Oxidative Potential by PM2.5 in the North China Plain: Generation of

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Ecotoxicology and Human Environmental Health 2.5

Oxidative Potential by PM in the North China Plain: Generation of Hydroxyl Radical Xiaoying Li, Xiaobi Michelle Kuang, Caiqing Yan, Shexia Ma, Suzanne E Paulson, Tong Zhu, Yuanhang Zhang, and Mei Zheng Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.8b05253 • Publication Date (Web): 30 Nov 2018 Downloaded from http://pubs.acs.org on December 1, 2018

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Oxidative Potential by PM2.5 in the North China Plain: Generation of Hydroxyl

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Radical

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Xiaoying Li1#, Xiaobi M. Kuang2#, Caiqing Yan1, Shexia Ma3, Suzanne E. Paulson2,

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Tong Zhu1, Yuanhang Zhang1, and Mei Zheng1*

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1SKL-ESPC

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Peking University, Beijing 100871, China

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2Department

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Angeles, Los Angeles, CA 90095, USA

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3South

and BIC-ESAT, College of Environmental Sciences and Engineering,

of Atmospheric and Oceanic Sciences, University of California at Los

China Institute of Environmental Sciences, Ministry of Environmental

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Protection, Guangzhou 510655, China

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* Corresponding author: Mei Zheng, [email protected]

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# Both

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ABSTRACT

contributed equally as the first author.

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Adverse health effects of ambient PM2.5 (dp99%)

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were obtained from J&K Scientific Ltd., China. Sodium chloride (guaranteed reagent

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grade, GR) was from Xilong Chemical Co., Ltd., China. Disodium Terephthalate (TA,

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ACS) was from Tokyo Chemical Industry Co., Ltd., Japan. Sodium citrate tribasic

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dihydrate (99.0%), ascorbic acid sodium salt (Asc, >99%), uric acid sodium salt (UA,

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ACS), nitric acid (Cit, >69%), 2,2,2-Trifluoroethanol, and chelex-100 (95%) were

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purchased from Sigma Aldrich (St. Louis, MO, USA).

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Sampling and chemical analysis. Ambient PM2.5 samples were collected at an

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urban (Beijing) and a suburban (Wangdu) site in NCP from 9th June to 8th July, 2014

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(Figure S1, Supporting Information) during the CAREBEIJING-NCP project. Wangdu

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is a small town in Hebei Province, and close to Baoding (35 km) and Shijiazhuang (90

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km), which are two major industrial cities in NCP. Consecutive 23-h PM2.5 samples

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were collected in Beijing, typically starting from 9:00 a.m. and ending at 8:00 a.m. the

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next day. PM2.5 samples in Wangdu were collected during the daytime (from 8:00 a.m.

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to 5:30 p.m.) and nighttime (from 6:00 p.m. to 7:30 a.m. the next day). Teflon filters

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were weighted before sampling and re-weighted after sampling using a microbalance

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(Mettler Toledo) with the sensitivity of 0.00004 g to determine the mass concentration

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of PM2.5. Chemical compositions of PM2.5 were analyzed, including OC, EC, water-

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soluble ions (Na+, NH4+, K+, Mg2+, Ca2+, NO3-, SO42-) by ion chromatography (ICS-

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2000 and ICS-2500, DIONEX), and trace metals (Al, Fe, Mn, Ti, Co, Cr, Ni, Cu, Pb,

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Zn, Cd, V, As, Se, Mo, and Co) by inductively coupled plasma-mass spectrometry

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(ICP-MS). A detailed description of sampling and chemical analyses is provided in

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Text S1.

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Surrogate Lung Fluid (SLF) solution. All extractions were performed in a cell-free

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SLF solution, which is a surrogate of lung fluid, but it has to be recognized that it is

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different from actual lung fluid. It consists of phosphate-buffered saline (PBS,

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including 114 mM NaCl, 7.8 mM Na2HPO4 and 2.2 mM KH2PO4 to buffer the solution 5 / 29

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at pH 7.2-7.4), four antioxidants (200 µM Asc, 300 µM Cit, 100 µM GSH, and 100 µM

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UA), and TA (10 mM).30,41-48 The PBS was treated by column chromatography with

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Chelex-100 resin to remove metals, stored in the refrigerator, and generally used within

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one month. Four antioxidants were freshly prepared and added to the PBS just prior to

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extraction.

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Particle extraction and quantification of ·OH. In this study, half of a Teflon filter

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was first treated with 50 µL of 2,2,2-Trifluoroethanol to increase water-solubility of

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particles on the filters.42,47 It is also added to filed blank filter extractions and the

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resulting signals are subtracted off to correct the sample results. Vidrio et al.47 reported

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that TFE could enhance ·OH production by 80±40% compared to the same sample

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extracted by SLF in the absence of TFE. The average absolute mass of PM2.5 on

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extracted filters is 238±143 μg (mean±standard deviation) and 410±228 μg in Beijing

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and Wangdu, respectively. Before each filter was placed in a Teflon dish, 10 mL of

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SLF solution was added. TA was added and reacted with ·OH to produce TAOH, which

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is stable and strongly fluorescent. TAOH was detected at excitation/emission

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wavelength (λex/λem) of 320/420 nm using a sensitive fluorometer (Cary Eclipse,

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Varian), and then aliquots of 200 µL of solution were measured at 0, 20, 40, 60, 80, 100

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and 120 minutes to determine the amount of TAOH formed. For each sample and blank

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filter, TAOH was measured three times and the mean value of the results was taken as

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the final concentration of TAOH. The production of ·OH by PM2.5 in SLF increased

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linearly during two hours of extraction, with the formation rate of 0.49 ng/μg·PM2.5·h

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and 0.52 ng/μg·PM2.5·h in Beijing and Wangdu, respectively (Figure S2). To better

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compare with other studies which applied similar method with this study30,33,42,43,47, the

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production of ·OH showed in this study was presented as the total ·OH generated in

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SLF after 2-hour reaction, normalized to PM2.5 mass (ng/µg·PM2.5) and air volume

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(ng/m3·air), rather than the formation rate of ·OH.

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Calibration with TAOH standards at 50, 100, 200, 500 and 800 nM was performed

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daily. 10 mM of TA in the SLF solution is sufficient to react with >98% of ·OH. The

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concentration of ·OH in each solution was determined using Equation 1,

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[·OH] = [TAOH] / yTAOH 6 / 29

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Equation 1

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where [TAOH] is the measured concentration of TAOH, yTAOH is the molar yield of

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TAOH produced from the reaction of ·OH with TA in SLF, which is 0.35 at pH 7.2.42,49

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The SLF solution blanks and field blanks were treated in the same way with PM2.5

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samples. The amount of TAOH formed in the SLF solution blanks was less than 1% of

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the amount of TAOH formed in samples, and thus could be ignored. The results of the

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field blanks was ~10% of the amount of TAOH formed in samples, and were subtracted

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off to correct the sample results.

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RESULTS AND DISCUSSION

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High ·OH production (ng/m3·air) by PM2.5 in heavily polluted days. Figure 1

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shows the mass concentration of PM2.5 and ·OH production (ng/m3·air) by PM2.5 in

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Beijing and Wangdu. The daily variation of PM2.5 in Beijing and Wangdu is similar,

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indicating the regional nature of air pollution in NCP. However, the mass

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concentrations of PM2.5 in Wangdu in most samples are higher than those in Beijing,

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although it is a suburban site in NCP. The average mass concentrations of PM2.5 in

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Beijing and Wangdu are 51.4±31.5 µg/m3 and 70.5±37.4 µg/m3, respectively (Table 1).

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PM2.5 mass concentrations in the nighttime in most samples are slightly higher

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compared to daytime samples in Wangdu. The ·OH production (ng/m3·air) shows the

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similar day-to-day variation trend with PM2.5, and it is higher in Wangdu compared to

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Beijing. The average ·OH production is 47.0±16.5 ng/m3·air and 61.0±21.9 ng/m3·air

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in Beijing and Wangdu, respectively. The air in Wangdu exhibits higher oxidative

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potential due to its larger concentration of PM2.5.

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Considering the National Ambient Air Quality Standard of China and the PM2.5

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concentration range during our sampling period, samples collected on days with daily

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average PM2.5 concentration below 35 µg/m3 are defined as clean days, 35 µg/m3 to 75

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µg/m3 as lightly polluted days, and above 75 µg/m3 as heavily polluted days. From

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clean days to heavily polluted days, ·OH production increases 2.0 and 1.6 times in

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Beijing and Wangdu, respectively. As Figure 2 and Table 1 show, the ·OH production

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(ng/m3·air) increases with PM2.5 concentration in both Beijing and Wangdu, and the

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increasing trend goes to flat when PM2.5 concentration reaches higher level. It is 7 / 29

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consistent with the integrated exposure-response in epidemiologic studies, which

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describes the relationship between health endpoints (e.g., ischemic heart disease, stroke,

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chronic obstructive pulmonary disease) and exposure to PM2.5.50,51 It indicates that as

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PM2.5 concentration increases, e.g., for every increased 10 µg/m3 of PM2.5, less ROS

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are produced.

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The ·OH production per unit mass (ng/µg·PM2.5) decreases with PM2.5

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concentration. Figure 3 presents the production of ·OH per unit mass of PM2.5

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decreases with increasing ambient PM2.5 concentration. Regardless of the sampling

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location, a power function is observed between ·OH production (ng/µg·PM2.5) and

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ambient PM2.5 mass concentration, and similar trend was also observed in Seoul,

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Korea32. There are two possible reasons for the observed pattern. One possible reason

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is that the increased mass in PM2.5 has little influence on ·OH generation, and the other

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is that the possible inhibiting effect of high levels of redox active metals in the

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extraction solution on ·OH generation, as the reaction to produce ·OH is a reversible

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reaction.

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For the first possibility, SO42-, NO3- and NH4+ dominate the increment of PM2.5 in

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both Beijing (80%) and Wangdu (83%) (Table S1), while they do not contribute to the

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formation of ROS due to their non-redox activity45,46. Many studies reported that it is

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the redox-active components in PM2.5 inducing the formation of ROS, including trace

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metals, quinones, and HULIS28,30,34,35, whereas SO42-, NO3- and NH4+ are non-redox

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active.

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The second possibility is the inhibiting effect of redox active metals to ·OH formation

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in SLF, due to their high concentrations in heavily polluted days. Charrier and

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Anastasio30 investigated the concentration dependence of ·OH production from water-

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soluble Fe, Cu, and quinones at atmospherically relevant concentrations and found that

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·OH produced by water-soluble Cu in simplified laboratory solutions is linearly related

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to the Cu concentration at low concentrations, but flattens out above ~200 nM. In this

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study, the average concentration of Cu (digested with strong acids) in the extraction is

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110 ± 82 nM and 204 ± 175 nM in Beijing and Wangdu, respectively. While the 8 / 29

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concentrations of water-soluble Cu in the extraction solutions were not measured, Cu

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is one of the most soluble metals in particles, with reported fraction soluble compared

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to total Cu in the 60~80% range52,53. Similar concentration-dependent behavior was

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observed for several quinones, but not for ·OH produced by water-soluble Fe.30

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However, Charrier and Anastasio30 also confirmed that the mixtures of Fe and Cu

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mitigate the behavior and result in a synergistic increase in ·OH production. Because

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the concentrations of Cu in the extraction solutions are not too high in this study, a

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similar concentration-dependence may not be occurring. However, future study is

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needed to further investigate the inhibiting effects of redox active metals (e.g. Fe, Mn,

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Cu) and/or quinones in PM2.5.

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Therefore, the decrease of ·OH production (ng/µg·PM2.5) with PM2.5 concentration

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in this study is most likely due to the fact that the increased PM2.5 in NCP is mainly

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secondary inorganic components (sulfate, nitrate, and ammonium) and these

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components are not the key species that contribute to the generation of ROS.

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Correlations between chemical species in PM2.5 and ·OH production. To

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investigate which species are more responsible for ·OH generation in Beijing and

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Wangdu, chemical species in PM2.5 were analyzed and Pearson correlation analysis was

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conducted between ·OH production and chemical species in PM2.5.

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Correlations between ·OH production and major chemical components in PM2.5. The

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average mass concentration of major chemical species in PM2.5 in Beijing and Wangdu

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is shown in Figure 4. The average concentrations of OC and EC in Wangdu are slightly

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higher than Beijing (1.44 times for OC and 1.25 times for EC), while the relative

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contributions of OC and EC to PM2.5 are similar (Figure 5). Similar to OC and EC, the

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concentrations of SO42- and NH4+ in Wangdu are 1.50 and 1.30 times higher compared

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to those in Beijing, and this may be due to more coal combustion (sulfur dioxide, SO2)11

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and livestocks and agricultural emissions (ammonia gas, NH3)54 in the suburban site

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Wangdu. The concentrations of NO3- are 10.7±10.2 μg/m3 and 10.6±10.0 μg/m3 in

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Beijing and Wangdu, respectively, but the relative contributions of NO3- to PM2.5 in

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Beijing is higher than that in Wangdu, due to higher contributions from vehicle exhaust. 9 / 29

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Figure 6 presents the Pearson correlation between ·OH production and PM2.5

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chemical components. It can be seen that OC, not water-soluble organic carbon

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(WSOC), is strongly correlated with ·OH production in Beijing and these relationships

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are not statistically significant in Wangdu. WSOC, HULIS and quinones, have all been

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associated with ROS formation as shown in other studies.30,31,34,55-57 Some studies have

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demonstrated that the aging process of organic aerosol could increase the oxidative

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potential of PM2.5.52,58,59 Liu et al.14 also reported that WSOC has a high contribution

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to ROS activity in Beijing and it appears to be associated with heavy traffic emissions.

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In addition, Lin et al.60 found that the oxygenated products of PAHs in Beijing are

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quinones, and secondary formation is a significant source of PM2.5 toxicity in non-

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heating season due to the generation of harmful ROS by quinones.

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Similar to OC, EC is also strongly correlated with ·OH production in Beijing, but not

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in Wangdu. This might be related to the different sources of PM2.5 in Beijing and

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Wangdu, which result in different atmospheric composition of PM2.5, especially for

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carbonaceous aerosol. In Beijing, vehicle exhaust is one of the most important sources

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of OC and EC in PM2.5 in summer, accounting for 63% of the carbonaceous components

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in PM2.5.61,62 Wangdu is a suburban site, with industry from its surrounding area and

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biomass burning exhibiting higher importance and impact.63 Several studies have found

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associations between EC and adverse health effects, but the mechanism is not well

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understood.64-67 Hu et al.58 found the high correlation between EC and ·OH, and

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proposed this correlation is actually due to the correlation between OC and ·OH, since

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EC and OC are normally from the same sources, such as vehicle exhaust. It has also

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been found that fresh emitted BC has low oxidative potential, while it increases as soot

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(or EC, BC) particles oxygenated by O366 or secondary organic carbon, PAHs and

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quinones adsorbed on soot surfaces25-27,52,59. Therefore, the good correlation between

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EC and ·OH production in Beijing suggests that the measured EC in ambient samples

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is more aged EC.

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However, the correlations between ·OH production and SO42-, NO3-, and NH4+ are

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all negative, and such negative correlations are stronger for Beijing relative to Wangdu

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(Figure 6). This is consistent with the findings of Hu et al.68 and Ntziachristos et al.69, 10 / 29

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indicating SO42-, NO3-, and NH4+ do not have a direct pathway to induce the formation

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of ROS. This negative correlation provides supportive evidence to explain the findings

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that the production of ·OH per unit mass (ng/μg·PM2.5) decreases with ambient PM2.5

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concentration, because the majority of increased PM2.5 mass is mainly composed of

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SO42-, NO3-, and NH4+.

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Correlations between ·OH production and trace metals in PM2.5. It should be noticed

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that the trace metals analyzed in this study are digested with strong acids and they are

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not water-soluble fraction only. The difference of metals in PM2.5 at the two sites

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(Beijing and Wangdu) is significant (Figures 4, 5). In Beijing, the average mass

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concentration of all metals is 0.54±0.17 µg/m3, accounting for 1.1% of PM2.5, while

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their contribution is 2.4% in Wangdu (1.67±1.09 µg/m3). Several studies have shown

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that water-soluble metals which undergo redox cycle are strongly associated with ROS

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formation, of which, Fe, Cu, and Mn are more active.14,30,31,34,35,43,46 The relative

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contributions of Fe, Cu, and Mn (digested with strong acids) to all metals in Beijing are

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almost double those in Wangdu, especially Fe, which account for 42% of all metals in

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Beijing vs. 24% in Wangdu (Figure 5). The relative contributions of Cu and Mn to all

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metals in all samples are quite low, but it is still higher in Beijing than Wangdu. Redox

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inactive Al accounts for nearly half of all metals in Wangdu samples (45%), about two

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times higher than that in Beijing (25%). The relative contributions of other metals are

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much the same at both sites. Therefore, trace metals considered to be more redox active

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are more enriched in the urban site Beijing than the suburban site Wangdu.

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Quite a few studies have demonstrated that different chemical species in PM2.5 have

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different abilities to contribute to the generation of ·OH in SLF.14,28-30,34,35 For example,

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in simplified solutions, water-soluble Cu and Fe generate the most ·OH, with additional

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contributions from a subset of quinones.30 A recent published paper showed that water-

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soluble transition Cr and Zn are correlated to oxidative potential of PM2.5, and As and

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V have a high contribution to ROS activity in Seoul, Korea.32 A lab study also found

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that both nano- TiO2 and ZnO could induce the formation of ·OH.70 Therefore, the

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difference in trace metals in PM2.5 could also be one of the most important reasons for

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the difference for the oxidative potential of PM2.5 in Beijing and Wangdu, besides the 11 / 29

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aged OC and EC.

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As Figure 6 shows, the metals measured with strong acid digestion are strongly

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correlated with ·OH production both in Beijing and Wangdu, although the extent of the

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correlation varies with species. Fe, Ba, Mn, Al, Ca2+, Mo, and Se all have strong

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correlations (p