Platinum Nanocatalyst Amplification: Redefining ... - ACS Publications

Pre-development a b. Seropositive. Free p24. < 3. < 3. 21.9. 99.7. > 125. > 125. 10. 4 < 3 < 3 pg·mL-1 c. Page 4 of 24. ACS Paragon Plus Environment...
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Platinum Nanocatalyst Amplification: Redefining the Gold Standard for Lateral Flow Immunoassays with Ultra-Broad Dynamic Range Colleen N. Loynachan, Michael R Thomas, Eleanor R Gray, Daniel A Richards, Jeongyun Kim, Benjamin S Miller, Jennifer Clare Brookes, Shweta Agarwal, Vijay Chudasama, Rachel A. McKendry, and Molly M. Stevens ACS Nano, Just Accepted Manuscript • DOI: 10.1021/ acsnano.7b06229 • Publication Date (Web): 07 Dec 2017 Downloaded from http://pubs.acs.org on December 7, 2017

Just Accepted

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ACS Nano is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

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1 2 3 4 Patient 5 Plasma 6 7 8 Lyophilized 9 Pre-catalysis Conjugates 10 11 12 13 Antibody-PtNC 14 conjugates 15 16 17b Nanobody-cysteine 18 mutation 19 20 21 22 23 24 BromopyridazinedionePEG8-biotin 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53

Colorless CN/DAB + H2O2

(i)

H2O2

Black Precipitate Oxidized CN/DAB

2 •OH

(ii)

Amplified lateral flow assay

Nanobodybiotin

Target analyte

Polystreptavidin

c Ultra-broad dynamic range of PtNC LFIA Catalytic amplification

PtNC LFIA

+

ELISA 4th gen. LFIA 10-1 100

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101 102 103 104 [Analyte] pg·mL-1

a

Pt4+

b ACS Nano

Pt4+

Au Pt4+ 1 2 Pt4+ 3PVP + 4 L-Ascorbic Acid 5 6 65 ℃ 7 8 9 PtNC 10 11 12 c 13 i14 15 16 17 18 19 20 21 22 100 nm 23 24 0.18 nm iii 25 26 27 0.22 nm 2 nm 28 29 30 31 32 33 50 nm 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53

d ii

iii iii

100 nm iv iv

e 311 220 200 111

ACS Paragon Plus Environment

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Page a 3 of 24 1 2 3 4 5 6 7 8 9 10 11 12b 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53

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a

1 2 3 4 5 6 7 8 9 10 11 12 b 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 c 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53

Post-development

ACS Nano

Pre-development

> 125

Free p24

Seropositive

> 125 99.7

pg·mL-1

21.9