Porous, Aligned and Biomimetic Fibers of Regenerated Silk Fibroin

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Porous, Aligned and Biomimetic Fibers of Regenerated Silk Fibroin Produced by Solution Blow Spinning Adrian Magaz, Aled D. Roberts, Sheida Faraji, Tatiana R. L. Nascimento, Eliton S Medeiros, Wenzhao Zhang, Ryan D. Greenhalgh, Andreas Mautner, Xu Li, and Jonny J. Blaker Biomacromolecules, Just Accepted Manuscript • DOI: 10.1021/acs.biomac.8b01233 • Publication Date (Web): 02 Nov 2018 Downloaded from http://pubs.acs.org on November 3, 2018

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

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Biomacromolecules

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Porous, Aligned and Biomimetic Fibers of Regenerated Silk

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Fibroin Produced by Solution Blow Spinning

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Adrián Magaz1,2†, Aled D. Roberts1†, Sheida Faraji1, Tatiana R.L. Nascimento3, Eliton

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S. Medeiros3, Wenzhao Zhang1, Ryan D. Greenhalgh1⊥, Andreas Mautner4, Xu Li2,5*,

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Jonny J. Blaker1*

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1

7

Manchester, United Kingdom

8

2

9

Technology and Research (A*STAR), Singapore

Bio-Active Materials Group, School of Materials, The University of Manchester,

Institute of Materials Research and Engineering (IMRE), Agency for Science,

10

3

11

Universidade Federal da Paraíba, João Pessoa, Brazil

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4

13

Research, University of Vienna, Vienna, Austria

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5

Laboratory of Materials and Biosystems, Department of Materials Engineering,

Polymer and Composite Engineering Group, Institute of Materials Chemistry and

Department of Chemistry, National University of Singapore, Singapore

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Present address: ⊥Department of Physiology, Development and Neuroscience,

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University of Cambridge, United Kingdom

17

18

†Equal author contribution *Corresponding authors:

Jonny J. Blaker

Li Xu

School of Materials

Institute

of

Materials

Research

and 1

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The University of Manchester

Engineering (IMRE)

Manchester, M13 9PL, UK

Singapore, 138634

tel. +44(0)161 3063578

tel. +65 64168933

e-mail: [email protected]

e-mail: [email protected]

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Abstract

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Solution blow spinning (SBS) has emerged as a rapid and scalable technique for the

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production of polymeric and ceramic materials into micro/nano- fibers. Here, SBS was

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employed to produce sub-micron fibers of regenerated silk fibroin (RSF) from Bombyx

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mori (silkworm) cocoons based on formic acid or aqueous systems. Spinning in the

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presence of vapor permitted the production of fibers from aqueous solutions, and high

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alignment could be obtained by modifying the SBS set-up to give a concentrated

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channeled airflow. The combination of SBS and a thermally induced phase separation

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technique (TIPS) resulted in the production of macro/micro- porous fibers with 3D

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interconnected pores. Furthermore, a co-axial SBS system enabled a pH gradient and

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kosmotropic salts to be applied at the point of fiber formation, mimicking some of the

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aspects of the natural spinning process, fostering fiber formation by self-assembly of the

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spinning dope. This scalable and fast production of various types of silk based fibrous

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scaffolds could be suitable for a myriad of biomedical applications.

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Keywords: Regenerated silk fibroin, solution blow spinning, fibers, porous fibers,

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aligned fibers, biomimetic spinning

36 37

1. INTRODUCTION

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Solution blow spinning (SBS) offers an efficient method to produce micro/nano- fibers 2

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at a significantly higher rate (about ×100 times faster

) compared to conventional

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electrospinning. By using pressurized gas as the driving force,2–5 it overcomes some of

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the drawbacks of electrospinning including the use of electric fields and dense fibrous

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networks with low porosities;6–9 in addition, fibers can be deposited in situ on virtually

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any surface.4 To date, a myriad of polymers,3–5 composites 1,10,11 and ceramics 12,13 have

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been successfully processed into fibers by SBS. The use of micro/nano- fibers is of

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particular interest in the field of regenerative medicine due to their high surface area to

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volume ratio and morphological resemblance to the extracellular matrix of native

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tissues. Fibers can provide adequate topographical and mechanical cues to direct cell

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behavior, making them suitable for the fabrication of scaffolds for tissue regeneration.14–

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16

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In its native state, Bombyx mori silk consists of fibrous protein filaments (silk fibroin)

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surrounded by a glue-like layer (sericin). Among the wide range of natural materials,

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silk fibroin has many attractive chemical, physical, and biological properties which

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make it convenient for regenerative medicine, tissue engineering and therapeutic

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delivery applications.17,18 Some of these properties include suitable cell-material

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interactions, tailored biodegradability and oxygen/water permeability.19–22

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Under natural spinning conditions, as the silk dope flows from the posterior to the

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anterior division of the silkworm gland, the dope is sheared, water content decreases

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from 80 to 77%, pH decreases from 6.9 to 4.8 and chaotropic ions are exchanged for

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kosmotropic ions,23–29 contributing to fiber formation. Silk fibroin typically requires

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reprocessing from its native state to tune properties including fiber diameter and surface

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roughness/morphology to suit the intended application15,30. In particular, regenerated

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silk fibroin (RSF) from aqueous solutions has previously been electrospun by blending

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with high molecular weight poly(ethylene oxide) (PEO),21,31,32 used as a temporarily 3

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water-soluble spinning aid, and fully aqueous processes based on high molecular weight

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silk fibroin dopes have been successfully produced via electrospinning.33,34 Recently,

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straining flow spinning (SFS) has been used to produce high performance RSF fibers by

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controlling the flow of the dope through its interaction with a coagulant bath

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mimicking the balanced mild aqueous chemical environment and moderate shear forces

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found in natural spinning glands.35 While robust RSF fibers can be mass-produced by

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SFS, these range between 8-13 μm in diameter. SBS permits faster production rates (5

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μL.min−1 in SFS compared with >100 μL.min−1 in SBS) and can even achieve thinner

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fibers at the nanoscale.

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The aim of this study is not to produce high performance robust fibers nor retain the

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hierarchical structures of natural silks, but rather show that SBS is a suitable technique

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that could be used to mass-produce various types of RSF sub-micron fibers with

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potential applications in regenerative medicine. Here, to the author’s knowledge we

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have for the first time employed SBS to process RSF into randomly aligned and aligned

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non-porous micro/nano- fibers, utilizing both organic (formic acid) and aqueous solvent

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systems, channeling the flow of air to the collector. Porous fibers from an aqueous

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system were obtained by using a modified SBS system based on a thermally induced

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phase-separation (TIPS) technique.1,37 A co-axial SBS system was also employed to

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mimic a pH gradient as a means to replicate some of the physiological parameters of the

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natural spinning process26 and drive secondary protein structure formation.

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2. EXPERIMENTAL SECTION

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2.1 Regenerated silk fibroin

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RSF solutions from Bombyx mori silkworm cocoons (Wildfibres; Birmingham, UK)

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were prepared following a previously described protocol

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Briefly, cocoons were dewormed and degummed in a 0.02 M boiling Na2CO3 (Sigma-

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35,36

,

with minor modifications.

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Aldrich, UK) aqueous solution for 30 min to remove the sericin. Degummed silk fibroin

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was thoroughly washed in deionized water (three water changes, 20 min each) and air-

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dried for 24h. The dried degummed silk fibroin was dissolved in 7.9 M aqueous LiBr

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(Sigma-Aldrich, UK) to form a 16% w/v solution, with gentle continuous stirring at

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60°C for 4h. The solution was centrifuged (10,000 ×g, 20 min) to remove any residual

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pupa and dialyzed (3.5kDa MWCO SnakeSkin, ThermoScientific, UK) against 5L of

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deionized water at 4°C for 3 days, with regular water changes under continuous stirring.

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The concentration of the RSF solution after water dialysis was ~5-6% w/v. The solution

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was centrifuged again (10,000 ×g, 20 min), and either freeze-dried for 72h (VirTis

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BenchTop Pro; New York, USA) or concentrated for further use, described below.

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2.2 Spinning dope formulations

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Formic acid dopes as precursors of non-porous fibers were prepared by dissolving

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freeze-dried RSF in formic acid (95% v/v; Sigma-Aldrich, UK) at a concentration of 8-

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20% w/v at room temperature and stirred for 2h prior to spinning.

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Aqueous precursor dopes were prepared by means of reverse dialysis processes.

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Aqueous formulations as precursors for non-porous fibers were prepared by

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concentrating an RSF solution by reverse dialysis (10 kDa MWCO SnakeSkin dialysis

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tubing, Thermo-Scientific, UK) against a 15% w/v polyethylene glycol (PEG 10,000

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Mw; Sigma-Aldrich, UK) aqueous solution at 4°C. Note that some PEG likely diffused

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into the RSF solution and may have acted as a spinning aid. Prior to spinning, a 3M

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CaCl2 (Sigma-Aldrich, UK) solution was added into the RSF system to adjust the [Ca]2+

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to 0.3 M (~pH 7.5, 9:1 v/v ratio SF/CaCl2). The role of CaCl2 is to promote RSF

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conformation from random coil/α-helix to β-sheet.39–41 The pH was subsequently

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adjusted to 10.5 with a 5M NaOH (Sigma-Aldrich, UK) solution, which was found to

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make the dope stable during the spinning process and promote fiber formation. Aqueous 5

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formulations as precursors of porous fibers and biomimetic fibers were prepared by

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concentrating an RSF solution by reverse dialysis (3.5 kDa MWCO SnakeSkin dialysis

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tubing, Thermo-Scientific, UK) against a 20% w/v PEG (10,000 Mw; Sigma-Aldrich,

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UK) aqueous solution at 4°C.

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All solutions were stored in sealed glass vials at 4ºC and spun within 24h.

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2.3 Rheological analysis of regenerated silk fibroin solutions

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RSF formulations at increasing concentrations were loaded into an AR-G2 rheometer

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(TA Systems; UK), operated with a parallel plate geometry (20 mm diameter and gap

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set to 600 μm) at 25°C and with an environmental case attached to prevent solvent

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evaporation. Each concentration (n=2 replicates of the same solution) was subjected to a

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logarithmic steady shear rate increase from 0.01 to 1000 s-1.42

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2.4 Fiber production by conventional, cryogenic and co-axial solution blow

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spinning

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All RSF dopes were spun via a custom made SBS set-up (Scheme 1), consisting of a

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syringe pump, connective tubing, a concentric nozzle and a compressed air source

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controlled by a pressure regulator. Solutions were injected to the inner nozzle of the

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SBS head using a precision syringe driver pump (N-300, New Era Pump Systems Inc.;

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USA). Compressed air was delivered to the outer nozzle via an oil-free and water-free

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air compressor (Bambi VT150D; Italy). The internal diameter (ID) of the SBS inner

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nozzle was 0.8 mm, the outer diameter (OD) was 1.8 mm; the outer nozzle had an ID of

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2 mm and an OD of 3 mm. The inner nozzle was set to protrude by ≥ +2 mm from the

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outer nozzle into a conical shaped end.

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Random non-porous fibers from RSF formulations based on formic acid were spun

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directly with a conventional SBS apparatus (Scheme 1 a-b): feed rate of 50-60 6

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μL.min−1, air pressure of 30-40 psi (0.21-0.28 MPa) and working distance to the

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collector of 20-30 cm. Production of random non-porous fibers from RSF aqueous

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dopes was enhanced by spinning through a vapor immediately after the SBS nozzle.

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Vapor was generated using different solvents (water, absolute ethanol or absolute

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methanol) in a beaker on a hotplate (100-200°C) placed under the SBS nozzle. Ethanol

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and methanol are known to promote β-sheet formation. Spinning parameters in this case

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were: feed rate of 100-110 μL.min−1, air pressure of 30-60 psi (0.21-0.41 MPa) and

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working distance of 30-40 cm to fiber collection.

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Aligned non-porous fibers out of formic acid and aqueous systems were obtained by

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placing a funnel between the SBS head and collector (Scheme 1c). This served to

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channel the airflow and the fiber forming jet, aligning the fibers in flight prior to

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collection. The same spinning parameters as previously stated for non-porous fibers

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were used.

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Porous fibers were obtained using RSF aqueous dopes based on a cryogenic SBS (cryo-

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SBS) set-up (Scheme 1d).1,37 As-produced RSF aqueous formulations were sprayed into

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fibers directly into a bath of liquid nitrogen. The feed rate was 100 μL.min−1 and the air

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pressure 30 psi (0.21 MPa); a working distance of 15 cm and an angle of ~45° to the

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cryogenic bath were selected to limit liquid nitrogen loss. The bath container was made

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from an expanded-polystyrene foam box. During cryo-SBS, the level of liquid

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nitrogen in the bath was constantly topped up to ensure that the working distance

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remained ±2 cm of the target value. Frozen fibers were then collected into 50 mL

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Falcon® tubes, transferred to lyophilization flasks (pre-cooled in liquid nitrogen) and

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subsequently lyophilized (VirTis BenchTop Pro; New York, US). The external glass

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portion of the lyophilization flask was kept under liquid nitrogen using the polystyrene

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foam box until a vacuum of ~50 mTorr was reached, after which the outside of the flask 7

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was exposed to ambient conditions. Lyophilization was continued for a total of 72h to

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ensure complete drying. a

Vapor phase Pressure gauge

Pressurized gas

b Air nozzle

Collector

SF solution

Nozzle

Pressurized air

Solution cone

Polymer jet Fiber

Solution pump

c

d

Pressure gauge

Pressure gauge Funnel

Nozzle

Cryogenic bath

Nozzle

e

Air nozzle Outer solution nozzle Inner solution nozzle SF solution

Pressurized air

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f

Air nozzle Inner solution nozzle Outer solution nozzle

SF solution Outer solution Self-assembly

Pressurized air

Self-assembly

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Scheme 1 Schematic representation of conventional, cryogenic and co-axial SBS

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set-ups. (a-b) Conventional SBS set-up (with/without vapor phase). (c) Modified SBS

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set-up to channel the airflow and RSF fiber jet for spinning aligned non-porous fibers.

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(d) Cryo-SBS set-up for the production of porous fibers. (e-f) Co-axial SBS set-up to

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induce a pH-ion gradient by (e) positive and (f) negative inner nozzle solution

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protrusion.

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For biomimetic fibers, a co-axial SBS head, consisting of two concentric solution

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nozzles (inner solution nozzle, outer solution nozzle) within an outer pressurized air

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nozzle, was employed to induce changes to the chemical environment at the point of the 8

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solution cone (positive protrusion of inner solution nozzle, Scheme 1e) or prior to it

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(negative protrusion of inner solution nozzle, Scheme 1f) by flowing a secondary

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solution through the outer solution nozzle whilst flowing the RSF solution through the

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inner solution nozzle. The outer solution nozzle was set to protrude ≥ 2 mm from the

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outer pressurized air nozzle; the inner solution nozzle was set to protrude ± 2 mm

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(positively or negatively) from the outer solution nozzle. A number of kosmotropic

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phosphate solutions adjusted to various acidic pH values (pH 2-7) and a commonly used

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coagulant in wet-spinning (ammonium sulfate)

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aqueous RSF solutions into fibers. Two precision syringe driver pumps were used for

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injecting the inner and outer solutions. Successful fiber spinning was achieved with the

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following set parameters. For the positive nozzle protrusion, inner and outer solutions

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were injected at feed rates 100 μL.min−1 and 150 μL.min−1, respectively; for the

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negative nozzle protrusion, the inner and outer solutions were injected at feed rates of

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190 μL.min−1 and 100 μL.min−1, respectively. Air pressure and distance to collector

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were maintained in both cases at 40 psi (0.28 MPa) and 40 cm.

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2.5 Insolubilization of solution blow spun regenerated silk fibroin fibers

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Non-woven random fiber mats were immersed in 90% v/v ethanol (EtOH) or 90% v/v

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methanol (MeOH) baths for 10 min to further induce protein secondary structure

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transition from α helix/random coils into β-sheets. The mats were then dried in an oven

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at 30°C for 24h.

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2.6 Physico-mechanical characterization of the fibers

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2.6.1 Fiber morphology

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Fiber morphology was assessed using scanning electron microscopy (SEM) on a Hitachi

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S300 N (USA) and on a PhenomPro Generation 5 (USA), and field emission scanning

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electron microscopy on a Zeiss Supra 35VP FE-SEM (Germany). Briefly, fiber mats

22

was used to induce self-assembly of

9

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(1cm x 1cm) were mounted onto aluminium stubs with double-sided conductive carbon

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tape and sputter-coated with gold/palladium (Gatan Model 682 Precision Etching

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Coating System, USA) to an average thickness of 3 nm; images were captured at 2.5-5

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kV. Average fiber diameter was determined using ImageJ (Fiji 1.28, USA) software, by

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measuring a minimum of 50 fibers per sample type.

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2.6.2 Protein structure quantification and crystallinity assessment

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The conformational structure of non-woven RSF fiber mats was investigated by wide-

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angle x-ray diffraction (WAXD; PANalytical X’Pert Pro, UK) with the following

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parameters: copper line focus X-ray tube with Ni kβ absorber (0.02 mm; Kβ=1.392250

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Å) and Kα radiation (Kα1=1.540598 Å, Kα2=1.544426 Å, Kα ratio 0.5, Kαav=1.541874

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Å), diffraction angle of 5-45° and scanning rate of 2°.min−1. In addition, a Fourier

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with an iD5 attenuated total reflectance (ATR) attachment (diamond crystal) was used to

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examine the conformational changes of the secondary structure of RSF within the amide

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I region. For each measurement, 32 scans were recorded with a resolution of 4 cm-1 and

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wavenumbers from 500 to 6000 cm-1. The amide I region ranging from 1600 to 1700

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cm-1 was baseline corrected and deconvoluted by applying a second derivative

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algorithm, a signal smoothing algorithm and a curve-fitting algorithm based on Fourier

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Self-Deconvolution (FSD) according to references

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at 1595 cm−1 (side chains), 1610 cm−1 (side chains), 1620 cm−1 (intermolecular β-

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sheets), 1630 cm−1 (intermolecular β-sheets), 1640 cm−1 (random coils), 1650 cm−1

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(random coils), 1660 cm−1 (α helices), 1670 cm−1 (β-turns), 1680 cm−1 (β-turns), 1690

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cm−1 (β-turns) and 1700 cm−1 (intramolecular β-sheets) following reference 45. Samples

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were run in triplicate.

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2.6.3 Mechanical testing

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transform infrared spectrometer (FTIR Nicolet iS5; Thermo Scientific, UK) equipped

43,44

. The band positions were fixed

10

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Uniaxial tensile testing of as-spun RSF mats (cardboard window frame dimensions of 5

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mm x 5 mm) of aligned non-porous fibers was performed with a mechanical tester

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(Instron 3344; Instron Ltd., USA). The ends of the fiber mats were embedded in epoxy

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and taped to avoid stress concentration at the clamps during mounting to the tensile test

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machine. Prior to testing, the sides of the window frame were cut through, enabling

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direct loading of the sample. Samples (n=12 replicates) were subjected to uniaxial loads

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at 2 mm.min−1, a 10N load cell was used. The strain at break and ultimate tensile

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strength (UTS) were calculated from the maximum value of the stress-strain curve

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before breakage, Young’s modulus from the slope of the linear portion of the curve, and

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toughness from the area under the graph for each sample tested. The area occupied by

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the fibers was calculated by measuring the area of the mat and subtracting the void

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space of the fibers based on mass-density differences between the mat and single

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fibers.46 Instruments were calibrated prior to use and all tests were performed at room

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temperature.

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2.6.4 Surface area analysis and fiber pore size

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Brunauer–Emmett–Teller (BET) surface area measurements of porous fibers obtained

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by cryo-SBS were made using a surface area and porosity analyzer (TriStar II,

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Micromeritics, Neu-Purkersdorf, Austria). Prior to gas adsorption experiments,

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impurities were removed via a degassing step (FlowPrep 060, Micromeritics).

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Approximately 100 mg of sample was placed inside a glass chamber, purged with

245

nitrogen at ambient temperature overnight. Nitrogen adsorption isotherms were then

246

measured at 77 K.

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The size of a minimum of 70 pores from SEM images of porous fibers was measured

248

using by ImageJ (Fiji 1.28, USA); measurements were performed in the horizontal

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direction through the center of the pore. 11

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2.7 Data analysis

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Quantitative data is presented as standard deviation (SD) of the mean values. Statistical

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analysis of the results was performed using GraphPad Prism 7 (San Diego, USA).

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3. RESULTS AND DISCUSSION

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3.1 Non-porous RSF fibers obtained via SBS

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Analysis of the viscoelastic properties of RSF formulations is of interest since solution

256

viscosity determines whether a droplet spray or continuous fibers are obtained, as it

257

affects initial droplet shape and stability of the jet trajectory during SBS.47 The viscosity

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measurements of RSF formulations of formic acid and the aqueous system at increasing

259

concentrations are shown in Figure 1 a-b. Both systems exhibited slightly different

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rheologies over the range of concentrations tested. For the case of RSF solutions in

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formic acid, the viscosities were almost independent of the shear rate exhibiting

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Newtonian-like behavior. This is consistent with work reported by Zhu et al. for RSF

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formulations in formic acid below 20% w/v.48 Aqueous RSF formulations, on the other

264

hand, exhibited non-Newtonian shear-thinning-like behavior, more evident at high

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solution concentrations. Shear-thinning at increasing shear rate is characteristic of

266

native silk dopes49 and suggests more entanglement of the chain networks in the

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polymer solution,50,51 which should aid spinning by SBS.

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The solutions over the range of different concentrations were spun via conventional

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SBS. A systematic investigation found that non-porous fibers only formed for

270

concentrations between 10-18% w/v for solutions based on the formic acid system, and

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between 25-30% w/v for aqueous solutions in the presence of a water/ethanol/methanol

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vapor and pH-Ca2+ adjustment as detailed in the experimental section. Solutions lower

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than 10% and above 18% w/v (formic acid system), or below 25% and above 30% w/v

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(aqueous system), did not have suitable rheological properties for processing into fibers 12

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by SBS: either the nozzle got blocked frequently or the solutions resulted exclusively in

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droplets and no fibers were formed. The spinnability windows (Figure 1 c-d) for RSF

277

solutions were established as 15-144 mPa.s (formic acid system) and 500-1500 mPa.s

278

(aqueous system), identified as the target viscosities for blow spinning RSF dopes into

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non-porous fibers based on formic acid and aqueous systems respectively. a

b

102

Viscosity (Log[Pa.s])

Viscosity (Log[Pa.s])

101 100 10-1 10-2 10-3 100

101

102

102 101 100 10-1 10-2 10-3 100

103

101

Shear rate (Log[1/s])

c

102

103

Shear rate (Log[1/s])

d

2

5x10

3x103 2

4x10

Viscosity (mPa.s)

Viscosity (mPa.s)

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

Biomacromolecules

3x102 2x102 1x102

2x103

1x103

0

0

5

280

281

10

15

20

5

SF concentration (% w/v)

10

15

20

25

30

35

SF concentration (% w/v)

5% w/v

7% w/v

8% w/v

10% w/v

11% w/v

12% w/v

15% w/v

16% w/v

17% w/v

20% w/v

21% w/v

25% w/v

27% w/v

32% w/v

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Figure 1 Rheology of RSF solutions. (a-b) Shear sweep measurements of (a) formic

283

acid and (b) aqueous RSF solutions at increasing concentrations. Red arrows represent

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increase in concentration. (c-d) Relationship between viscosity (at 1000 s-1 shear rate)

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and concentration for (c) formic acid and (d) aqueous solutions, highlighting the

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spinnability window for SBS; error bars in SD (n=2). 13

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Page 14 of 39

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SEM images of RSF non-porous fibers spun from formic acid are shown at 12 and 15%

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w/v (Figure 2 a-b, Figure S1). The most stable fiber mats were obtained at a flux of 50-

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60 μL.min−1 at an air pressure of 30-40 psi (0.21-0.28 MPa) with a working distance of

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20-30 cm to fiber collection. Increasing the concentration of the RSF formulation

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resulted in fibers with a greater diameter consistent with previously reported data of

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solution blow spinning of other polymers.3,52,53 The mean diameter of randomly aligned

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fibers based on formic acid (Figure 2a, Figure S1a) was 290 (±60) nm and 910 (±300)

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nm for RSF solution concentrations at 12 and 15% w/v, respectively. Fiber morphology

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was relatively smooth in both cases. Aligned fiber bundles and mats were also produced

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at 12% w/v (Figure 2b, Figure S1b) by channeling the airflow towards the collector.

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Fibers were quickly formed as the RSF solution exited the nozzle and consolidated

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within less than 5 cm, meaning that by channeling the flow through greater distances

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fiber alignment could be achieved. Aligned non-porous fibers exhibited a similar mean

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fiber diameter (p-value non-significant) compared to that of randomly aligned non-

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porous fibers produced from RSF formulations at the same concentration. The mean

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fiber diameter in this case was 260 (±150) nm, and fiber morphology was smooth and

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consistent, with no droplets/beads observed.

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Biomacromolecules

304 305

Figure 2 SEM images of RSF non-porous fibers from formic acid and aqueous

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solutions. (a-b) Fibers spun from formic acid at 12% w/v: (a) randomly aligned fibers;

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(b) aligned fiber bundle with inset at higher magnification. (c-d) Fibers spun from an

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aqueous system at 27% w/v through a water vapor: (c) randomly aligned fibers; (d)

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aligned fibers.

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For aqueous RSF solutions, the most stable non-porous fibers were obtained at a flux of

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100-110 μL.min−1 at an air pressure 30-60 psi (0.21-0.41 MPa) and working distance of

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30-40 cm. SEM images of RSF fibers spun at 27 w/v % are shown in Figure 2 c-d. The

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structure of the fiber mats consists of non-porous fibers interconnected into a 3D porous

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non-woven network with some conglutination; with a mean diameter of 410 (±130) nm

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for randomly aligned fibers (Figure 2c) and 465 (±170) nm for aligned fibers (Figure

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2d) (p-value non-significant between them). Compared to RSF fibers produced from 15

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Page 16 of 39

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formic acid dopes (Figure 2 a-b), these fibers had poorer morphological homogeneity,

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which can be attributed to differences in the solvent volatility. Water has a relatively

319

high boiling point and low volatility, resulting in residual moisture content prior to

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collection.54 In contrast, formic acid is highly volatile, meaning fibers are readily

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formed as the RSF jets through the SBS nozzle.

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It should be noted that some low molecular weight PEG likely diffused into the aqueous

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RSF solutions during reverse dialysis concentration, and that this acted as a spinning aid

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during SBS promoting fiber formation. A plasticizing effect due to the presence of PEG

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in polylactide (PLA) blends has been reported,55 considerably increasing the elongation

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at break, while the tensile strength remained unchanged. Low molecular weight PEG is

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difficult to spin on its own,56,57 and only its high molecular weight form, PEO, is

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commonly used during electrospinning of aqueous biopolymer systems due to its higher

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viscosity that aids stabilizing the polymer jet.

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PEG is water-soluble and can be easily removed after fiber spinning. RSF solutions for

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aqueous non-porous fiber spinning were also concentrated up to 30% w/v by forced air-

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flow and by reverse dialysis in PEG using a 3.5 kDa membrane, yet none of the

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prepared solutions were able to form fibers, suggesting that concentrations >30% w/v

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may be required. However, this is disadvantageous for industrial processing due to the

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easy transition of highly concentrated solutions into gel and clogging of the nozzle.

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The structure of non-porous fibers was further characterized by ATR-FTIR and WAXD

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(Figure 3 a-c). Indeed, the conformation and structure of proteins are an important

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factor in both their physico-chemical and mechanical properties, which ultimately

339

affects their ability to interact with biological interfaces – an important consideration for

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tissue engineering and therapeutic delivery applications. 16

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Biomacromolecules

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The ATR-FTIR spectra of the amide I (1700-1600 cm−1) and amide II (1600-1450 cm−1)

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bands of non-porous fibers spun from formic acid and aqueous solutions are shown in

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Figure 3a, indicating that the typical chemical composition of silk fibroin remained

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unaffected during SBS. A clear shift of the absorption peak of the amide I region (1700-

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1600 cm−1) after post-treatment in ethanol and methanol can be observed.

346 347

A detailed quantification of the amide I band by curve fitting and deconvolution

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revealed changes in the chain structure (Figure 3c). While β-sheets make up the

349

crystalline regions of silk fibroin, other more flexible structures such as β-turns or

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random coils and α-helices account for the more amorphous structures of silk.58,59

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Significantly greater (p