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Energy & Fuels
Petroleum biodegradation effects on polar acidic compounds and correlation with their corresponded hydrocarbon fractions Célio F. F. Angolini †, Ramsés Capilla ‡, Anita J. Marsaioli *† †
Institute of Chemistry, University of Campinas – UNICAMP, Campinas, São Paulo CP 6154,
CEP 13083-97, Brazil ‡
PETROBRAS/CENPES/PDGEO/GEOQ, Av. Horácio Macedo, 950. Cidade Universitária, Ilha
do Fundão, Rio de Janeiro, CEP - 21941-915, Brazil Petroleum biodegradation, Polar compounds and Petroleum biodegradation scale.
ABSTRACT
A set of five oil samples from the same source rock, classified at different levels of biodegradation, was evaluated by high-resolution mass spectrometry (Orbitrap). O-containing acidic compounds from the acidic oil fractions were characterized by determining their molecular formulae. The effects of biodegradation were detected in all O classes and were consistent with the biodegradation of neutral compounds. Additionally, we found a slightly biodegraded sample that contained acidic compounds, biomarkers of highly biodegraded sample.
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Energy & Fuels
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These results led us to propose a scale of biodegradation using acidic compounds, particularly those containing two oxygen atoms (carboxylic acids).
1. INTRODUCTION Microorganisms may degrade petroleum in a low entropy process via different biological pathways1, 2 at rates that depend on the availability of inorganic and organic electron acceptors.3, 4
Biodegradation commonly occurs in low-temperature reservoirs (
