Bioconjugate Chem. 2007, 18, 1360
1360
CORRECTIONS Volume 18, Number 3, May/June 2007.
Kenneth J. Oh, Kevin J. Cash, Verena Hugenberg, and Kevin W. Plaxco* PEPTIDE BEACONS: A NEW DESIGN FOR POLYPEPTIDE-BASED OPTICAL BIOSENSORS We regret the omission of two prior publications describing similar peptide beacon architectures for the detection of biomolecules that have recently come to our attention. Wei and co-workers (1) demonstrated a peptide-based, fluorescent antibody detection concept that invokes a binding-induced conformational change to detect human chorionic gonadotrophin (hCG) antibody. Similarly, Geoghegan and co-workers (2) invoked two fluorophores at the terminal ends of a phosphopeptide recognized by an SH2 protein domain, resulting in increased fluorescence when titrated against the target. While neither technique invokes the binding-induced change in dynamics exploited in our peptide beacon architecture, both are visible-light, “signal on” detection systems, whereby the presence of target is indicated by the increase in fluorescence intensity.
LITERATURE CITED (1) Wei, A. P., Blumenthal, D. K., and Herron, J. N. (1994) Antibody-mediated fluorescence enhancement based on shifting the intramolecular dimer T monomer equilibrum of fluorescent dyes. Anal. Chem. 66, 1500-1506. (2) Geoghegan, K. F., Rosner, P. J., and Hoth, L. R. (2000) Dyepair reporter systems for protein-peptide molecular interactions. Bioconjugate Chem. 11, 71-77. BC7001852 10.1021/bc7001852 Published on Web 06/21/2007
