Show me a lab that needs advanced HPLC capabilities, and I'll show you a lab that can achieve them with Intelligent LC The UltiMate™ 3000 Intelligent LC Series from Dionex delivers tandem LC, parallel LC, 2-D LC, and on-line SPE-LC.
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These are the world's only HPLCs capable of dual-pump configurations to give you two systems in one, and their software tools provide advanced chromatographic capabilities. Increase throughput by up to 100% running M &
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tandem or parallel LC. Automate sample Bby
preparation using on-line SPE. Separate complex mixtures with 2-D LC. Easy-to-use UitiMate 3000 HPLCs last longer and
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pertorm more reliably. Now, that's productivity.
Passion. Power. Prductivity ©2006 Dionex Corp. PIN 898 UltiMate is a trademark of Dionex Corporation.
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A p p l i c a t i o n s
ProSwift Monolith Columns Deliver Fast Protein Analysis Monolith columns containing an uninterrupted network of interconnected channels are proving to be an important new technology for protein analysis because the monolith structure supports fast analyte mass transfer. Small molecules are quickly separated using conventional porous silica and polymer bead packing in HPLC columns, but proteins and other large biomolecules diffuse slowly, resulting in broad peaks. Convective flow of the monolith is superior to the slow mass transfer by diffusion associated with traditional columns packed with porous beads. ProSwift Monolith columns, from Dionex Corporation, feature a unique monolith morphology that delivers high permeability, low backpressure, and high flow rates,
resulting in high-speed and highresolution HPLC separations of proteins and other large biomolecules. ProSwift columns therefore have higher productivity than columns packed with conventional porous silica and polymer beads. The monoliths provide excellent chemical stability, allowing use of a wide pH range for separation and regeneration. Monolith resin is highly cross-linked for physical stability and long column life. The figure below compares the resolution of five standard proteins on the ProSwift RP2H column with a competitor's column at different flow rates (2 and 8 mL/rmin).
"Show me a lab that needs advanced bioseparations, and I'll show you.a lab that can achieve them with ProSwift™ Monolith columns." The ProSwift Monolith columns use a unique monolith packing for fast, high resolution HPLC and LC/MS
For more information about ProSwift Monolithic columns go to www.dionex.com and click on the ProSwift feature.
separations of proteins, peptides, oligonucleotides, and other biomolecules. This packing has several advantages over traditional packings for a variety of applications. The ProSwift monolith columns offer
Columns:
(1) ProSwift RP-2H (4.6 x 50 mm) (2) Competitor A (4.6x100 mm) Flow Rate: 2 or 8 mL/min Eluents: A. Dl H20 / CH3CN (95:5; V/V) + 0.1 % TFA B. Dl H20 / CH3CN (5:95; V/V) + 0.1 % TFA Detection: UVat214nm
both the high resolving power of
Mixture of 5 proteins Sample: 5uL Inj. Volume: Order of Elution: 1. Ribonuclease A 2. Cytochrome c 3.BSA 4. Carbonic anhydrase 5. Ovalbumin
nonporous media and the high capacity of conventional porous media. Monolithic media not only ensure high resolving power and high capacity, but also high speed
2 mL/min
and low backpressure.
mAU
DIONEX 22904
Passion. Power. Productivity. www.dionex.com
Comparison of ProSwift Monolith with porous HPLC media.
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