Response to Comment on “Biomagnification Study on Organochlorine

Jan 13, 2004 - Response to Comment on “Biomagnification Study on Organochlorine Compounds in Marine Aquaculture: The Sea Bass (Dicentrarchus ...
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Environ. Sci. Technol. 2004, 38, 1263

Response to Comment on “Biomagnification Study on Organochlorine Compounds in Marine Aquaculture: The Sea Bass (Dicentrarchus labrax) as a Model” With regards to the comments of Zitko (1) concerning our paper (2), we respond as follows: (i) The limit of detection of the method is described on page 3377 as 0.1 ng g-1 in fresh fishes for all analytes detected in the paper. This detection limit was deduced from analysis of samples, as blank samples are not available, identifying with MS/MS each peak considered positive detection. Lipidbased concentrations are only a conversion from data obtained in fresh fish analysis. Data presented in the paper are correct. (ii) In further publications, the seasonal changes in fat and xenobiotics contents will be studied. (iii) As we indicated in our paper (2), the scarce visceral fat in commercial cultured fish did not allow the determination of analytes. (iv) We think that the importance of “growth dilution” is very limited as data are expresed in ng g-1.

10.1021/es030670f CCC: $27.50 Published on Web 01/13/2004

 2004 American Chemical Society

(v) For us, PCA analysis is a useful tool for understanding and confirming conclusions from experimental data.

Literature Cited (1) Zitko, V. Environ. Sci. Technol. 2004, 38, 000-000. (2) Serrano, R.; Simal-Julia´n, A.; Pitarch, E.; Herna´ndez, F.; Varo´, I.; Navarro, J. C. Environ. Sci. Technol. 2003, 37, 3375-3381.

Roque Serrano,* Angela Simal-Julia´ n, Pitarch, and Fe´ lix Herna´ ndez

Elena

Analytical Chemistry Department Experimental Sciences University Jaume I P.O. Box 8029 AP 12080 Castello´n, Spain

Inmaculada Varo´ and Juan C. Navarro Institute of Aquaculture of Torre la Sal C.S.I.C. 12595 Ribera de Cabanes Castello´n, Spain ES030670F

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