Subscriber access provided by CORNELL UNIVERSITY LIBRARY
Article
Size and Chemistry Controlled Cobalt-Ferrite Nanoparticles and Their Anti-Proliferative Effect against the MCF-7 Breast Cancer Cells Sumayya Moinuddin Ansari, Renuka D. Bhor, Kalpana R. Pai, Subhasish Mazumder, Debasis Sen, Yesh D. Kolekar, and Chintalapalle V Ramana ACS Biomater. Sci. Eng., Just Accepted Manuscript • DOI: 10.1021/acsbiomaterials.6b00333 • Publication Date (Web): 20 Sep 2016 Downloaded from http://pubs.acs.org on September 22, 2016
Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.
ACS Biomaterials Science & Engineering is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
Page 1 of 58
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60
ACS Biomaterials Science & Engineering
Size and Chemistry Controlled Cobalt-Ferrite Nanoparticles and Their AntiProliferative Effect against the MCF-7 Breast Cancer Cells Sumayya M. Ansari,1 Renuka D. Bhor,2 Kalpana R. Pai,2 Subhasish Mazumder,3 Debasis Sen,3 Yesh D. Kolekar1,# and C. V. Ramana* 1
Department of Physics, Savitribai Phule Pune University, Ganeshkhind Road, Pune-411007, Maharashtra, India
2
Department of Zoology, Savitribai Phule Pune University, Ganeshkhind Road, Pune-411007, Maharashtra, India 3
Solid State Physics Division, Bhabha Atomic Research Centre, Trombay, Mumbai-400 085, India
4
Department of Mechanical Engineering, University of Texas at El Paso, 500 W. Univ. Ave., El Paso, Texas 79968, United States
*,#
Corresponding Author:
[email protected] (C.V. Ramana)
[email protected] (Y.D. Kolekar) C.V. Ramana: Tel: +1-9157478690-2678; Fax: +1-9157475019 Y.D. Kolekar: Tel: +91-20-2569-2678; Fax: +91-20-2569-1684
ACS Paragon Plus Environment
ACS Biomaterials Science & Engineering
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60
ABSTRACT Engineering cobalt ferrites for application in health and biomedical science poses a challenge in terms of nanoscale morphology with a controlled size, shape and thermochemical stability coupled with controlled properties for bio-compatibility. Here we report a simple one-step, low temperature approach to produce crystalline, nano-sized cobalt ferrites (CFO) with a size ~ 4.7 nm and demonstrate their applicability in breast cancer treatment. Inherent physiochemical and magnetic properties, which are quite important for biomedical applications, along with cytotoxicity of CFO nanoparticles (NPs) are investigated in detail. X-ray diffraction analyses confirm the cubic spinel phase with the tensile strain in crystalline CFO NPs. Chemical bonding analyses using Infrared and Raman spectroscopic studies also support the cubic spinel phase. Electron microscopy and small-angle X-ray scattering revealed the narrow particle-size distribution and spherical-shape morphology. The as-synthesized CFO NPs exhibit superparamagnetic character. Unsaturated magnetization behavior suggests the existence of disordered spins in the surface layers. The temperature dependence of the magnetic parameters namely, saturation magnetization, coercivity, retentivity and squareness ratio, also supports the surface-localized spins. Cytotoxic activity of the as-synthesized CFO NPs against the human breast cancer (MCF-7) cell line and normal human peripheral blood mononuclear cells (PBMC) has been evaluated. The mild response of CFO NPs in terms of their anti-proliferative nature against cancer cells and negligible Cytotoxicity reflecting their human-safe-and-friendly nature makes them suitable for bio-applications. Moreover, assessment of toxicity toward human red blood cells (RBC) revealed (
