3206
J. Agric. Food Chem. 1999, 47, 3206−3209
Rapid Photometric Assay Evaluating Antioxidative Activity in Edible Plant Material Jen-Min Kuo,*,† Dong-Bor Yeh,† and Bonnie Sun Pan‡ Department of Food Health, Chia-Nan College of Pharmacy and Science, Tainan, Taiwan, Republic of China, and Department of Marine Food Science, National Taiwan Ocean University, Keelung, Taiwan, Republic of China
The objective of the present study is to develop a rapid and convenient method to determine antioxidative activity. It was determined by the inhibition capacity on the hemoglobin-catalyzed peroxidation of linoleic acid. The appropriate conditions for reaction of 4 mM linoleic acid were 0.002% hemoglobin at 37 °C for 10 min. Adding methanol to the reaction mixture at
