REPORT
(1) Critical evaluation of all of our methods—which we wish to encourage (2) Proliferation of inadequately described methods—which we wish to curtail (3) Sound appraisal of AAS methods through interlaboratory testing programs— which we wish to facilitate (4) Agreement on recommended practices—which we wish to expand.
Especially designed for Gel Filtration Chromatography Ion Exchange Chromatography
Sephadex
Laboratory Columns A product of over six years' research know-how brings you these "exclusive' column features:
I consider it essential that we achieve each of these objectives. Acknowledgments
The assistance of many individ uals in industrial, academic, and government laboratories made this report possible, and their interest and encouragement is gratefully acknowledged. The author is par ticularly indebted to M. D. Cooper for many stimulating discussions, and to members of Committees E-2 and E-3 of ASTM for the informa tion that they provided.
1 AQUEOUS AND ORGANIC SOLVENT SYS TEM COLUMNS —only columns specially de signed for use in these chromatographic systems 2 MIXING CHAMBER-of less than 1/10% of bed volume minimizes sample dilution to insure optimal zone sharpness for critical separations 3 INERT NYLON OR TEFLON NETTING-on the sample applicator, bottom endpiece or flow adaptor eliminates adsorption of biologic material 4 DESCENDING TO RECYCLING OR UPWARD FLOW—easily converted by replacing both endpieces with new Sephadex Flow Adaptors
Literature C i t e d (1) J. P . Riley and D . Taylor, Anal. Chim. Acta, 40, 479 (1968). (2) G. H . Morrison and R. K. Skogerboe, in "Trace Analysis: Physical Meth ods," G. H . Morrison, Ed., Interscience, New York, 1965, pp. 10-13. (3) S. 11. Koirtvohann, At. Aba. News letter, 6, 77 (1967).
STANDARD LABORATORY COLUMN FOR DESCENDING CHROMATOGRAPHY
RECYCLING UPWARD FLOW CHROMATOGRAPHY WITH NEW FLOW ADAPTORS
(4) G. E . F . Lundell, I N D . E N G . C H E M . ANAL. ED., 5, 221 (1933).
(5) Ε . Β . Sandell, in "Treatise on Ana lytical Chemistry," I . M. Kolthoff and P. J. Elving, Eds., P a r t I, Vol. 1, Inter science Encyclopedia, Inc., New York, 1959, p. 38. (6) B. Meddings and H. Kaiser, At. Abs. Newsletter, 6, 28 (1967). (7) It. E . Thiers, in "Methods of Bio chemical Analysis," D . Glick, Ed., I n terscience, New York, Vol. V, 1957, pp. 273-335. (8) "Reagent Chemicals," American Chemical Society, Washington, D . C , 1961. (9) D . It. Weir and R. P . Kofluk, At. Abs. Newsletter, 6, 24 (1967). (10) K. M. Cellier and H . C. T. Stace, Appl. Spectry., 20, 26 (1966). (11) M. L. Parsons and J. D . Wincfordner, Appl. Spectry., 21, 368 (1967).
5 SAMPLE APPLICATOR-distributes the sam ple evenly over the bed surface to insure sharp zones for critical separations and protects as well as stabilizes the bed 6 SPECIAL DESIGN BED SUPPORT-eliminates troublesome sintered glass disc
AVAILABLE SEPHADEX COLUMNS AND ACCESSORIES SEPHADEX COLUMNS AQUEOUS SYSTEMS
Type Κ 9.Ί 5 Κ 9/30 Κ 9/60 Κ 15/30 Κ 15/90 Κ 25 45 Κ 25/45 Κ 25/100 Κ 25/100 "Jacketed" Κ 50/100 "Jackeled" Κ 100/100 "Jacketed"
0.9x15 0.9x30 0.9x60 1.5x30 1 5x90 25x45 2 5x45 2.5x100 2.5x100 5.0x100 10.0x100
Cooling Jacket
ACCESSORIES Sample Applicator
S S S
S —
Ο S S
—
S
SEPHADEX COLUMNS SR" RESISTANT TO ORGANIC SOLVENTS 2.5x45 2.5x100
SR25'45 SR25/100
—
S - Standard Accessories
s
O-- Optional Accessories
FLOW ADAPTORS·
The above is an abridgment of a paper de livered at the Annual Meeting of the American Society for Testing and Ma terials held in San Francisco, June 25, 1968, at the Symposium on Atomic Ab sorption Spectroscopy. The full paper will appear with other papers presented at this meeting in a special ASTM technical pub lication, "Atomic Absorption Spectros copy," ASTM STP 443.
Flow Adaptors To fit all Κ 25 Sephadex Lab. Cok,mnS "Two Flow Adaptors should be used when conducting upward flow or recycling chromatography
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