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Residue analysis of sixty pesticides in red swamp crayfish using QuEChERS with high-performance liquid chromatography-tandem mass spectrometry Shuangyu Song, Kechen Zhu, Lijun Han, Yelena Sapozhnikova, Zihao Zhang, and Wei Yao J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b05339 • Publication Date (Web): 24 Jan 2018 Downloaded from http://pubs.acs.org on January 31, 2018
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
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Residue analysis of sixty pesticides in red swamp crayfish using QuEChERS with
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high-performance liquid chromatography-tandem mass spectrometry
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Shuangyu Song1，Kechen Zhu1, Lijun Han*1, Yelena Sapozhnikova2, Zihao Zhang1, Wei Yao1 1
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College of Science, China Agricultural University, Beijing 100193, China
U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center; 600 East Mermaid Lane; Wyndmoor, PA 19038; USA
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Corresponding author： ：
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Lijun Han
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College of Science, China Agricultural University, Beijing 100193, China
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Fax: +86 10 62733620; Tel: +86 10 62733219
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E-mail:
[email protected] 15
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ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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Abstract
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In this study, a multi-residue analytical method using QuEChERS (quick, easy, cheap, effective,
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rugged and safe) extraction and dispersive solid-phase extraction (d-SPE) cleanup followed by
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high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) was
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investigated for rapid determination of 60 pesticide residues in whole crayfish and crayfish meat. The
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final method used 10 mL acetonitrile for extraction, 3 g NaCl for partitioning, and 50 mg PSA for
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d-SPE cleanup. The method was validated at three spiking levels (10, 50 and 100 ng/g) using
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triphenyl phosphate (TPP) as an internal standard and both gradient and isocratic HPLC elution.
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Under gradient conditions, satisfactory recoveries (70-120%) and RSDs≤20% were achieved for 83%
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and 88% of pesticides in whole crayfish and crayfish meat, respectively. Matrix effects were
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estimated using both gradient and isocratic HPLC elution. To our knowledge, this is the first study
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involving multiresidue analysis of HPLC amenable pesticides in crayfish and mantis shrimp. The
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final method was successfully applied for analysis of 11 crayfish and mantis shrimp samples from
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markets in China, and propamocarb (
