Photoinduced electron transfer in self-associated complexes of

J. S. Zhou, E.S. V. Granada, N. B. Leontis, and M. A. J. Rodgers*. Contribution from ...... express our thanks particularly to Dr. W. E. Ford for his ...
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J . Am. Chem. SOC.1990, 112, 5074-5080

did not obtain work of formation results for cavities substantially larger than the solvent molecules, and that constitutes the chief limitation on the conclusions that are drawn here. Calculations of cavity works for much larger cavities will require alternative methods.

Acknowledgment. We thank Michael A. Wilson for help in acquisition of some of the data. This work was supported in part by NASA-Ames-UC Berkeley Intergovernmental Personnel Exchange Agreement NCA-2 315 and by the Numerical Aerodynamics Simulation (NAS) program.

Photoinduced Electron Transfer in Self-Associated Complexes of Several Uroporphyrins and Cytochrome c J. S . Zhou, E. S. V. Granada, N. B. Leontis, and M. A. J. Rodgers* Contributionfrom the Center for Photochemical Sciences, Bowling Green State University, Bowling Green, Ohio 43403. Received November 13, I989

Abstract: Photoinduced electron transfer between cytochrome c and free base and metallouroporphyrin (Up, MUp) has been studied. Difference absorption spectrophotometry showed that the electrostatic interactions between Up and cytc(II1) result in their forming a self-associated 1:l complex in the ground state with a binding constant that depends upon the ionic strength. In the complex, the photoexcited uroporphyrin singlet state was quenched through a static interaction with the protein. Even under the most favorable quenching conditions, Le., when all porphyrin was complexed, residual fluorescence was noted. More significantly the excited singlet state of the complex was shown to undergo small, but significant, intersystem crossing. These triplet states rapidly underwent an electron-transfer process that yielded transiently the Fe(I1) form of the protein. This is the first observation of such a process from a porphyrin/cytochrome self-association complex. Both the rates of bimolecular electron transfer between uncomplexed partners and intramolecular electron transfer from the uroporphyrin triplet to cytochrome c, as well as the thermal intramolecular back-reaction, have been measured by transient kinetic spectroscopy. The rate constants of intramolecular electron transfer for zinc uroporphyrin/cytochrome c and zinc cytochrome c/ferriuroporphyrin have been also determined. These three couples allow us to estimate approximately the reorganization energy X in the semiclassical electron-transfer theory.

It is now well-known that biological energy is channeled through the photosynthetic and mitochondrial respiratory chain via electron-transfer reactions.'-s This has stimulated a variety of studies, especially on fixed-site electron transfer in proteins, in an attempt to understand long-range electron-transfer reactions in biological systems. I n recent years, mitochondrial cytochrome c has been one of the proteins most widely used as the study system,6 since it is well characterized in terms of both primary and tertiary structure and since X-ray crystallographic structures are available for a number of native cytochrome c systems, thereby facilitating model building.' The approaches applied by a number of research groups to fix electron-transfer reaction centers at given distances in cytochrome c systems are either to covalently bond a donor and/or acceptor residue to specified sites on the cytochrome c surface8-l4 or to rely on electrostatic self-association between (1) Chance, B., DeVault, D. C., Frauenfelder, H., Marcus, R. A,, Schrieffer, J. R., Sutin, N., Eds. Tunneling in Biological Systems; Academic Press: New York, 1979. (2) Hatefi, Y. Annu. Rev. Biochem. 1985, 54, 1015. (3) Dixit, B. P. S. N.; Vanderkooi, J. M. Curr. Top. Bioenerg. 1984, 13, 159. (4) Michel-Beyerle, M. E., Ed. Antennas and Reaction Centers of Photo-Synthetic; Eacterio; Springer-Verlag: Berlin, 1985. ( 5 ) Govindjee, Ed. Photosynthesis. Energy Conversion by Plants and Bacteria; Academic Press: New York, 1982; Vol. 1. (6) Pielak, G . J.; Concar, D. W.; Moor, G. R.; Williams, R. J. P. Protein Eng. 1987, I . 83-88. (7) Takano, T.; Dickerson, R. E. J. Mol. Biol. 1981, 153,79-94. Takano, T.;Dickerson. R. E. J. Mol. Biol. 1981. 153. 95-115. &hi. H.: Hata. Y.; Tanaka. N.: Kakudo. M.: Sakurai. T.: Aihara. S.: Morita. Y. J. Mol. Biol. 1983, 166. 407-418. (8) Crutchley, R. J.; Ellis, W. R.; Gray, H. B. J . Am. Chem. SOC.1985, 107. 5092-5094. (9) Mayo, S . L.; Ellis, W. R.; Crutchley, R. J.; Gray, H. B. Science 1986, 233, 948-952.

0002-7863/90/ 151 2-5074$02.50/0

cytochrome c and its ~ a r t n e r . ' ~ - ~ ~ On the basis of these concepts, we decided to study cytochrome c, which has six cationic lysine residues at its surface near the solvent-exposed heme site, and as redox partner use uroporphyrin, which has eight carboxylate residues on short side chains disposed around the periphery. Our hypothesis was that this pair would form an electrostatic self-associated complex in aqueous solution that could perhaps be induced to undergo electron transfer when the porphyrin was excited into its triplet state. This resembles the system employed by Cho et who used cytochrome c (10) Bechtold, R.; Gardineer, M. B.; Kazmi, A,; Van Hemelryck, B.; Isied, S . S . J . Phys. Chem. 1986, 90. 3800-3804. (1 I ) Bechtold, R.; Kuehn, C.; Lepre, C.; Isied, S. S. Nature 1986, 322, 286-288. (12) Elias, H.; Chou, M. H.; Winkler, J. R. J. Am. Chem. Soc. 110,1988, 429-434. (13) Conrad, D. W.; Scott, R. A. J . Am. Chem. Soc. 1989, I l l , 346 1-3463. (14) Meade, T. J.; Gray, H. B.; Winkler, J. R. J . Am. Chem. SOC.1989, 111. 4353-4356. ( 1 5 ) Ho,P. S.; Sutoris, C.; Liang, N.; Maragoliash, E.; Hoffman, B. M. J . Am. Chem. SOC.1985, 107, 1070-1071. (16) Cheung, E.; Taylor, K.; Kornblatt, J. A.; English, A. M.; McLendon, G.; Miller, J. R. Proc. Natl. Acad. Sri. U.S.A. 1986, 83, 1330-1333. (17) Liang, N.; Kang, C. H.; Ho, P. S.; Maragoliash, E.; Hoffman, B. M. J. Am. Chem. SOC.1986, 108,4665-4566. (18) McLendon, G.; Miller J. R. J. Am. Chem. SOC. 1985, 107, 78 I 1-7816. (19) Conklin, K. T.; McLendon, G . J . Am. Chem. Soc. 1988, 110. 3345-3350. (20) Hazzard, J. T.; Poulos, T. L.; Tollin, G. Biochemisrry 1987, 26, 2836-2848. (21) Hazzard, J. T.; McLendon, G.; Cusanovich, M. A.; Tollin, G. Biochem. Biophys. Res. Commun. 1988, 151, 429-434. (22) Cheddar, G.; Meyer, T. E.; Cusanovich, M.A,; Stout, C. D.; Tollin, G.Biochemistry 1989, 28, 6318-6322.

0 1990 American Chemical Society

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Electron Transfer of Uroporphyrins and Cytochrome c Table I. Half-Cell Redox Potentials vs N H E (25 "C)

redox couple

cy10 c

L

AEO (V)

redox couple AEO (V) -0.43 cytc(IIl)/cytc(Il) 0.26O Fe"'Up(CI)/Fe"Up(CI) 0.806 -0.88b Zncvtc'JZncvtc Zncvtc+l'Zncvtc* 0.83< - 0 . 9 3 ~ ZnCp+/'znU+ ZnCp+/jZnUp* Up+/'Up* 0.86d -0.806 Up'/Up Reference 28. Reference 29.