Size and Chemistry Controlled Cobalt-Ferrite ... - ACS Publications

Sep 20, 2016 - Department of Zoology, Savitribai Phule Pune University, Ganeshkhind Road, Pune-411007, Maharashtra India. §. Solid State Physics Divi...
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Size and Chemistry Controlled Cobalt-Ferrite Nanoparticles and Their Anti-Proliferative Effect against the MCF-7 Breast Cancer Cells Sumayya Moinuddin Ansari, Renuka D. Bhor, Kalpana R. Pai, Subhasish Mazumder, Debasis Sen, Yesh D. Kolekar, and Chintalapalle V Ramana ACS Biomater. Sci. Eng., Just Accepted Manuscript • DOI: 10.1021/acsbiomaterials.6b00333 • Publication Date (Web): 20 Sep 2016 Downloaded from http://pubs.acs.org on September 22, 2016

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ACS Biomaterials Science & Engineering

Size and Chemistry Controlled Cobalt-Ferrite Nanoparticles and Their AntiProliferative Effect against the MCF-7 Breast Cancer Cells Sumayya M. Ansari,1 Renuka D. Bhor,2 Kalpana R. Pai,2 Subhasish Mazumder,3 Debasis Sen,3 Yesh D. Kolekar1,# and C. V. Ramana* 1

Department of Physics, Savitribai Phule Pune University, Ganeshkhind Road, Pune-411007, Maharashtra, India

2

Department of Zoology, Savitribai Phule Pune University, Ganeshkhind Road, Pune-411007, Maharashtra, India 3

Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400 085, India

4

Department of Mechanical Engineering, University of Texas at El Paso, 500 W. Univ. Ave., El Paso, Texas 79968, United States

*,#

Corresponding Author: [email protected] (C.V. Ramana) [email protected] (Y.D. Kolekar) C.V. Ramana: Tel: +1-9157478690-2678; Fax: +1-9157475019 Y.D. Kolekar: Tel: +91-20-2569-2678; Fax: +91-20-2569-1684

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ACS Biomaterials Science & Engineering

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ABSTRACT Engineering cobalt ferrites for application in health and biomedical science poses a challenge in terms of nanoscale morphology with a controlled size, shape and thermochemical stability coupled with controlled properties for bio-compatibility. Here we report a simple one-step, low temperature approach to produce crystalline, nano-sized cobalt ferrites (CFO) with a size ~ 4.7 nm and demonstrate their applicability in breast cancer treatment. Inherent physiochemical and magnetic properties, which are quite important for biomedical applications, along with cytotoxicity of CFO nanoparticles (NPs) are investigated in detail. X-ray diffraction analyses confirm the cubic spinel phase with the tensile strain in crystalline CFO NPs. Chemical bonding analyses using Infrared and Raman spectroscopic studies also support the cubic spinel phase. Electron microscopy and small-angle X-ray scattering revealed the narrow particle-size distribution and spherical-shape morphology. The as-synthesized CFO NPs exhibit superparamagnetic character. Unsaturated magnetization behavior suggests the existence of disordered spins in the surface layers. The temperature dependence of the magnetic parameters namely, saturation magnetization, coercivity, retentivity and squareness ratio, also supports the surface-localized spins. Cytotoxic activity of the as-synthesized CFO NPs against the human breast cancer (MCF-7) cell line and normal human peripheral blood mononuclear cells (PBMC) has been evaluated. The mild response of CFO NPs in terms of their anti-proliferative nature against cancer cells and negligible Cytotoxicity reflecting their human-safe-and-friendly nature makes them suitable for bio-applications. Moreover, assessment of toxicity toward human red blood cells (RBC) revealed (