Environ. Sci. Technol. 2008, 42, 1665–1672
Selective Enzyme-Linked Immunosorbent Assay for Triclosan. Application to Wastewater Treatment Plant Effluents EVA M. BRUN,† EMILIO BONET,‡ R O S A P U C H A D E S , * ,† A N D Á N G E L M A Q U I E I R A * ,† Departamento de Química, Instituto de Química Molecular Aplicada, Universidad Politécnica de Valencia, Camino de Vera s/n, 46071 Valencia, Spain, and Gamaser, S. L., Pedrapiquers, 4, 46014 Valencia, Spain
Received September 14, 2007. Revised manuscript received November 30, 2007. Accepted December 6, 2007.
A sensitive and highly selective enzyme-linked immunosorbent assay was developed for triclosan, one of the most common bactericides in personal care products, which is also considered as an emerging contaminant, given its presence in the effluents of sewage and wastewater treatment plants. From synthesized haptens, polyclonal rabbit antibodies against triclosan were raised. The best ELISA immunoassay was based on an antibody-coated format, yielding a detection limit of 0.03 µg L-1, an I50 of 3.85 µg L-1, and a dynamic range from 0.22 to 42.16 µg L-1, with little or no cross-reactivity (
