Rapid Veterinary Diagnosis of Bovine ... - ACS Publications

Jan 11, 2018 - ABSTRACT: The health and well-being of cattle is an important issue in maintaining and increasing global .... After amplification, the ...
0 downloads 12 Views 2MB Size
Subscriber access provided by READING UNIV

Article

Rapid Veterinary Diagnosis of Bovine Reproductive Infectious Diseases from Semen using Paper-Origami DNA Microfluidics Zhugen Yang, Gaolian Xu, Julien Reboud, Syed Atif Ali, Gurpreet Kaur, John McGiven, nongthombam boby, Praveen Gupta, Pallab Chaudhuri, and Jonathan Mark Cooper ACS Sens., Just Accepted Manuscript • DOI: 10.1021/acssensors.7b00825 • Publication Date (Web): 11 Jan 2018 Downloaded from http://pubs.acs.org on January 12, 2018

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

ACS Sensors is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

Page 1 of 8 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60

ACS Sensors

Rapid Veterinary Diagnosis of Bovine Reproductive Infectious Diseases from Semen using Paper-Origami DNA Microfluidics Zhugen Yang1, Gaolian Xu1, Julien Reboud1, Syed Atif Ali2, Gurpreet Kaur2, John McGiven3, Nongthombam Boby4, Praveen K Gupta4, Pallab Chaudhuri2*, Jonathan M. Cooper1*. 1

Division of Biomedical Engineering, School of Engineer, University of Glasgow, Oakfield Avenue, Glasgow G12 8LT, United Kingdom 2 Division of Bacteriology, Indian Veterinary Research Institute, Izatnagar-243122, India 3 FAO/WHO Collaborating Centre for Brucellosis, OIE Brucellosis Reference Laboratory, Department of Bacteriology, Animal & Plant Health Agency, Woodham Lane, Addlestone, Surrey KT15 3NB, United Kingdom 4 Division of Biotechnology, Indian Veterinary Research Institute, Izatnagar-243122, India Keywords. isothermal amplification, paper, origami, infectious disease, diagnosis. ABSTRACT. The health and well-being of cattle is a significant concern for global agricultural output. In dairy production within low and middle income countries (LMICs), there is a significant biosensing challenge in detecting sexually transmitted infection (STI) pathogens during animal husbandry, due in part to difficulties associated with the limited infrastructure for veterinary medicine. Here we demonstrate low-cost, multiplexed and sample-to-answer paper-origami tests for the detection of three bovine infectious reproductive diseases in semen samples, collected at a test site in rural India. Pathogen DNA from one viral pathogen, Bovine Herpes virus-1 (BoHV-1) and two bacteria (Brucella and Leptospira) was extracted, amplified (using loop-mediated isothermal amplification, LAMP) and detected fluorescently, enabling