measurements (one for the reference signal and one for the sample signal) are made for each transmittance measurement, then Equation 39 can be used if (1 T-* - T-I) is replaced by (1 T-*). Absorbance Readout. Combination of Equations 10, 12, 26, 28, 29, 31, 32, 40, and A-4 yields, after division by A and rearrangement,
+
+
a,/A K(i,
= {(-In T')'[(ir)-'(@Yir(l + T/(T?)') +
if)/(T'y
+ ir'({,2
( t e i e / T ' ) 2 + (