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Plasma Surface Modification of POSS-PCU with Allylamine Enhances the Response and osteogenic Differentiation of Adipose-Derived Stem Cells Camilo Chaves, Feras Alshomer, Robert G. Palgrave, and Deepak M Kalaskar ACS Appl. Mater. Interfaces, Just Accepted Manuscript • DOI: 10.1021/acsami.6b05788 • Publication Date (Web): 07 Jul 2016 Downloaded from http://pubs.acs.org on July 13, 2016
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ACS Applied Materials & Interfaces
Plasma Surface Modification of POSS-PCU with Allylamine Enhances the Response and Osteogenic Differentiation of Adipose-Derived Stem Cells Camilo Chaves1, 2, Feras Alshomer1, Robert Palgrave3, Deepak M. Kalaskar1* 1
UCL Centre for Nanotechnology and Regenerative Medicine, Division of Surgery &
Interventional Science, University College London, London, United Kingdom, NW3 2PF. 2
Université Paris Sud, Orthopedic Surgery, Hôpital Saint Antoine, 184, rue du Faubourg-Saint-
Antoine, Paris, France, 75012. 3
Department of Chemistry, University College London, 20 Gordon Street, London, United
Kingdom, WC1H 0AJ. *Corresponding author: Dr Deepak M Kalaskar Lecturer in Nanotechnology and Cellular Engineering Centre for Nanotechnology and Regenerative Medicine Division of Surgery & Interventional Science Rowland Street, NW3 2PF University College London, London, United Kingdom. E-mail:
[email protected] Financial Disclosure: None of the authors have any commercial associations or financial relationships that would create a conflict of interest with the work presented in this article. Funding: This study was funded by Royal Free Charity (Award Number: 167275).
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ABSTRACT This study present amino functionalization of biocompatible polymer polyhedral oligomeric silsequioxane poly (carbonate-urea) urethane (POSS-PCU) using plasma polymerization process to induce osteogenic differentiation of adipose derived stem cells (ADSCs). Optimization of plasma polymerization process was carried out keeping cell culture application in mind. Thus, samples were rigorously tested for retention of amino groups under both dry and wet conditions. Physio-chemical characterization was carried out using ninhydrin test, X-ray photon spectroscopy, scanning electron microscopy and static water contact analysis. Results from physio chemical characterization shows that functionalization of amino group are not stable under wet condition and optimization of plasma process is required for stable bonding of amino groups to POSS-PCU polymer. Optimized samples were later tested in vitro in short and long term culture to study differentiation of ADSCs on amino modified samples. Short term cell culture shows that initial cell attachment was significantly (p
