1026
Anal. Chem. 1981, 53, 1026-1030
and 5.0 pg mL-' by the "rec-assay" and 10 pg mL-' by the Ames test for AF-2. Oxygen Oxygen As described above, the recombinant-deficient (Rec-) electrode electrode Bacillus bacteria are killed with mutagens. Therefore, a new bacteria Bacillus FEfIT membrane is needed for each analysis; but the preparation F ~ O $ O ~80 Recl . . ) . "TE . . . O.0"*
[email protected] * NRec- NRec+ 1.$'' .&&'0.8@~@1 *. and exchange of a bacteria membrane are very easy and replacement takes only 1min. Moreover, the bacteria can be + Chemical mutagen (AF-2,Mitomycin) i stored for 6 months a t -20 "C. The electrode current was DNA damage DNA damage reproducible within 5% in the buffer solution containing 1 g L-' glucose when the bacteria membrane used for analysis J. 4 was replaced with a new membrane prepared from the stock Death Recombination bacteria. 4 wpair c The microbial electrode system appears promising and ~ ~ ~~ ~~ ~ ~~ ~- ~ 8 0 8 0 $ 0 5 ) 0 ~ Lo o O o O 8 OJ N attractive for use in the routine preliminary screening of mutagens and carcinogens. Further developmental studies NRect NtRec+ N : Number o f c e l l s NRec->NoRecin this laboratory are being directed toward applying the IRect I : Current o f IRec-
