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Perfluoroalkylated substances effects in Xenopus laevis A6 kidney epithelial cells determined by ATR-FTIR spectroscopy and chemometric analysis Eva Gorrochategui, Silvia Lacorte, Romà Tauler, and Francis Luke Martin Chem. Res. Toxicol., Just Accepted Manuscript • DOI: 10.1021/acs.chemrestox.6b00076 • Publication Date (Web): 14 Apr 2016 Downloaded from http://pubs.acs.org on April 17, 2016
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Chemical Research in Toxicology
Perfluoroalkylated substances effects in Xenopus laevis A6 kidney epithelial cells determined by ATR-FTIR spectroscopy and chemometric analysis Eva Gorrochategui1, Sílvia Lacorte1, Romà Tauler1 and Francis L. Martin2,3* 1
Department of Environmental Chemistry, Institute of Environmental Assessment and
Water Research (IDAEA), Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, 08034, Catalonia, Spain; 2Centre for Biophotonics, Lancaster Environment Centre, Lancaster University, Lancaster LA1 4YQ, UK; 3School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, UK
*Corresponding author: Francis L. Martin PhD; E-mail:
[email protected]; Tel.: +44 (0)1524 510206
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Chemical Research in Toxicology
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Chemical Research in Toxicology
Abstract: The effects of four perfluoroalkylated substances (PFASs), namely,
perfluorobutanesulfonate
(PFBS),
perfluorooctanoic
acid
(PFOA),
perfluorooctanesulfonate (PFOS) and perfluorononanoic acid (PFNA) were assessed in Xenopus laevis A6 kidney epithelial cells by attenuated total reflection Fouriertransform infrared (ATR-FTIR) spectroscopy and chemometric analysis. Principal component analysis-linear discriminant analysis (PCA-LDA) was used to visualize wavenumber-related alterations and ANOVA-simultaneous component analysis (ASCA) allowed data processing considering the underlying experimental design. Both analyses evidenced a higher impact of low-dose PFAS-treatments (10-9 M) on A6 cells forming monolayers, while there was a larger influence of high-dose PFAS-treatments (10-5 M) on A6 cells differentiated into dome structures. The observed dose-response PFAS-induced effects were to some extent related to their cytotoxicity: the EC50-values of most influent PFAS-treatments increased (PFOS
