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Bioconjugate Chem. 2008, 19, 882–890
Self-Assembled Cationic Nanogels for Intracellular Protein Delivery Hirohito Ayame, Nobuyuki Morimoto, and Kazunari Akiyoshi* Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-0062, Japan. Received November 20, 2007; Revised Manuscript Received February 8, 2008
An effective intracellular protein delivery system with self-assembled cationic nanogels is reported. Interaction of proteins with self-assembled nanogels of cationic cholesteryl group-bearing pullulans (CHPNH2) was investigated by dynamic light scattering (DLS), transmission electron micrograph (TEM), fluorescence resonance energy transfer (FRET), and fluorescence correlation spectroscopy (FCS). The cationic nanogels strongly interacted with bovine serum albumin (BSA) and formed monodispersed nanoparticels (
