The LC-UV Detector I Reference compensated system Reliable high sensitivity measurements can be made due to the low noise and drift characteristics of the detector. These characteristics are achieved by using a unique reference compensated system. Light emerging from the monochromator is divided by a beam splitter, a small percentage being reflected onto a reference photocell. T h e output of this reference photocell is amplified and used to electrically compensate for intensity changes in the arc of the deuterium lamp. £- Coated optics Optical components are coated with a thin layer of silica ensuring that their high standard of performance is maintained for year after year. Ο Dark current compensation Compensation for photocell dark current can, by simple screwdriver adjustment, easily be made by the customer to ensure the highest longterm stability.
*l· Standby Heater This low-power heater can be left on when the lamp is not in use, thus maintaining the electronics at a constant temperature and reducing instrument warm-up time. Ο Flow cell Designed for high performance this low volume temperature equilibrated flow cell is simplicity itself to dismantle for cleaning and to reassemble. Adjustment for maximum energy throughput is achieved by a single screwdriver operation with readout on to the recorder.
The wavelength range of the LC-UV detector allows an extremely wide variety of compounds to be detected, including those in the far U V around 200nm. Detector sensitivity can be maximised by choosing the optimum wavelength for a particular analysis or selectivity increased by using a wavelength at which interfer ing impurities do not absorb. The visible range accessory can be added to the detector in order to extend the wavelength range to 600nm thus catering for such important application areas as the analysis of food and dyes tuffs.
PROPYL PARABEN
Ό Switchable Filter Baseline noise can be minimised by means of an active filter which can operate with a time constant of 0.5,1 or 2 seconds, selected by a push-button control on the front panel.
METHYL PARABEN SORBIC A C I D INJECTION 7μΙ M E T H A N O L EXTRACT OF DOG FOOD 0.08 A U F S
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Dog food analysis - 200nm Careful selection of detector wavelength allows the analysis of sorbic acid together with methyl and propyl paraben in a complex food extract. Column: Partisil-10 ODS Mobile Phase : Water Acetonitrile Perchloric Acid 89.7 10 0.3
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BLUtVRSDYE CARMOSINE INJECT
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Mixture of Dyes tuffs - 600nm At 6oonm Blue VRS dye can be readily analysed in the presence of Carmosine. Column: Partisil-10 ODS Mobile phase : Methanol 0.1 M potassium dihydrogen phosphate, 0.1 M disodium hydrogen phosphate + 0.01 M tetrabutyl ammonium hydroxide 50 50.
The LC-UV Detector is just part of Pye Unicam's complete capability in LC — a capability that includes complete systems, modules, columns and accessories. For further information contact Pye Unicam or use the Reader Reply Service No. 170
Pye Unicam Ltd York Street Cambridge CB1 2PX England Telephone (0223)58866 -Telex817331 Printed in England by Lund Humphries 9/78 7042-05-2895-H
