe Kyong Fam, Thierry F. Vandamme, Andrey S Klymchenko, and Mayeul Collot ACS Appl. Mater. Interfaces, Just Accepted Manuscript • DOI: 10.1021/acsami.8b22297 • Publication Date (Web): 07 Mar 2019 Downloaded from http://pubs.acs.org on March 11, 2019
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ACS Applied Materials & Interfaces
Optimizing the Fluorescent Properties of Nano-emulsions for Single Particle Tracking in Live Cells Xinyue Wang,a Nicolas Anton,a,* Pichandi Ashokkumar,b Halina Anton,b Tkhe Kyong Fam,b Thierry Vandamme,a Andrey S. Klymchenko,b,* Mayeul Collot b,* a
Université de Strasbourg, CNRS, CAMB UMR 7199, F-67000 Strasbourg, France
Laboratory of Biophotonic and Pathologies, CNRS UMR 7021, Université de Strasbourg, Faculté de Pharmacie, 74, Route du Rhin, 67401 Illkirch, France b
KEYWORDS. Lipid nanoparticles, nano-emulsions, dioxaborine, two-photon imaging, single particle tracking. ABSTRACT: Nano-emulsions (NEs) are biocompatible lipid nanoparticles composed of an oily core stabilized by a surfactant shell. It is acknowledged that the surface decoration with poly(ethylene glycol) (PEG), through the use of non-ionic surfactants, confers a high stealth in biological medium with reduced non-specific interactions. Tracking individual NEs by fluorescence microscopy techniques would lead to a better understanding of their behavior in cells and thus require the development of bright single particles with enhanced photostability. However, the understanding of the relationship between the physicochemical properties and chemical composition of the NEs, on the one hand, and its fluorescence properties of encapsulated dyes, on the other hand, remains limited. Herein, we synthesized three new Dioxaborine Barbituryl Styryl dyes (DBS) that displayed high molar extinction coefficients (up to 120,000 M-1.cm-1) with relatively low quantum yields in solvents and impressive fluorescence enhancement when dissolved in viscous oils (up to 0.98). The reported screening of 9 different oils allowed disclosing a range of efficient “oil/dye” couples, and understanding the main parameters that lead to the brightest NEs. We determine vitamin E acetate / DBS-C8 as the representative most efficient couple combining high dye loading capabilities and low aggregation induced quenching leading to
