Purification of His-tagged Proteins with [Desthiobiotin-BSA-EDTA

Purification of His-tagged Proteins with [Desthiobiotin-BSA-EDTA] Conjugates Exhibiting Resistance to EDTA. Guy Patchornik. Bioconjugate Chem. , 2008,...
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Bioconjugate Chem. 2008, 19, 1112–1112

CORRECTIONS Volume 19, Number 3, 2008. Guy Patchornik Purification of His-tagged Proteins with [DesthiobiotinBSA-EDTA] Conjugates Exhibiting Resistance to EDTA The author regrets an error in the Methods section. On page 675, under General Purification Protocol for His6-Tagged Proteins Utilizing DB-BSA-EDTA Conjugates, the text “Step IV. Elution: The His-tagged protein was eluted from the pellet into 50–100 µL of 50 mM imidazole and 100 mM Tris pH 7 within 5–10 min” should read “Step IV. Elution: The His-tagged protein was eluted from the pellet into 50–100 µL of 50 mM imidazole and 50 mM Tris pH 8 within 5–10 min”. BC800123J 10.1021/bc800123j Published on Web 05/01/2008