Preparation and characterization of cobalt (III)-and chromium (III

Sandra S. Eaton , Gareth R. Eaton. Coordination Chemistry Reviews 1988 83, 29- ... Moshe M. Werber , Amos Lanir. Israel Journal of Chemistry 1981 21 (...
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BALAKRlSHhAW 4YD VILLAFRANCA

Preparation and Characterization of Cobalt( 111)- and Chromium(II1)-Glutamine Synthetase Derivativest M. S. Balakrishnan and Joseph J. Villafranca*

ABSTRACT:Stoichiometric incorporation of Co(II1) into the n, metal ion sites of Escherichia coli glutamine synthetase (unadenylylated) was carried out by hydrogen peroxide oxidation of enzyme-bound Co(I1). This enzyme derivative was found to bind 1 mol of Mn(I1) per subunit to the n2 metalnucleotide sites with a KD value of 0.67 0.1 1 m M . The Co(II1)-enzyme was catalytically inactive (