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Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System Xiangmeng Qu, Min Li, Hongbo Zhang, Chenglie Lin, Fei Wang, Mingshu Xiao, Yi Zhou, Jiye Shi, Ali Aldalbahi, Hao Pei, Hong Chen, and Li Li ACS Appl. Mater. Interfaces, Just Accepted Manuscript • DOI: 10.1021/acsami.7b10116 • Publication Date (Web): 31 Aug 2017 Downloaded from http://pubs.acs.org on September 3, 2017
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ACS Applied Materials & Interfaces
Real-Time Continuous Identification of Greenhouse Plant Pathogens Based on Recyclable Microfluidic Bioassay System Xiangmeng Qu,1,2 Min Li,2 Hongbo Zhang,3 Chenglie Lin,4 Fei Wang,5 Mingshu Xiao,2 Yi Zhou,4 Jiye Shi,6 Ali Aldalbahi,7 Hao Pei,2 Hong Chen,1* and Li Li2* KEYWORDS: Real-time, multiple samples detection, microchannel, greenhouse plant pathogens, DNA, hybridization 1
Pen-Tung Sah Institute of Micro-Nano Science and Technology of Xiamen University, Xiamen
University, Xiamen 361005, P. R. China 2
School of Chemistry and Molecular Engineering, East China Normal University, Shanghai
200241, P. R. China 3
Department of Pharmaceutical Sciences Laboratory, Åbo Akademi University, Turku 20520,
Finland 4
School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu
611137, P. R. China
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Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility,
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, P. R. China 6
UCB Pharma, 208 Bath Road, Slough, SL1 3WE, UK
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Chemistry Department, King Saud University, Riyadh 11451, Saudi Arabia
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ACS Applied Materials & Interfaces
ABSTRACT: The development of a real-time continuous analytical platform for the pathogen detection is of great scientific importance for achieving better disease control and prevention. In this work, we report a rapid and recyclable microfluidic bioassay system constructed from oligonucleotide arrays for selective and sensitive continuous identification of DNA targets of fungal pathogens. We employ thermal denaturation method to effectively regenerate the oligonucleotide arrays for multiple sample detection, which could considerably reduce the screening effort and costs. The combination of thermal denaturation and laser-induced fluorescence detection technique enables real-time continuous identification of multiple samples (
