Article pubs.acs.org/Biomac
Photoreversible Surfaces to Regulate Cell Adhesion Alexis Goulet-Hanssens,† Karen Lai Wing Sun,‡ Timothy E. Kennedy,‡ and Christopher J. Barrett*,† †
Department of Chemistry, Program in NeuroEngineering, McGill University, 801 Sherbrooke Street West, Montreal, Quebec, Canada H3A 0B8 ‡ Department of Neurology and Neurosurgery, Program in NeuroEngineering, Montreal Neurological Institute, 3801 University Street, Montreal, Quebec, Canada H3A 2B4 S Supporting Information *
ABSTRACT: We report the development of a photoreversible cell culture substrate. We demonstrate the capacity to modify the adhesivity of the substrate using light, altering its capacity to support cell growth. Polyelectrolyte multilayers (PEMs) were used to produce tunable substrates of different thickness and matrix stiffness, which have different intrinsic capacities to support cell adhesion and survival. Surfaces were top-coated with a poly(acrylic acid)-poly(allylamine hydrochloride) polyelectrolyte bilayer functionalized with a small fraction (
