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Pu-erh tea extract attenuates nicotine-induced foam cell formation in primary cultured monocytes: an in-vitro mechanistic study Shih-Hsin Tu, Ming-Yao Chen, Li-Ching Chen, Yi-Ting Mao, Chi-Hou Ho, WenJui Lee, Yen-Kuang Lin, Min-Hsiung Pan, Chih-Yu Lo, Chi-Long Chen, Yun Yen, Jacqueline Whang-Peng, Chi-Tang Ho, Chih-Hsiung Wu, and Yuan-Soon Ho J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.6b00624 • Publication Date (Web): 21 Mar 2016 Downloaded from http://pubs.acs.org on March 31, 2016
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Journal of Agricultural and Food Chemistry 1
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Title
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Pu-Erh tea extract attenuates nicotine-induced foam cell formation in primary cultured monocytes:
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an in vitro mechanistic study
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Authors
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Shih-Hsin Tu&,#,¢, ¥, †; Ming-Yao Chen§; Li-Ching Chen§, £†; Yi-Ting Mao¶; Chi-Hou Ho¶; Wen-Jui
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Lee¶; Yen-Kuang Lin©; Min-Hsiung Pan®; Chih-Yu Lo¤; Chi-Long Chen◎,○; Yun Yen&,¢; Jacqueline
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Whang-Peng&,¢; Chi-Tang Ho@; Chih-Hsiung Wu&,#,"* and Yuan-Soon Ho&,¶,!,¦*
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Affiliations
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&
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#
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Taiwan
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¢
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¥
Breast Medical Center, Taipei Medical University Hospital, Taipei, Taiwan
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§
Division of Gastroenterology, Department of Internal Medicine, Shuang Ho Hospital, Taipei
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Medical University
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£
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Taiwan
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¶
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Technology, Taipei Medical University, Taipei, Taiwan
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©
Comprehensive Cancer Center of Taipei Medical University, Taipei, Taiwan
Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei,
Taipei Cancer Center, Taipei Medical University, Taipei, Taiwan
Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei,
School of Medical Laboratory Science and Biotechnology, College of Medical Science and
Biological Statistics and Research Consultation Center, Taipei Medical University, Taipei, Taiwan
ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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®
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¤
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◎Department
of Pathology, Taipei Medical University Hospital, Taipei, Taiwan
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○Department
of Pathology, College of Medicine, Taipei Medical University, Taipei, Taiwan
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@
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"
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!
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Taiwan
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¦
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†Dr. Li-Ching Chen and Dr. Shih-Hsin Tu contributed equally to this manuscript.
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*Correspondence:
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Yuan-Soon Ho, Ph.D., Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical
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University, No. 250 Wu-Hsing Street, Taipei 110, Taiwan. Phone: +886-2-2736-1661 ext. 3420; Fax:
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+886-2-2739-3422; E-mail:
[email protected]. Chih-Hsiung Wu, M.D., Ph.D., Department of
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Surgery, School of Medicine, College of Medicine, Taipei Medical University, No. 250 Wu-Hsing
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Street, Taipei 110, Taiwan. Phone: +886-2-2249-0088 ext. 8810; Fax: +886-2-2739-3422; E-mail:
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[email protected].
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Keywords: tea components, atherosclerosis, foam cell, α9-nicotinic acetylcholine receptor
Institute of Food Sciences and Technology, National Taiwan University, Taipei, Taiwan
Department of Food Science, National Chiayi University, Chiayi City, Taiwan.
Department of Food Science, Rutgers University, New Brunswick, New Jersey 08901, USA
Department of Surgery, En Chu Kong Hospital, New Taipei City 237, Taiwan
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei,
Department of Laboratory Medicine, Taipei Medical University Hospital, Taipei, Taiwan
ACS Paragon Plus Environment
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Abstract
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In this study, the mechanisms by which Pu-Erh tea extract (PETE) attenuates nicotine-induced foam
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cell formation were investigated. Monocytes were purified from healthy individuals using commercial
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antibodies coated with magnetic beads. We found that the nicotine-induced (1-10 µM) expression of
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oxidized low-density lipoprotein receptors (ox-LDLRs) and α9-nAchRs in monocytes was
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significantly attenuated by 24 hr of PETE (10 µg/mL, *p1 µg/mL for 24 hr, *p
