Page 1 J. Am. Chem. SOC. 1989, Ill, 8921-8923 8921 Scheme 11

8921

J . A m . Chem. SOC.1989, I l l , 8921-8923 Scheme 11. Reactions Tested by Using the 3-PGA Method To Regenerate A T P According to Scheme I"

c Mp

-

E.C. 2.7.4.3

E.C. 2.7.1.40

AT PI AD^

Or

UMP

PEP

Or

UDP

Guanylate klnase

E.C. 2.7.1.40

-3-

ATPIADP

w

UTP 17 mmol

(2)

GTP

(3)

18 mmol

PEP

ODP

Nucleoside monophosphate klnase UMP

Or

Pyruvate kinase

E.C. 2.7.4.8

OMP

(1)

CTP Pyruvate kinase 202 "01

Adenylate klnase cDp

Pyruvate kinase

E.C. 2.7.4.4

~w

E.C. 2.7.1.40

UDP

(4)

UTP

PEP

14 mmol

ATPIADPJ

The primary advantage of the PGA method as a route to PEP is that it requires less time, produces less organic waste, and is more convenient than the chemical synthesis. Its starting materials are commercially available. It is, however, more expensive. The convenience outweighs the difference in cost for reactions carried out on a scale