Biochemistry , 1971, 10 (24), pp 4503â4508. DOI: 10.1021/bi00800a025. Publication Date: November 1971 ... Photochemistry and Photobiology 1980 32 (5), 593-600 .... Can stripping the air of its moisture quench the world's thirst? We live in ...
Mauricio Lasagna, Victor Vargas, David M. Jameson, and Juan E. Brunet*. Instituto de QuÃmica, Universidad Católica de Valparaiso, Casilla 4059, Valparaiso, ...
Nov 17, 1995 - Mauricio Lasagna,‡ Victor Vargas,§ David M. Jameson,| and Juan E. Brunet*,‡. Instituto de Quı´mica, UniVersidad Cato´lica de Valparaiso, ...
The excited state dipole of PRODAN, originally estimated at 18â20 D (Weber & Farris, 1979), has more recently been estimated as 7â8 D (Balter et al., 1988; ...
Jan 23, 1996 - We attribute these lifetime distributions to microenvironmental heterogeneity which is also consistent with the observed dependence of the emission maxima of PRODANâapohorseradish peroxidase upon the excitation wavelength. In neither
Nov 17, 1995 - Mauricio Lasagna,â¡ Victor Vargas,§ David M. Jameson,| and Juan E. Brunet*,â¡. Instituto de Quı´mica, UniVersidad Cato´lica de Valparaiso, ...
(25) (a) Henry, B. R.; Siebrand, W. Organic Molecular Photophysics; Birks: London, 1973; Vol. 1. (b) Fong, F. K. Topics in Applied Physics;. Springer: New York ...
Sane, R., Goodchild, D., and Park, R. B. (1970), Biochim. Biophys. Acta 216,162. Senger, H. (1970), Planta 92, 327. Tanner, W., Loffler, M., and Kandler, ...
13 Jul 2005 - The absorption, fluorescence excitation, and fluorescence emission spectra of water solutions of fluorescein dye with the addition of various ...
Jan 21, 2015 - We have examined anode materials with anions as an ion transport species to solve metal deposition in rechargeable batteries intrinsically.
PURIFICATION OF A DNA PHOTOLYASE
from phosphocellulose columns as described by Eley (1969). By this technique we have obtained milligram amounts of essentially pure enzyme and there appear no technical limitations to scaling up the process for the production of larger amounts. The uses and future of phosphocellulose columns in the purification of enzymes which act on nucleic acids have been discussed by Eley ; however one difficulty arises in the phosphocellulose chromatography of the Lactobacillus enzyme. After elution of the enzyme with R N A from the first phosphocellulose column and throughout the second chromatography the presence of contaminating R N A interferes dramatically with the enzyme assay. In contrast to Eley's studies with chicken pancreas nuclease the R N A is not removed completely on the second (salt elution) column. We have resorted to extended incubation periods allowing degradation of the accompanying R N A followed by dialysis to eliminate the RNA. Unfortunately contaminating R N A (
